دورية أكاديمية
Experimental keratitis induced in rat by Acanthamoeba from distinct morphological groups/genotypes: a histological and immunohistochemical evaluation.
| العنوان: | Experimental keratitis induced in rat by Acanthamoeba from distinct morphological groups/genotypes: a histological and immunohistochemical evaluation. |
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| المؤلفون: | de Souza Fernandes N; Departamento de Análises Clínicas e Toxicológicas, Faculdade de Farmácia, Universidade Federal de Minas Gerais (UFMG), Minas Gerais, Belo Horizonte, Brazil., Caliari MV; Departamento de Patologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, Minas Gerais, Brazil., Oliveira FMS; Departamento de Patologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, Minas Gerais, Brazil., Neto ABC; Departamento de Patologia, Universidade Federal do Espírito Santo (UFES), Espírito Santo, Vitória, Brazil., Rodrigues IA; Departamento de Análises Clínicas e Toxicológicas, Faculdade de Farmácia, Universidade Federal de Minas Gerais (UFMG), Minas Gerais, Belo Horizonte, Brazil., Furst C; Santa Casa de Misericórdia, Belo Horizonte, Minas Gerais, Brazil., Costa AO; Departamento de Análises Clínicas e Toxicológicas, Faculdade de Farmácia, Universidade Federal de Minas Gerais (UFMG), Minas Gerais, Belo Horizonte, Brazil. adrincosta@gmail.com. |
| المصدر: | Parasitology research [Parasitol Res] 2023 May; Vol. 122 (5), pp. 1167-1175. Date of Electronic Publication: 2023 Mar 16. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Springer International Country of Publication: Germany NLM ID: 8703571 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1432-1955 (Electronic) Linking ISSN: 09320113 NLM ISO Abbreviation: Parasitol Res Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Berlin : Springer International, c1987- |
| مواضيع طبية MeSH: | Acanthamoeba*/genetics , Acanthamoeba Keratitis*/parasitology, Rats ; Humans ; Animals ; Rats, Wistar ; Cornea/parasitology ; Genotype ; Escherichia coli |
| مستخلص: | Species of the genus Acanthamoeba are free-living protozoans that occasionally act as parasites, causing a severe, progressive corneal infection termed Acanthamoeba keratitis (AK). The variable pathogenic potential among Acanthamoeba lineages has been shown by in vitro assays, but little is known about the behavior of different strains in animal models of AK. This work aimed to evaluate the infectivity of Acanthamoeba from distinct morphological groups and genotypes in a rat model of AK and apply an immunohistochemical technique for histological characterization of the lesions. Only a strain classified as group I/genotype T17, isolated from a soil source, caused ulcerated corneal lesions in two Wistar rats (n = 9) subjected to intrastromal inoculation. Two strains derived from AK human cases (group II/genotype T4 and group III/genotype T5) did not induce corneal lesions in the rats. A previous association of group II/genotype T4 trophozoites with lethally irradiated Escherichia coli did not influence the infectivity. A hyperimmune serum produced in Wistar rats was validated by an immunocytochemical technique using the three distinct strains and then applied for immunohistochemistry. The abundance of antigenic residues was observed in both corneas with keratitis, suggesting that the infectious process tended to resolve. Despite the low infection rate of the AK Wistar rat model, we produced an immunochemical tool with a potential diagnostic application. We also showed for the first time the ability of Acanthamoeba from T17 genotype to cause AK in experimental conditions. (© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.) |
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| معلومات مُعتمدة: | APQ-01100-14 Fundação de Amparo à Pesquisa do Estado de Minas Gerais-FAPEMIG |
| فهرسة مساهمة: | Keywords: Acanthamoeba keratitis; Immunohistochemistry; Rat cornea; Wistar rats |
| تواريخ الأحداث: | Date Created: 20230316 Date Completed: 20230414 Latest Revision: 20230414 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1007/s00436-023-07817-3 |
| PMID: | 36922408 |
| قاعدة البيانات: | MEDLINE |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 1432-1955 |
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| DOI: | 10.1007/s00436-023-07817-3 |