دورية أكاديمية
أعرض في EDS

Occurrence and prevalence of antimicrobial resistance in urban karst groundwater systems based on targeted resistome analysis.

التفاصيل البيبلوغرافية
العنوان: Occurrence and prevalence of antimicrobial resistance in urban karst groundwater systems based on targeted resistome analysis.
المؤلفون: Kaiser RA; School of Environmental Studies, College of Interdisciplinary Studies, Tennessee Technological University, 1 William L Jones Drive, Cookeville, TN 38505, United States. Electronic address: rakaiser42@tntech.edu., Polk JS; Earth, Environmental, and Atmospheric Sciences Department, Ogden College of Science and Engineering, 1906 College Heights Blvd., Bowling Green, KY 42101, United States., Datta T; Department of Civil and Environmental Engineering, College of Engineering, Tennessee Technological University, 1 William L Jones Drive, Cookeville, TN 38505, United States., Keely SP; United States Environmental Protection Agency, 26 Martin Luther King Drive West, Cincinnati, OH 45220, United States., Brinkman NE; United States Environmental Protection Agency, 26 Martin Luther King Drive West, Cincinnati, OH 45220, United States., Parekh RR; Food Animal Environmental Systems Research Unit, Agricultural Research Service, United States Department of Agriculture, 2413 Nashville Road B5, Bowling Green, KY 42101, United States., Agga GE; Food Animal Environmental Systems Research Unit, Agricultural Research Service, United States Department of Agriculture, 2413 Nashville Road B5, Bowling Green, KY 42101, United States.
المصدر: The Science of the total environment [Sci Total Environ] 2023 May 20; Vol. 874, pp. 162571. Date of Electronic Publication: 2023 Mar 05.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: Netherlands NLM ID: 0330500 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-1026 (Electronic) Linking ISSN: 00489697 NLM ISO Abbreviation: Sci Total Environ Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Amsterdam, Elsevier.
مواضيع طبية MeSH: Groundwater* , Drinking Water*, Animals ; Humans ; Anti-Bacterial Agents/pharmacology ; Prevalence ; Drug Resistance, Bacterial ; Genes, Bacterial
مستخلص: Antimicrobial resistance (AMR) is a global crisis threatening human, animal, and environmental health. The natural environment, specifically water resources, has been recognized as a reservoir and dissemination pathway for AMR; however, urban karst aquifer systems have been overlooked. This is a concern as these aquifer systems provide drinking water to about 10 % of the global population; yet, the urban influence on the resistome in these vulnerable aquifers is sparingly explored. This study used high-throughput qPCR to determine the occurrence and relative abundance of antimicrobial resistant genes (ARG) in a developing urban karst groundwater system in Bowling Green, KY. Ten sites throughout the city were sampled weekly and analyzed for 85 ARGs, as well as seven microbial source tracking (MST) genes for human and animal sources, providing a spatiotemporal understanding of the resistome in urban karst groundwater. To further understand ARGs in this environment, potential drivers (landuse, karst feature type, season, source of fecal pollution) were considered in relation to the resistome relative abundance. The MST markers highlighted a prominent human influence to the resistome in this karst setting. The concentration of targeted genes varied between the sample weeks, but all targeted ARGs were prevalent throughout the aquifer regardless of karst feature type or season, with high concentrations captured for sulfonamide (sul1), quaternary ammonium compound (qacE), and aminoglycoside (strB) antimicrobial classes. Higher prevalence and relative abundance were detected during the summer and fall seasons, as well as at the spring features. Linear discriminant analysis suggested that karst feature type had higher influence on ARGs in the aquifer compared to season and the source of fecal pollution had the least influence. These findings can contribute to the development of effective management and mitigation strategies for AMR.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier B.V. All rights reserved.)
