دورية أكاديمية
أعرض في EDS

Large-scale expansion of human umbilical cord-derived mesenchymal stem cells using PLGA@PLL scaffold.

التفاصيل البيبلوغرافية
العنوان: Large-scale expansion of human umbilical cord-derived mesenchymal stem cells using PLGA@PLL scaffold.
المؤلفون: Liu Y; CAS Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, 510530 China.; Guangzhou Junyuankang Biotechnology Co., Ltd., Guangzhou, 510530 China., Amissah OB; CAS Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, 510530 China.; University of Chinese Academy of Sciences, Beijing, 100049 China., Huangfang X; School of Medicine, Guilin Medical University, Guilin, 541199 China., Wang L; School of Life Sciences, University of Science and Technology of China, Hefei, 230027 China., Dieu Habimana J; CAS Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, 510530 China.; University of Chinese Academy of Sciences, Beijing, 100049 China., Lv L; CAS Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, 510530 China.; University of Chinese Academy of Sciences, Beijing, 100049 China., Ding X; CAS Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, 510530 China.; University of Chinese Academy of Sciences, Beijing, 100049 China., Li J; CAS Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, 510530 China.; University of Chinese Academy of Sciences, Beijing, 100049 China., Chen M; School of Life Sciences, University of Science and Technology of China, Hefei, 230027 China., Zhu J; GZMU-GIBH Joint School of Life Sciences, Guangzhou Medical University, Guangzhou, 511436 China., Mukama O; CAS Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, 510530 China., Sun Y; CAS Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, 510530 China., Li Z; CAS Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, 510530 China.; University of Chinese Academy of Sciences, Beijing, 100049 China.; School of Life Sciences, University of Science and Technology of China, Hefei, 230027 China.; GZMU-GIBH Joint School of Life Sciences, Guangzhou Medical University, Guangzhou, 511436 China.; GIBH-HKU Guangdong-Hong Kong Stem Cell and Regenerative Medicine Research Centre, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, 510530 China.; Department of Anatomy and Neurobiology, Xiangya School of Medicine, Central South University, Changsha, 410013 China., Huang R; CAS Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, 510530 China.; GIBH-HKU Guangdong-Hong Kong Stem Cell and Regenerative Medicine Research Centre, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, 510530 China.
المصدر: Bioresources and bioprocessing [Bioresour Bioprocess] 2023; Vol. 10 (1), pp. 18. Date of Electronic Publication: 2023 Mar 08.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Springer-Verlag, GmbH Country of Publication: Germany NLM ID: 101665551 Publication Model: Print-Electronic Cited Medium: Print ISSN: 2197-4365 (Print) Linking ISSN: 21974365 NLM ISO Abbreviation: Bioresour Bioprocess Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Heidelberg, Germany : Springer-Verlag, GmbH, [2014]-
مستخلص: Mesenchymal stem cells (MSCs) are highly important in biomedicine and hold great potential in clinical treatment for various diseases. In recent years, the capabilities of MSCs have been under extensive investigation for practical application. Regarding therapy, the efficacy usually depends on the amount of MSCs. Nevertheless, the yield of MSCs is still limited due to the traditional cultural methods. Herein, we proposed a three-dimensional (3D) scaffold prepared using poly lactic-co-glycolic acid (PLGA) nanofiber with polylysine (PLL) grafting, to promote the growth and proliferation of MSCs derived from the human umbilical cord (hUC-MSCs). We found that the inoculated hUC-MSCs adhered efficiently to the PLGA scaffold with good affinity, fast growth rate, and good multipotency. The harvested cells were ideally distributed on the scaffold and we were able to gain a larger yield than the traditional culturing methods under the same condition. Thus, our cell seeding with a 3D scaffold could serve as a promising strategy for cell proliferation in the large-scale production of MSCs. Moreover, the simplicity and low preparation cost allow this 3D scaffold to extend its potential application beyond cell culture.
Supplementary Information: The online version contains supplementary material available at 10.1186/s40643-023-00635-6.
Competing Interests: Competing interestsThe authors declare no competing interests.
(© The Author(s) 2023.)
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فهرسة مساهمة: Keywords: Biomedicine; Large-scale expansion culture; MSCs; PLGA@PLL scaffold
تواريخ الأحداث: Date Created: 20230314 Latest Revision: 20230315
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC9994782
DOI: 10.1186/s40643-023-00635-6
PMID: 36915643
قاعدة البيانات: MEDLINE
الوصف
تدمد:2197-4365
DOI:10.1186/s40643-023-00635-6