دورية أكاديمية
A mutant fitness assay identifies bacterial interactions in a model ocean hot spot.
| العنوان: | A mutant fitness assay identifies bacterial interactions in a model ocean hot spot. |
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| المؤلفون: | Schreier JE; Department of Marine Sciences, University of Georgia, Athens, GA 30602., Smith CB; Department of Marine Sciences, University of Georgia, Athens, GA 30602., Ioerger TR; Department of Computer Science and Engineering, Texas A&M University, College Station, TX 77843., Moran MA; Department of Marine Sciences, University of Georgia, Athens, GA 30602. |
| المصدر: | Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2023 Mar 21; Vol. 120 (12), pp. e2217200120. Date of Electronic Publication: 2023 Mar 15. |
| نوع المنشور: | Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: National Academy of Sciences Country of Publication: United States NLM ID: 7505876 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1091-6490 (Electronic) Linking ISSN: 00278424 NLM ISO Abbreviation: Proc Natl Acad Sci U S A Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Washington, DC : National Academy of Sciences |
| مواضيع طبية MeSH: | Seawater*/microbiology , Diatoms*/genetics, Phytoplankton/metabolism ; Base Sequence ; Oceans and Seas |
| مستخلص: | Bacteria that assemble in phycospheres surrounding living phytoplankton cells metabolize a substantial proportion of ocean primary productivity. Yet the type and extent of interactions occurring among species that colonize these micron-scale "hot spot" environments are challenging to study. We identified genes that mediate bacterial interactions in phycosphere communities by culturing a transposon mutant library of copiotrophic bacterium Ruegeria pomeroyi DSS-3 with the diatom Thalassiosira pseudonana CCMP1335 as the sole source of organic matter in the presence or absence of other heterotrophic bacterial species. The function of genes having significant effects on R. pomeroyi fitness indicated explicit cell-cell interactions initiated in the multibacterial phycospheres. We found that R. pomeroyi simultaneously competed for shared substrates while increasing reliance on substrates that did not support the other species' growth. Fitness outcomes also indicated that the bacterium competed for nitrogen in the forms of ammonium and amino acids; obtained purines, pyrimidines, and cofactors via crossfeeding; both initiated and defended antagonistic interactions; and sensed an environment with altered oxygen and superoxide levels. The large genomes characteristic of copiotrophic marine bacteria are hypothesized to enable responses to dynamic ecological challenges occurring at the scale of microns. Here, we discover >200 nonessential genes implicated in the management of fitness costs and benefits of membership in a globally significant bacterial community. |
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| معلومات مُعتمدة: | GRFP-1445117 National Science Foundation (NSF); OCE-2019589 National Science Foundation (NSF) |
| فهرسة مساهمة: | Keywords: Transposon Sequencing; bacteria; community ecology; phycosphere; phytoplankton |
| تواريخ الأحداث: | Date Created: 20230315 Date Completed: 20230320 Latest Revision: 20230408 |
| رمز التحديث: | 20231215 |
| مُعرف محوري في PubMed: | PMC10041152 |
| DOI: | 10.1073/pnas.2217200120 |
| PMID: | 36920927 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1091-6490 |
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| DOI: | 10.1073/pnas.2217200120 |