دورية أكاديمية
Triap1 upregulation promotes escape from mitotic-slippage-induced G1 arrest.
| العنوان: | Triap1 upregulation promotes escape from mitotic-slippage-induced G1 arrest. |
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| المؤلفون: | Pavani M; IFOM ETS - The AIRC Institute of Molecular Oncology, Via Adamello 16, 20139 Milano, Italy. Electronic address: mattia.pavani@ifom.eu., Chiroli E; IFOM ETS - The AIRC Institute of Molecular Oncology, Via Adamello 16, 20139 Milano, Italy., Cancrini C; IFOM ETS - The AIRC Institute of Molecular Oncology, Via Adamello 16, 20139 Milano, Italy., Gross F; ImmunoConcEpT, CNRS UMR5164, Université de Bordeaux, 33076 Bordeaux, France., Bonaiuti P; IFOM ETS - The AIRC Institute of Molecular Oncology, Via Adamello 16, 20139 Milano, Italy., Villa S; Dipartimento di Biotecnologie Mediche e Medicina Traslazionale, Universitá degli Studi di Milano, 20090 Segrate, Italy., Giavazzi F; Dipartimento di Biotecnologie Mediche e Medicina Traslazionale, Universitá degli Studi di Milano, 20090 Segrate, Italy., Matafora V; IFOM ETS - The AIRC Institute of Molecular Oncology, Via Adamello 16, 20139 Milano, Italy., Bachi A; IFOM ETS - The AIRC Institute of Molecular Oncology, Via Adamello 16, 20139 Milano, Italy., Fava LL; Armenise-Harvard Laboratory of Cell Division, Department of Cellular, Computational and Integrative Biology - CIBIO, University of Trento, Trento, Italy., Lischetti T; IFOM ETS - The AIRC Institute of Molecular Oncology, Via Adamello 16, 20139 Milano, Italy. Electronic address: tiziana.lischetti@icloud.com., Ciliberto A; IFOM ETS - The AIRC Institute of Molecular Oncology, Via Adamello 16, 20139 Milano, Italy; Pázmány Péter Catholic University, Faculty of Information Technology and Bionics, 1083 Budapest, Hungary. Electronic address: andrea.ciliberto@ifom.eu. |
| المصدر: | Cell reports [Cell Rep] 2023 Mar 28; Vol. 42 (3), pp. 112215. Date of Electronic Publication: 2023 Mar 13. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Cell Press Country of Publication: United States NLM ID: 101573691 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2211-1247 (Electronic) NLM ISO Abbreviation: Cell Rep Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: [Cambridge, MA] : Cell Press, c 2012- |
| مواضيع طبية MeSH: | Mitosis* , Apoptosis*, Humans ; Nocodazole/pharmacology ; Up-Regulation ; Cell Proliferation ; G1 Phase ; Intracellular Signaling Peptides and Proteins/genetics |
| مستخلص: | Drugs targeting microtubules rely on the mitotic checkpoint to arrest cell proliferation. The prolonged mitotic arrest induced by such drugs is followed by a G1 arrest. Here, we follow for several weeks the fate of G1-arrested human cells after treatment with nocodazole. We find that a small fraction of cells escapes from the arrest and resumes proliferation. These escaping cells experience reduced DNA damage and p21 activation. Cells surviving treatment are enriched for anti-apoptotic proteins, including Triap1. Increasing Triap1 levels allows cells to survive the first treatment with reduced DNA damage and lower levels of p21; accordingly, decreasing Triap1 re-sensitizes cells to nocodazole. We show that Triap1 upregulation leads to the retention of cytochrome c in the mitochondria, opposing the partial activation of caspases caused by nocodazole. In summary, our results point to a potential role of Triap1 upregulation in the emergence of resistance to drugs that induce prolonged mitotic arrest. Competing Interests: Declaration of interests The authors declare no competing interests. (Copyright © 2023 The Author(s). Published by Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: CP: Cell biology; CP: Molecular biology; cell cycle; microtubule targeting drugs; mitosis; sublethal caspase activation |
| المشرفين على المادة: | SH1WY3R615 (Nocodazole) 0 (TRIAP1 protein, human) 0 (Intracellular Signaling Peptides and Proteins) |
| تواريخ الأحداث: | Date Created: 20230314 Date Completed: 20230403 Latest Revision: 20230410 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1016/j.celrep.2023.112215 |
| PMID: | 36917609 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2211-1247 |
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| DOI: | 10.1016/j.celrep.2023.112215 |