دورية أكاديمية
UPLC-QTOF-MS/MS-based metabolomic approach and gastroprotective effect of two chemotypes of Egletes viscosa (L.) less. against ethanol-induced gastric ulcer in mice.
| العنوان: | UPLC-QTOF-MS/MS-based metabolomic approach and gastroprotective effect of two chemotypes of Egletes viscosa (L.) less. against ethanol-induced gastric ulcer in mice. |
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| المؤلفون: | Santos FA; Department of Physiology and Pharmacology, Faculty of Medicine, Federal University of Ceará, Fortaleza, Ceará, Brazil. Electronic address: flavia@ufc.br., Viana AFSC; Department of Physiology and Pharmacology, Faculty of Medicine, Federal University of Ceará, Fortaleza, Ceará, Brazil. Electronic address: flavia_seraine@hotmail.com., Nunes PIG; Department of Physiology and Pharmacology, Faculty of Medicine, Federal University of Ceará, Fortaleza, Ceará, Brazil. Electronic address: pign23@gmail.com., Portela BYM; Department of Physiology and Pharmacology, Faculty of Medicine, Federal University of Ceará, Fortaleza, Ceará, Brazil. Electronic address: yagoportelaadm@gmail.com., Alves APNN; Dental Clinic Department, Faculty of Pharmacy, Dentistry and Nursing, Federal University of Ceará, Fortaleza, Ceará, Brazil. Electronic address: ananegreirosnunes@gmail.com., Viana DA; Christhus University Center, Fortaleza, Ceará, Brazil. Electronic address: viana_daniel@yahoo.com.br., Carvalho KR; Brazilian Agricultural Research Corporation (Embrapa), Tropical Agroindustry, Fortaleza, Ceará, Brazil; Department of Organic and Inorganic Chemistry, Federal University of Ceará, Fortaleza, Ceará, Brazil. Electronic address: kaline141@hotmail.com., Pereira RCA; Brazilian Agricultural Research Corporation (Embrapa), Tropical Agroindustry, Fortaleza, Ceará, Brazil. Electronic address: rita.pereira@embrapa.br., Ribeiro PRV; Brazilian Agricultural Research Corporation (Embrapa), Tropical Agroindustry, Fortaleza, Ceará, Brazil. Electronic address: paulo.riceli@embrapa.br., Alves-Filho EG; Brazilian Agricultural Research Corporation (Embrapa), Tropical Agroindustry, Fortaleza, Ceará, Brazil. Electronic address: elenilson.godoy@yahoo.com.br., de Brito ES; Brazilian Agricultural Research Corporation (Embrapa), Tropical Agroindustry, Fortaleza, Ceará, Brazil. Electronic address: edy.brito@embrapa.br., Silveira ER; Department of Organic and Inorganic Chemistry, Federal University of Ceará, Fortaleza, Ceará, Brazil. Electronic address: edil@ufc.br., Canuto KM; Brazilian Agricultural Research Corporation (Embrapa), Tropical Agroindustry, Fortaleza, Ceará, Brazil. Electronic address: kirley.canuto@embrapa.br. |
| المصدر: | Journal of ethnopharmacology [J Ethnopharmacol] 2023 Jun 12; Vol. 309, pp. 116348. Date of Electronic Publication: 2023 Mar 07. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Sequoia Country of Publication: Ireland NLM ID: 7903310 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1872-7573 (Electronic) Linking ISSN: 03788741 NLM ISO Abbreviation: J Ethnopharmacol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Limerick : Elsevier Sequoia Original Publication: Lausanne, Elsevier Sequoia. |
| مواضيع طبية MeSH: | Stomach Ulcer*/chemically induced , Stomach Ulcer*/drug therapy , Stomach Ulcer*/prevention & control , Diterpenes*/pharmacology , Anti-Ulcer Agents*/pharmacology , Anti-Ulcer Agents*/therapeutic use, Mice ; Animals ; Ethanol/pharmacology ; Tandem Mass Spectrometry ; Nitric Oxide/metabolism ; Plant Extracts/pharmacology ; Plant Extracts/therapeutic use ; Plant Extracts/chemistry ; Antioxidants/pharmacology ; Prostaglandins/metabolism ; Flavonoids/pharmacology ; Adenosine Triphosphate/metabolism ; Gastric Mucosa |
| مستخلص: | Ethnopharmacological Relevance: Egletes viscosa (L.) (macela) is a native wild herb that can be found in different states of northeastern Brazil. The infusions of its flower buds are traditionally used for the treatment of gastrointestinal disorders. E. viscosa possesses two chemotypes (named A and B), distinguishable by the composition of the essential oil from the flower buds. Although there are previous studies of the gastroprotective effect of the isolated constituents of E. viscosa, its infusions have not been investigated yet. Aim of the Study: The present study aimed to evaluate and compare the chemical composition and the gastroprotective effect of flower bud infusions of E. viscosa from chemotype A (EVCA) and chemotype B (EVCB). Materials and Methods: Sixteen infusions were brewed with flower buds according to the traditional preparation mode and were analyzed through a UPLC-QTOF-MS/MS based metabolomic approach for determination of their metabolic fingerprints and quantification of bioactive compounds. Afterward, these data were analyzed by chemometric methods (OPLS-DA) for discrimination of the two chemotypes. Additionally, infusions of EVCA and EVCB (50, 100 and 200 mg/kg, p.o.) were evaluated on gastric ulcers induced by absolute ethanol (96%, 0.2 mL, p.o.) in mice. To elucidate the gastroprotective mechanisms, the effect of EVCA and EVCB on gastric acid secretion and gastric wall mucus was determined and the role of TRPV1 channels, prostaglandins, nitric oxide and K Results: The chemotypes can be discriminated from each other using UPLC-QTOF-MS/MS chemical fingerprints. Both chemotypes presented similar chemical compositions, consisting basically of caffeic acid derivatives, flavonoids and diterpenes. The quantification of bioactive compounds demonstrated that chemotype A possesses more ternatin, tanabalin and centipedic than chemotype B. EVCA and EVCB (50, 100 and 200 mg/kg, p.o.) significantly decreased the severity of ethanol-induced gastric lesions, as shown by a reduction in histological alterations and leucocyte infiltration in gastric tissue. The gastroprotective mechanism of both infusions involves an antioxidant effect, maintenance of gastric mucus and reduction gastric secretion. Stimulation of endogenous prostaglandins and nitric oxide release, activation of TRPV1 channels, and K Conclusion: The gastroprotective effect of EVCA and EVCB was equivalent and mediated through antioxidant and antisecretory actions, including the activation of TRPV1 receptors, stimulation of endogenous prostaglandins and nitric oxide, and opening of K Competing Interests: Declaration of competing interest The authors declare that there are no conflicts of interest. (Copyright © 2023 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Diterpenes; Egletes viscosa, gastric ulcer; Flavonoids; Phenolic acids |
| المشرفين على المادة: | U2S3A33KVM (caffeic acid) 3K9958V90M (Ethanol) 31C4KY9ESH (Nitric Oxide) 0 (Plant Extracts) 0 (Antioxidants) 0 (Prostaglandins) 0 (Diterpenes) 0 (Flavonoids) 8L70Q75FXE (Adenosine Triphosphate) 0 (Anti-Ulcer Agents) |
| تواريخ الأحداث: | Date Created: 20230309 Date Completed: 20230327 Latest Revision: 20230327 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/j.jep.2023.116348 |
| PMID: | 36894109 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1872-7573 |
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| DOI: | 10.1016/j.jep.2023.116348 |