دورية أكاديمية
Ganoderma lucidum polysaccharides ameliorates D-galactose-induced aging salivary secretion disorders by upregulating the rhythm and aquaporins.
| العنوان: | Ganoderma lucidum polysaccharides ameliorates D-galactose-induced aging salivary secretion disorders by upregulating the rhythm and aquaporins. |
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| المؤلفون: | Wu M; Department of Anatomy, Laboratory of Neuroscience and Tissue Engineering, Basic Medical College, Chongqing Medical University, Chongqing 400016, China., Huang B; Department of Anatomy, Laboratory of Neuroscience and Tissue Engineering, Basic Medical College, Chongqing Medical University, Chongqing 400016, China., Hu L; Department of Stem Cell and Tissue Engineering, Basic Medical College, Chongqing Medical University, Chongqing 400016, China., Zhang T; Department of Anatomy, Chongqing Three Gorges Medical College, Chongqing 404120, China., Zhang B; Department of Anatomy, Laboratory of Neuroscience and Tissue Engineering, Basic Medical College, Chongqing Medical University, Chongqing 400016, China., Zhao X; Department of Anatomy, Laboratory of Neuroscience and Tissue Engineering, Basic Medical College, Chongqing Medical University, Chongqing 400016, China., Lu R; Department of Stem Cell and Tissue Engineering, Basic Medical College, Chongqing Medical University, Chongqing 400016, China., Xiong W; Department of Stem Cell and Tissue Engineering, Basic Medical College, Chongqing Medical University, Chongqing 400016, China; Department of Anatomy, Chongqing Three Gorges Medical College, Chongqing 404120, China; Chongqing Key Laboratory of Development and Utilization of Genuine Medicinal Materials in Three Gorges Reservoir Area, Chongqing 404100, China., Zhang S; Department of Anatomy, Laboratory of Neuroscience and Tissue Engineering, Basic Medical College, Chongqing Medical University, Chongqing 400016, China., Li J; Department of Stem Cell and Tissue Engineering, Basic Medical College, Chongqing Medical University, Chongqing 400016, China., Chen D; Department of Stem Cell and Tissue Engineering, Basic Medical College, Chongqing Medical University, Chongqing 400016, China; Department of Anatomy, Chongqing Three Gorges Medical College, Chongqing 404120, China; Chongqing Key Laboratory of Development and Utilization of Genuine Medicinal Materials in Three Gorges Reservoir Area, Chongqing 404100, China., Yang B; Department of Pharmacology, School of Basic Medical Sciences, State Key Laboratory of Natural and Biomimetic Drugs, Peking University, Beijing 100191, China., Li G; Department of Stem Cell and Tissue Engineering, Basic Medical College, Chongqing Medical University, Chongqing 400016, China; Department of Anatomy, Chongqing Three Gorges Medical College, Chongqing 404120, China; Chongqing Key Laboratory of Development and Utilization of Genuine Medicinal Materials in Three Gorges Reservoir Area, Chongqing 404100, China. Electronic address: liguoli929@163.com., Ran J; Department of Anatomy, Laboratory of Neuroscience and Tissue Engineering, Basic Medical College, Chongqing Medical University, Chongqing 400016, China; Chongqing Key Laboratory of Development and Utilization of Genuine Medicinal Materials in Three Gorges Reservoir Area, Chongqing 404100, China. Electronic address: ranjianhua@cqmu.edu.cn. |
| المصدر: | Experimental gerontology [Exp Gerontol] 2023 May; Vol. 175, pp. 112147. Date of Electronic Publication: 2023 Mar 28. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Science Country of Publication: England NLM ID: 0047061 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-6815 (Electronic) Linking ISSN: 05315565 NLM ISO Abbreviation: Exp Gerontol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Tarrytown Ny : Elsevier Science Original Publication: Oxford. |
| مواضيع طبية MeSH: | Salivation* , Reishi*/metabolism, Mice ; Animals ; Galactose ; Aquaporin 5/metabolism ; Aging ; Polysaccharides/pharmacology |
| مستخلص: | Longer-term deterioration in saliva secretion has been observed to occur in response to aging. The functional deterioration of the salivary gland damages swallowing and chewing abilities and consequently reduces life quality of the elderly. There are, however, only a few proven effective treatments for aging salivary secretion disorders. Ganoderma lucidum polysaccharide (GLP) has been applied to treat various diseases because of its safety, efficacy, and low cost. We investigated the protective effect of GLP on the submandibular gland (SMG) during aging. D-galactose (D-gal) was used to treat the aging mice, and the body weight, water consumption, saliva secretion, and flow rate were measured after 6 weeks of modeling. Micromorphological changes of the SMG were assessed by hematoxylin-eosin staining and transmission electron microscopy. RT-qPCR and Western blot were used to detect the expression of apoptotic proteins and inflammatory cytokines. Aquaporins (AQPs) and rhythmic protein expression were analyzed by immunohistochemistry and immunofluorescence. The results showed that GLP effectively promoted the expression of AQP5, AQP4, and AQP1, inhibited the release of TNF-α, IL-6, and Bax, and reduced inflammation and apoptosis. Further experiments showed that GLP promoted the up-regulation of core clock genes and proteins and restored the co-localized expression of CLOCK and AQP5 that were weakened during aging, helping to attenuate aging-induced weight loss, decreased salivation, and structural and functional damage. The findings of this work contribute to understanding the nature of age-related modifications in SMG by identifying changes in AQP5 expression and regulatory mechanisms linked to SMG dysfunction during aging. GLP is a potential drug for maintaining healthy salivary gland (SG) status and preventing SG deficiency in the elderly. Competing Interests: Conflict of interest The authors declare no conflict of interest. (Copyright © 2023. Published by Elsevier Inc.) |
| فهرسة مساهمة: | Keywords: Aging; Aquaporins; Ganoderma lucidum polysaccharide; Rhythm; Submandibular gland |
| المشرفين على المادة: | X2RN3Q8DNE (Galactose) 0 (Aquaporin 5) 0 (Polysaccharides) |
| تواريخ الأحداث: | Date Created: 20230316 Date Completed: 20230418 Latest Revision: 20230420 |
| رمز التحديث: | 20230421 |
| DOI: | 10.1016/j.exger.2023.112147 |
| PMID: | 36925084 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-6815 |
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| DOI: | 10.1016/j.exger.2023.112147 |