دورية أكاديمية
Assessment of Oxidative Stress Biomarkers in Rat Blood.
| العنوان: | Assessment of Oxidative Stress Biomarkers in Rat Blood. |
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| المؤلفون: | Sinzato YK; Laboratory of Experimental Research on Gynecology and Obstetrics, Botucatu Medical School, São Paulo State University (Unesp), Botucatu, São Paulo State, Brazil., Rodrigues T; Center of Natural and Human Sciences (CCNH), Federal University of ABC (UFABC), Santo André, São Paulo State, Brazil., Cruz LL; Laboratory of Experimental Research on Gynecology and Obstetrics, Botucatu Medical School, São Paulo State University (Unesp), Botucatu, São Paulo State, Brazil.; Laboratory of System Physiology and Reproductive Toxicology, Institute of Biological and Health Sciences, Federal University of Mato Grosso (UFMT), Barra do Garças, Mato Grosso State, Brazil., Barco VS; Laboratory of Experimental Research on Gynecology and Obstetrics, Botucatu Medical School, São Paulo State University (Unesp), Botucatu, São Paulo State, Brazil., Souza MR; Laboratory of Experimental Research on Gynecology and Obstetrics, Botucatu Medical School, São Paulo State University (Unesp), Botucatu, São Paulo State, Brazil.; Laboratory of System Physiology and Reproductive Toxicology, Institute of Biological and Health Sciences, Federal University of Mato Grosso (UFMT), Barra do Garças, Mato Grosso State, Brazil., Volpato GT; Laboratory of System Physiology and Reproductive Toxicology, Institute of Biological and Health Sciences, Federal University of Mato Grosso (UFMT), Barra do Garças, Mato Grosso State, Brazil., Damasceno DC; Laboratory of Experimental Research on Gynecology and Obstetrics, Botucatu Medical School, São Paulo State University (Unesp), Botucatu, São Paulo State, Brazil. |
| المصدر: | Bio-protocol [Bio Protoc] 2023 Mar 05; Vol. 13 (5). Date of Electronic Publication: 2023 Mar 05 (Print Publication: 2023). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Bio-protocol LLC Country of Publication: United States NLM ID: 101635102 Publication Model: eCollection Cited Medium: Internet ISSN: 2331-8325 (Electronic) Linking ISSN: 23318325 NLM ISO Abbreviation: Bio Protoc Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Original Publication: Sunnyvale, CA, USA : Bio-protocol LLC, [2011]- |
| مستخلص: | Redox status assessments are time-consuming, require a large volume of samples and great reagent amounts, and are not adequately described for methodological reproducibility. Here, the objective was to standardize redox balance determination, based on previously described spectrophotometric tests in pregnant rats, to improve precision, time dispensed, and the volume of samples and reagents, while maintaining accuracy and adequate cost benefits. This protocol summarizes oxidative stress markers, which focus on spectrophotometric tests for the assessment of thiobarbituric acid-reactive substances, reduced thiol groups, and hydrogen peroxide, as well as the antioxidant activity of superoxide dismutase, glutathione peroxidase, and catalase in washed erythrocyte and serum samples from full-term pregnant rats. For non-pregnant rats and other species, it is necessary to standardize these determinations, especially the sample volume. All measurements were normalized by the estimated protein concentrations in each sample. To establish optimum conditions for the reproducibility of the proposed methods, we describe all changes made in each assay's steps based on the reference method reassessed for the new standardizations. Furthermore, the calculations of the concentrations or activities of each marker are presented. Thus, we demonstrate that the analysis of serum samples is easier and faster, but it is impossible to detect catalase activity. Furthermore, the proposed methods can be applied for redox balance determination, especially using smaller reagent amounts and lower sample volumes in lesser time without losing accuracy, as is required in obtaining samples during rat pregnancy. Competing Interests: Competing interests The authors have no competing interests to declare. (Copyright © 2023 The Authors; This is an open access article under the CC BY license (https://creativecommons.org/licenses/by/4.0/).) |
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| فهرسة مساهمة: | Keywords: Biomarker; Lipid peroxidation; Oxidative stress; Pregnancy; Redox status |
| تواريخ الأحداث: | Date Created: 20230313 Latest Revision: 20230314 |
| رمز التحديث: | 20231215 |
| مُعرف محوري في PubMed: | PMC9993082 |
| DOI: | 10.21769/BioProtoc.4626 |
| PMID: | 36908641 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2331-8325 |
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| DOI: | 10.21769/BioProtoc.4626 |