دورية أكاديمية
أعرض في EDS

Guttiferone BL from the Fruits of Allanblackia gabonensis Induces Mitochondrial-Dependent Apoptosis in PA-1 Ovarian Cancer Cells.

التفاصيل البيبلوغرافية
العنوان: Guttiferone BL from the Fruits of Allanblackia gabonensis Induces Mitochondrial-Dependent Apoptosis in PA-1 Ovarian Cancer Cells.
المؤلفون: Fankam AG; Department of Biochemistry, University of Dschang, P.O. Box 67, Dschang, Cameroon.; Cancer Biology and Inflammatory Disorder Division, Council of Scientific and Industrial- (CSIR-) Indian Institute of Chemical Biology, 4, Raja S.C. Mullick Road, Jadavpur, 700032, Kolkata, India., Mondal S; Department of Zoology, Diamond Harbour Women's University, Sarisha, West Bengal 743368, India., Dongmo FLM; University Institute of Technology, University of Ngaoundéré, P.O. Box 455, Ngaoundéré, Cameroon., Nganou BK; Department of Chemistry, University of Dschang, P.O. Box 67, Dschang, Cameroon., Konga IS; Department of Chemistry, University of Dschang, P.O. Box 67, Dschang, Cameroon., Mandal C; Cancer Biology and Inflammatory Disorder Division, Council of Scientific and Industrial- (CSIR-) Indian Institute of Chemical Biology, 4, Raja S.C. Mullick Road, Jadavpur, 700032, Kolkata, India.; Department of Zoology, Diamond Harbour Women's University, Sarisha, West Bengal 743368, India., Kuete V; Department of Biochemistry, University of Dschang, P.O. Box 67, Dschang, Cameroon.
المصدر: BioMed research international [Biomed Res Int] 2023 Feb 21; Vol. 2023, pp. 8981430. Date of Electronic Publication: 2023 Feb 21 (Print Publication: 2023).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Hindawi Pub. Co Country of Publication: United States NLM ID: 101600173 Publication Model: eCollection Cited Medium: Internet ISSN: 2314-6141 (Electronic) NLM ISO Abbreviation: Biomed Res Int Subsets: MEDLINE
أسماء مطبوعة: Original Publication: New York, NY : Hindawi Pub. Co.
مواضيع طبية MeSH: Apoptosis* , Fruit*/chemistry , Ovarian Neoplasms*/drug therapy , Benzophenones*/pharmacology, Female ; Humans ; Cell Line, Tumor
مستخلص: Despite the recent advancement of treatment strategies, cancer ranks 2 nd among the causes of death globally. Phytochemicals have gained popularity as an alternate therapeutic strategy due to their nontoxic nature. Here, we have investigated the anticancer properties of guttiferone BL (GBL) along with four known compounds previously isolated from Allanblackia gabonensis . The cytotoxicity was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. The study was extended for the assessment of the effect of GBL in PA-1 cells apoptosis induction, cell cycle distribution, and change in mitochondrial membrane potential using flow cytometry, Western blot analysis, and real-time PCR. Among the five tested compounds, GBL displayed significant antiproliferative effects against all tested human cancer cells (IC 50 < 10 μ M). Moreover, GBL exhibited no significant cytotoxicity towards normal ovarian epithelial cell line (IOSE 364) up to 50  μ M. GBL induced sub-G 0 cell cycle arrest and significant upregulation of cell cycle regulatory proteins of ovarian cancer cell PA-1. Furthermore, GBL induced its apoptosis as depicted by the accumulation of cells both at the early and late apoptotic phase in Annexin V/PI assay. In addition, it decreased the PA-1 mitochondrial membrane potential and promoted upregulation of caspase-3, caspase-9, and Bax and downregulation of Bcl-2. GBL also showed a dose-dependent inhibition of PA-1 migration. Altogether, this study reveals that guttiferone BL, studied herein for the first time, exhibits efficient antiproliferative activity by the induction of apoptosis through the mitochondrial-dependent pathway. Its investigation as a therapeutic agent against human cancers especially ovarian cancer should be envisaged.
Competing Interests: The authors declare that they have no known competing interests.
(Copyright © 2023 Aimé Gabriel Fankam et al.)
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المشرفين على المادة: 0 (guttiferone G)
0 (Benzophenones)
تواريخ الأحداث: Date Created: 20230303 Date Completed: 20230307 Latest Revision: 20230307
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC9974259
DOI: 10.1155/2023/8981430
PMID: 36865482
قاعدة البيانات: MEDLINE
الوصف
تدمد:2314-6141
DOI:10.1155/2023/8981430