دورية أكاديمية
A mutant fitness assay identifies bacterial interactions in a model ocean hot spot.
العنوان: | A mutant fitness assay identifies bacterial interactions in a model ocean hot spot. |
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المؤلفون: | Schreier JE; Department of Marine Sciences, University of Georgia, Athens, GA 30602., Smith CB; Department of Marine Sciences, University of Georgia, Athens, GA 30602., Ioerger TR; Department of Computer Science and Engineering, Texas A&M University, College Station, TX 77843., Moran MA; Department of Marine Sciences, University of Georgia, Athens, GA 30602. |
المصدر: | Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2023 Mar 21; Vol. 120 (12), pp. e2217200120. Date of Electronic Publication: 2023 Mar 15. |
نوع المنشور: | Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, Non-U.S. Gov't |
اللغة: | English |
بيانات الدورية: | Publisher: National Academy of Sciences Country of Publication: United States NLM ID: 7505876 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1091-6490 (Electronic) Linking ISSN: 00278424 NLM ISO Abbreviation: Proc Natl Acad Sci U S A Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: Washington, DC : National Academy of Sciences |
مواضيع طبية MeSH: | Seawater*/microbiology , Diatoms*/genetics, Phytoplankton/metabolism ; Base Sequence ; Oceans and Seas |
مستخلص: | Bacteria that assemble in phycospheres surrounding living phytoplankton cells metabolize a substantial proportion of ocean primary productivity. Yet the type and extent of interactions occurring among species that colonize these micron-scale "hot spot" environments are challenging to study. We identified genes that mediate bacterial interactions in phycosphere communities by culturing a transposon mutant library of copiotrophic bacterium Ruegeria pomeroyi DSS-3 with the diatom Thalassiosira pseudonana CCMP1335 as the sole source of organic matter in the presence or absence of other heterotrophic bacterial species. The function of genes having significant effects on R. pomeroyi fitness indicated explicit cell-cell interactions initiated in the multibacterial phycospheres. We found that R. pomeroyi simultaneously competed for shared substrates while increasing reliance on substrates that did not support the other species' growth. Fitness outcomes also indicated that the bacterium competed for nitrogen in the forms of ammonium and amino acids; obtained purines, pyrimidines, and cofactors via crossfeeding; both initiated and defended antagonistic interactions; and sensed an environment with altered oxygen and superoxide levels. The large genomes characteristic of copiotrophic marine bacteria are hypothesized to enable responses to dynamic ecological challenges occurring at the scale of microns. Here, we discover >200 nonessential genes implicated in the management of fitness costs and benefits of membership in a globally significant bacterial community. |
References: | Environ Microbiol. 2005 Jun;7(6):860-73. (PMID: 15892705) Appl Environ Microbiol. 1999 Sep;65(9):3810-9. (PMID: 10473380) Ecol Lett. 2016 Jul;19(7):810-22. (PMID: 27282316) Science. 2013 Jun 7;340(6137):1223-6. (PMID: 23641059) ISME J. 2010 Jun;4(6):784-98. (PMID: 20072162) Evolution. 2014 Sep;68(9):2559-70. (PMID: 24910088) Proc Natl Acad Sci U S A. 2002 Dec 24;99(26):16684-8. (PMID: 12482933) Annu Rev Microbiol. 2003;57:155-76. (PMID: 12730324) Science. 2018 Aug 3;361(6401):469-474. (PMID: 30072533) Bioinformatics. 2009 Jul 15;25(14):1754-60. (PMID: 19451168) Nat Commun. 2018 Jan 18;9(1):266. (PMID: 29348571) Science. 2012 Nov 2;338(6107):628-33. (PMID: 23118182) mSystems. 