دورية أكاديمية
أعرض في EDS

Anatoxins from benthic cyanobacteria responsible for dog mortalities in New Brunswick, Canada.

التفاصيل البيبلوغرافية
العنوان: Anatoxins from benthic cyanobacteria responsible for dog mortalities in New Brunswick, Canada.
المؤلفون: McCarron P; Biotoxin Metrology, National Research Council Canada, 1411 Oxford St., Halifax, N.S., Canada. Electronic address: pearse.mccarron@nrc-cnrc.gc.ca., Rafuse C; Biotoxin Metrology, National Research Council Canada, 1411 Oxford St., Halifax, N.S., Canada., Scott S; New Brunswick Provincial Veterinary Laboratory, Department of Agriculture, Fisheries and Aquaculture, 850 Lincoln Road, Fredericton, N.B., Canada., Lawrence J; Department of Biology, University of New Brunswick, Fredericton, N.B., Canada., Bruce MR; Canadian Rivers Institute, Department of Biology, University of New Brunswick, Fredericton, N.B., Canada., Douthwright E; Department of Environment and Local Government, Government of New Brunswick, 20 McGloin St., Fredericton, N.B., Canada., Murphy C; Aquatic and Crop Resource Development, National Research Council Canada, 1411 Oxford St., Halifax, N.S., Canada., Reith M; Aquatic and Crop Resource Development, National Research Council Canada, 1411 Oxford St., Halifax, N.S., Canada., Beach DG; Biotoxin Metrology, National Research Council Canada, 1411 Oxford St., Halifax, N.S., Canada.
المصدر: Toxicon : official journal of the International Society on Toxinology [Toxicon] 2023 May; Vol. 227, pp. 107086. Date of Electronic Publication: 2023 Mar 11.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Pergamon Press Country of Publication: England NLM ID: 1307333 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-3150 (Electronic) Linking ISSN: 00410101 NLM ISO Abbreviation: Toxicon Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Oxford ; New York : Pergamon Press, c1962-
مواضيع طبية MeSH: Bacterial Toxins*/toxicity , Bacterial Toxins*/analysis , Cyanobacteria*/chemistry, Dogs ; Animals ; New Brunswick ; RNA, Ribosomal, 16S/genetics ; Tropanes/toxicity ; Canada
مستخلص: In July 2018 three dogs died after visiting the Wolastoq (Saint John River) near Fredericton, New Brunswick, in Atlantic Canada. All showed signs of toxicosis, and necropsies revealed non-specific pulmonary edema and multiple microscopic brain hemorrhages. Liquid chromatography-high-resolution mass spectrometry (LC-HRMS) analysis of vomitus and stomach contents as well as water and biota from the mortality sites confirmed the presence of anatoxins (ATXs), a class of potent neurotoxic alkaloids. The highest levels were measured in a dried benthic cyanobacterial mat that two of the dogs had been eating before falling ill and in a vomitus sample collected from one of the dogs. Concentrations of 357 and 785 mg/kg for anatoxin-a and dihydroanatoxin-a, respectively, were measured in the vomitus. Known anatoxin-producing species of Microcoleus were tentatively identified using microscopy and confirmed by 16S rRNA gene sequencing. The ATX synthetase gene, anaC, was detected in the samples and isolates. The pathology and experimental results confirmed the role of ATXs in these dog mortalities. Further research is required to understand drivers for toxic cyanobacteria in the Wolastoq and to develop methodology for assessing occurrence.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Crown Copyright © 2023. Published by Elsevier Ltd. All rights reserved.)
فهرسة مساهمة: Keywords: Anatoxin; Cyanotoxins; Dog mortalities; LC–HRMS; Microcoleus
المشرفين على المادة: 80023A73NK (anatoxin a)
0 (Bacterial Toxins)
0 (RNA, Ribosomal, 16S)
0 (Tropanes)
تواريخ الأحداث: Date Created: 20230313 Date Completed: 20230418 Latest Revision: 20230418
رمز التحديث: 20240628
DOI: 10.1016/j.toxicon.2023.107086
PMID: 36914100
قاعدة البيانات: MEDLINE
الوصف
تدمد:1879-3150
DOI:10.1016/j.toxicon.2023.107086