دورية أكاديمية
Phenolic-rich extract of Nopalea cochenillifera attenuates gastric lesions induced in experimental models through inhibiting oxidative stress, modulating inflammatory markers and a cytoprotective effect.
| العنوان: | Phenolic-rich extract of Nopalea cochenillifera attenuates gastric lesions induced in experimental models through inhibiting oxidative stress, modulating inflammatory markers and a cytoprotective effect. |
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| المؤلفون: | da Silva ECS; Graduate Program in Pharmaceutical Sciences, Federal University of Rio Grande do Norte (UFRN), Natal, Brazil. lailayec@yahoo.com.br., Bernardo Guerra GC; Graduate Program in Health Science, Federal University of Rio Grande do Norte, Natal, Brazil. edilanearaujo@hotmail.com.; Department of Biophysics and Pharmacology, Biosciences Center, Federal University of Rio Grande do Norte, Natal, Brazil. gerlaneguerra@hotmail.com., de Araújo ERD; Graduate Program in Pharmaceutical Sciences, Federal University of Rio Grande do Norte (UFRN), Natal, Brazil. lailayec@yahoo.com.br., Schlamb J; Cancer and Inflammation Research Laboratory, Morphology Department, Biosciences Center, Federal University of Rio Grande do Norte, Natal, Brazil. fernandes.araujo@ufrn.br.; Plants for Human Health Institute, North Carolina State University, 600 Laureate Way, Kannapolis, NC, 28081, USA. jmschlam@ncsu.edu., da Silva VC; Graduate Program in Drug Development and Technological Innovation, Federal University of Rio Grande do Norte, Natal, Brazil. vcs.biomed@gmail.com., de Aragão Tavares E; Graduate Program in Drug Development and Technological Innovation, Federal University of Rio Grande do Norte, Natal, Brazil. vcs.biomed@gmail.com., Dantas-Medeiros R; Graduate Program in Drug Development and Technological Innovation, Federal University of Rio Grande do Norte, Natal, Brazil. vcs.biomed@gmail.com., Abreu LS; Department of Organic Chemistry, Institute of Chemistry, Fluminense Federal University, Niterói, RJ, Brazil. abreu_lucas@id.uff.br., Fechine Tavares J; Federal University of Paraíba, João Pessoa, PB, Brazil. josean@ltf.ufpb.br., de Araújo Júnior RF; Cancer and Inflammation Research Laboratory, Morphology Department, Biosciences Center, Federal University of Rio Grande do Norte, Natal, Brazil. fernandes.araujo@ufrn.br., Esposito D; Plants for Human Health Institute, North Carolina State University, 600 Laureate Way, Kannapolis, NC, 28081, USA. jmschlam@ncsu.edu.; Department of Animal Science, NC State University, 120 Broughton Drive, Raleigh, North Carolina 27695, USA. daesposi@ncsu.edu., Moncada M; Plants for Human Health Institute, North Carolina State University, 600 Laureate Way, Kannapolis, NC, 28081, USA. jmschlam@ncsu.edu.; Department of Food, Bioprocessing and Nutrition Sciences, North Carolina State University, 400 Dan Allen Drive, Raleigh, NC, 27695, USA. mlmoncad@ncsu.edu., Maria Zucolotto S; Graduate Program in Pharmaceutical Sciences, Federal University of Rio Grande do Norte (UFRN), Natal, Brazil. lailayec@yahoo.com.br.; Graduate Program in Health Science, Federal University of Rio Grande do Norte, Natal, Brazil. edilanearaujo@hotmail.com.; Graduate Program in Drug Development and Technological Innovation, Federal University of Rio Grande do Norte, Natal, Brazil. vcs.biomed@gmail.com.; Research Group on Bioactive Natural Products, Department of Pharmacy, Federal University of Rio Grande do Norte, Natal, Brazil. szucolotto@hotmail.com.; Plants for Human Health Institute, North Carolina State University, 600 Laureate Way, Kannapolis, NC, 28081, USA. jmschlam@ncsu.edu. |
| المصدر: | Food & function [Food Funct] 2023 Apr 03; Vol. 14 (7), pp. 3242-3258. Date of Electronic Publication: 2023 Apr 03. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Royal Society of Chemistry Country of Publication: England NLM ID: 101549033 Publication Model: Electronic Cited Medium: Internet ISSN: 2042-650X (Electronic) Linking ISSN: 20426496 NLM ISO Abbreviation: Food Funct Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Cambridge : Royal Society of Chemistry |
| مواضيع طبية MeSH: | Stomach Ulcer*/chemically induced , Stomach Ulcer*/drug therapy , Stomach Ulcer*/pathology , Anti-Ulcer Agents*/chemistry , Cactaceae*, Rats ; Animals ; Plant Extracts/chemistry ; Rats, Wistar ; Ethanol/chemistry ; Indomethacin/adverse effects ; Oxidative Stress ; Models, Theoretical ; Gastric Mucosa/metabolism |
| مستخلص: | Nopalea cochenillifera (Cactaceae), popularly known as "palma" or "palma doce", is from Mexico, but it was widely introduced in Brazil through crops. It has been used as food and in traditional medicine and is a good source of phenolic compounds. In this study the phytochemical profile and gastroprotective activity of phenolic-rich extract of N. cochenillifera in acute gastric lesion models induced by ethanol and indomethacin were evaluated. High-performance liquid chromatography coupled with mass spectrometry (HPLC/ESI/MS n ) allowed the characterization of 12 compounds such as sugars, phenolics and flavonoids. Among polyphenols, the main peak was assigned to isorhamnetin-3- O -(2'',3''- O -di-rhamnose)-glucoside. The TPC and TFC in the dry extract were 67.85 mg of gallic acid equivalent per g/extract and 46.16 mg quercetin equivalent per g/extract, respectively. In the in vitro MTT assay, the extract showed no cytotoxicity and suppressed ROS levels in LPS-treated RAW 264.7 cells. Preclinical models in rats showed that a dose of 100 mg kg -1 ( p < 0.0001) in the ethanol model and doses of 100 mg kg -1 ( p < 0.5) and 200 mg kg -1 ( p < 0.01) in the indomethacin model reduced the gastric lesions. Also, the extract reduced the MPO, MDA, TNF-α and IL-1β levels and increased the GSH and IL-10 levels. The pre-treatment with the extract led to the upregulation of SOD and the downregulation of COX-2 by immunohistochemical analysis. It also showed a cytoprotective effect in the histopathological analysis and stimulated the restoration of the mucus content as observed in the periodic acid-Schiff analysis without modifying the pH, volume or total acidity of the gastric juice. Taken together, N. cochenillifera extract can be applied as a novel gastroprotective ingredient for food or pharmaceutical products. |
| المشرفين على المادة: | 0 (Plant Extracts) 0 (Anti-Ulcer Agents) 3K9958V90M (Ethanol) XXE1CET956 (Indomethacin) |
| تواريخ الأحداث: | Date Created: 20230317 Date Completed: 20230405 Latest Revision: 20230405 |
| رمز التحديث: | 20240829 |
| DOI: | 10.1039/d2fo03735a |
| PMID: | 36928439 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 2042-650X |
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| DOI: | 10.1039/d2fo03735a |