2020 Aug 11;5(4):. (PMID: 32788406) Environ Microbiol Rep. 2011 Oct;3(5):535-42. (PMID: 23761332) Nat Microbiol. 2022 Apr;7(4):508-523. (PMID: 35365785) Proc Natl Acad Sci U S A. 2015 May 19;112(20):6449-54. (PMID: 25941371) Microbiol Spectr. 2021 Sep 3;9(1):e0026921. (PMID: 34190604) PLoS Comput Biol. 2015 Oct 08;11(10):e1004401. (PMID: 26447887) Sci Adv. 2022 Feb 25;8(8):eabk3076. (PMID: 35196097) ISME J. 2013 Feb;7(2):281-98. (PMID: 22931830) Mol Syst Biol. 2014 Aug 22;10:747. (PMID: 25149558) Science. 1987 Feb 6;235(4789):689-91. (PMID: 17833630) Sci Adv. 2021 Nov 05;7(45):eabi7159. (PMID: 34739314) Microbiol Mol Biol Rev. 2012 Sep;76(3):667-84. (PMID: 22933565) J Biol Chem. 2004 Feb 20;279(8):6613-9. (PMID: 14660605) mBio. 2019 Mar 12;10(2):. (PMID: 30862752) ISME J. 2019 Nov;13(11):2800-2816. (PMID: 31316134) Ann Rev Mar Sci. 2013;5:421-45. (PMID: 22881353) Nat Rev Microbiol. 2014 Oct;12(10):686-98. (PMID: 25134618) Nat Microbiol. 2016 Feb 29;1:16005. (PMID: 27572439) Glob Chang Biol. 2016 Aug;22(8):2633-50. (PMID: 27111095) Proc Natl Acad Sci U S A. 2020 Nov 3;117(44):27445-27455. (PMID: 33067398) Appl Environ Microbiol. 2000 Feb;66(2):578-87. (PMID: 10653721) ISME J. 2022 Sep;16(9):2198-2212. (PMID: 35739297) ISME J. 2015 Jun;9(6):1456-66. (PMID: 25575306) Environ Microbiol. 2018 Aug;20(8):2809-2823. (PMID: 29659156) Front Microbiol. 2018 Apr 06;9:659. (PMID: 29681892) Microbiol Resour Announc. 2020 Jul 23;9(30):. (PMID: 32703840) Nat Rev Microbiol. 2016 Feb;14(2):119-28. (PMID: 26775926) Biosci Biotechnol Biochem. 1997 Oct;61(10):1658-62. (PMID: 9362113) Proc Natl Acad Sci U S A. 2020 Feb 18;117(7):3656-3662. (PMID: 32015111) Proc Natl Acad Sci U S A. 2013 Oct 15;110(42):16754-9. (PMID: 24082144) Appl Environ Microbiol. 2020 Jun 17;86(13):. (PMID: 32332139) Appl Environ Microbiol. 2013 Jan;79(2):738-40. (PMID: 23144131) FEBS J. 2015 May;282(10):2029-44. (PMID: 25754869) Microbiol Spectr. 2014 Jun;2(3):. (PMID: 25346874) Appl Environ Microbiol. 2001 Nov;67(11):5343-8. (PMID: 11679368) ISME J. 2012 Jan;6(1):124-35. (PMID: 21776030) J Bacteriol. 2017 Aug 28;199(22):. (PMID: 28847922) ISME J. 2019 Nov;13(11):2868-2881. (PMID: 31358912) Science. 2022 May 6;376(6593):581-582. (PMID: 35511986) Nature. 2004 Dec 16;432(7019):910-3. (PMID: 15602564) Nat Prod Rep. 2018 May 1;35(5):455-488. (PMID: 29799048) Annu Rev Microbiol. 2006;60:255-80. (PMID: 16719716) Philos Trans R Soc Lond B Biol Sci. 2020 May 11;375(1798):20190706. (PMID: 32200734) ISME J. 2019 Oct;13(10):2536-2550. (PMID: 31227817) Nat Methods. 2009 Oct;6(10):767-72. (PMID: 19767758) Nat Rev Microbiol. 2007 Oct;5(10):782-91. (PMID: 17853906) Science. 2012 May 4;336(6081):608-11. (PMID: 22556258) Trends Microbiol. 2015 Sep;23(9):577-84. (PMID: 26051014) Methods Mol Biol. 2022;2377:391-421. (PMID: 34709629) J Plankton Res. 2018 Nov;40(6):655-666. (PMID: 30487658) Nat Microbiol. 2017 May 30;2:17065. (PMID: 28555622) Proc Natl Acad Sci U S A. 2012 Aug 28;109(35):14041-5. (PMID: 22826241) Environ Microbiol Rep. 2018 Aug;10(4):412-427. (PMID: 29411545) |
معلومات مُعتمدة: | GRFP-1445117 National Science Foundation (NSF); OCE-2019589 National Science Foundation (NSF) |
فهرسة مساهمة: | Keywords: Transposon Sequencing; bacteria; community ecology; phycosphere; phytoplankton |
تواريخ الأحداث: | Date Created: 20230315 Date Completed: 20230320 Latest Revision: 20230408 |
رمز التحديث: | 20231215 |
مُعرف محوري في PubMed: | PMC10041152 |
DOI: | 10.1073/pnas.2217200120 |
PMID: | 36920927 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1091-6490 |
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DOI: | 10.1073/pnas.2217200120 |