References: Sci Total Environ. 2020 May 1;715:136975. (PMID: 32018106)
Sci Total Environ. 2019 Dec 20;697:134023. (PMID: 31479900)
Antibiotics (Basel). 2022 Jan 05;11(1):. (PMID: 35052940)
Environ Int. 2018 Aug;117:146-153. (PMID: 29751164)
Environ Sci Pollut Res Int. 2019 Sep;26(27):28352-28360. (PMID: 31372954)
Environ Int. 2018 Dec;121(Pt 2):1155-1161. (PMID: 30420129)
Methods. 2001 Dec;25(4):402-8. (PMID: 11846609)
Sci Total Environ. 2020 Mar 1;706:135730. (PMID: 31791761)
Infect Dis Clin North Am. 2016 Jun;30(2):313-322. (PMID: 27208761)
Water Res. 2020 Dec 15;187:116455. (PMID: 33032106)
Int J Environ Res Public Health. 2022 May 27;19(11):. (PMID: 35682132)
Sci Total Environ. 2014 Aug 15;490:1082-90. (PMID: 24922611)
Environ Pollut. 2021 Oct 15;287:117651. (PMID: 34426396)
Chemosphere. 2022 Aug;301:134707. (PMID: 35487363)
Environ Pollut. 2016 Jun;213:119-126. (PMID: 26890482)
Microbiome. 2017 Nov 28;5(1):154. (PMID: 29179769)
Turk J Emerg Med. 2018 Aug 07;18(3):91-93. (PMID: 30191186)
Sci Total Environ. 2019 Nov 25;693:133585. (PMID: 31377359)
Ground Water. 2019 Sep;57(5):807-818. (PMID: 31297792)
FEMS Microbiol Ecol. 2018 Jul 1;94(7):. (PMID: 29767712)
Appl Microbiol Biotechnol. 2009 Mar;82(3):397-414. (PMID: 19130050)
J Environ Qual. 2016 Mar;45(2):394-406. (PMID: 27065386)
Water Res. 2022 Oct 1;224:119060. (PMID: 36096030)
Infect Drug Resist. 2018 Oct 10;11:1645-1658. (PMID: 30349322)
Sci Total Environ. 2020 Nov 10;742:140529. (PMID: 32629259)
Sci Total Environ. 2014 Aug 15;490:370-8. (PMID: 24875257)
Can J Microbiol. 2020 Jan;66(1):46-58. (PMID: 31622563)
Environ Pollut. 2017 Nov;230:648-654. (PMID: 28715769)
Appl Environ Microbiol. 2015 Aug;81(15):5037-47. (PMID: 26002893)
Sci Total Environ. 2019 Jun 15;669:785-797. (PMID: 30897437)
Environ Sci Pollut Res Int. 2017 Oct;24(30):23753-23762. (PMID: 28864929)
Water Res. 2020 Mar 1;170:115360. (PMID: 31830652)
FEMS Microbiol Ecol. 2016 Mar;92(3):. (PMID: 26832203)
J Microbiol Methods. 2021 May;184:106174. (PMID: 33774111)
Trends Microbiol. 2018 Mar;26(3):220-228. (PMID: 29033338)
Environ Pollut. 2018 May;236:734-744. (PMID: 29454283)
Water Res. 2021 Sep 15;203:117507. (PMID: 34392041)
Microbiol Spectr. 2016 Apr;4(2):. (PMID: 27227291)
Environ Int. 2022 Apr;162:107171. (PMID: 35290866)
Trop Med Infect Dis. 2020 Feb 25;5(1):. (PMID: 32106595)
Molecules. 2019 Jan 03;24(1):. (PMID: 30609875)
J Environ Qual. 2002 Jul-Aug;31(4):1300-8. (PMID: 12175050)
Toxicol Rep. 2021 Mar 09;8:646-656. (PMID: 33868951)
معلومات مُعتمدة: EPA999999 United States ImEPA Intramural EPA
فهرسة مساهمة: Keywords: Antimicrobial resistance genes; High-throughput qPCR; Urban Resistome; Urban karst groundwater
المشرفين على المادة: 0 (Anti-Bacterial Agents)
0 (Drinking Water)
تواريخ الأحداث: Date Created: 20230305 Date Completed: 20230329 Latest Revision: 20240521
رمز التحديث: 20240521
مُعرف محوري في PubMed: PMC10449245
DOI: 10.1016/j.scitotenv.2023.162571
PMID: 36871706
قاعدة البيانات: MEDLINE
الوصف
تدمد:1879-1026
DOI:10.1016/j.scitotenv.2023.162571