دورية أكاديمية
أعرض في EDS

Preferential production and secretion of the complement regulator factor H-like protein 1 (FHL-1) by human myeloid cells.

التفاصيل البيبلوغرافية
العنوان: Preferential production and secretion of the complement regulator factor H-like protein 1 (FHL-1) by human myeloid cells.
المؤلفون: van Essen MF; Division of Nephrology and Transplant Medicine, Department of Medicine, Leiden University Medical Center, Leiden, the Netherlands. Electronic address: m.f.van_essen@lumc.nl., Peereboom ETM; Division of Nephrology and Transplant Medicine, Department of Medicine, Leiden University Medical Center, Leiden, the Netherlands., Schlagwein N; Division of Nephrology and Transplant Medicine, Department of Medicine, Leiden University Medical Center, Leiden, the Netherlands., van Gijlswijk-Janssen DJ; Division of Nephrology and Transplant Medicine, Department of Medicine, Leiden University Medical Center, Leiden, the Netherlands., Nelemans T; Division of Nephrology and Transplant Medicine, Department of Medicine, Leiden University Medical Center, Leiden, the Netherlands., Joeloemsingh JV; Division of Nephrology and Transplant Medicine, Department of Medicine, Leiden University Medical Center, Leiden, the Netherlands., van den Berg CW; Division of Nephrology and Transplant Medicine, Department of Medicine, Leiden University Medical Center, Leiden, the Netherlands., Prins J; Division of Nephrology and Transplant Medicine, Department of Medicine, Leiden University Medical Center, Leiden, the Netherlands., Clark SJ; Institute for Ophthalmic Research, Eberhard Karls University of Tübingen, Tübingen, Baden-Württemberg 72076, Germany; Lydia Becker Institute of Immunology and Inflammation, Faculty of Biology, Medicine & Health, University of Manchester, Manchester M13 9PL, United Kingdom., Schmidt CQ; Institute of Pharmacology of Natural Products and Clinical Pharmacology, Ulm University, Ulm, Germany., Trouw LA; Department of Immunology, Leiden University Medical Center, Leiden, the Netherlands., van Kooten C; Division of Nephrology and Transplant Medicine, Department of Medicine, Leiden University Medical Center, Leiden, the Netherlands. Electronic address: c.van_kooten@lumc.nl.
المصدر: Immunobiology [Immunobiology] 2023 May; Vol. 228 (3), pp. 152364. Date of Electronic Publication: 2023 Feb 28.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: Netherlands NLM ID: 8002742 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1878-3279 (Electronic) Linking ISSN: 01712985 NLM ISO Abbreviation: Immunobiology Subsets: MEDLINE
أسماء مطبوعة: Publication: <2005->: Amsterdam : Elsevier
Original Publication: Stuttgart ; New York, Fischer.
مواضيع طبية MeSH: Complement Factor H*/genetics , Complement Activation*, Humans ; Myeloid Cells/metabolism ; RNA, Messenger ; Muscle Proteins ; Intracellular Signaling Peptides and Proteins ; LIM Domain Proteins
مستخلص: Factor H is a pivotal complement regulatory protein that is preferentially produced by the liver and circulates in high concentrations in serum. There has been an increasing interest in the extrahepatic production of complement factors, including by cells of the immune system, since this contributes to non-canonical functions of local complement activation and regulation. Here we investigated the production and regulation of factor H and its splice variant factor H-like protein 1 (FHL-1) by human myeloid cells. As validation, we confirmed the predominant presence of intact factor H in serum, despite a strong but comparable mRNA expression of CFH and FHL1 in liver. Comparable levels of CFH and FHL1 were also observed in renal tissue, although a dominant staining for FHL-1 was shown within the proximal tubules. Human in vitro generated pro- and anti-inflammatory macrophages both expressed and produced factor H/FHL-1, but this was strongest in pro-inflammatory macrophages. Production was not affected by LPS activation, but was increased upon stimulation with IFN-γ or CD40L. Importantly, in both macrophage subsets mRNA expression of FHL1 was significantly higher than CFH. Moreover, production of FHL-1 protein could be confirmed using precipitation and immunoblotting of culture supernatants. These data identify macrophages as producers of factor H and FHL-1, thereby potentially contributing to local complement regulation at sites of inflammation.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 The Authors. Published by Elsevier GmbH.. All rights reserved.)
فهرسة مساهمة: Keywords: Complement; Factor H/FHL-1; Human; Inflammation; Monocyte/Macrophages
المشرفين على المادة: 80295-65-4 (Complement Factor H)
0 (RNA, Messenger)
0 (FHL1 protein, human)
0 (Muscle Proteins)
0 (Intracellular Signaling Peptides and Proteins)
0 (LIM Domain Proteins)
تواريخ الأحداث: Date Created: 20230307 Date Completed: 20230614 Latest Revision: 20230615
رمز التحديث: 20230615
DOI: 10.1016/j.imbio.2023.152364
PMID: 36881973
قاعدة البيانات: MEDLINE
الوصف
تدمد:1878-3279
DOI:10.1016/j.imbio.2023.152364