دورية أكاديمية
The meiotic LINC complex component KASH5 is an activating adaptor for cytoplasmic dynein.
| العنوان: | The meiotic LINC complex component KASH5 is an activating adaptor for cytoplasmic dynein. |
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| المؤلفون: | Garner KEL; School of Biological Sciences, Faculty of Biology, Medicine and Health, University of Manchester , Manchester, UK., Salter A; School of Biological Sciences, Faculty of Biology, Medicine and Health, University of Manchester , Manchester, UK.; A*STAR Institute of Medical Biology , Singapore, Singapore., Lau CK; MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge Biomedical Campus , Cambridge, UK., Gurusaran M; Wellcome Centre for Cell Biology, Institute of Cell Biology, University of Edinburgh , Edinburgh, UK., Villemant CM; School of Biological Sciences, Faculty of Biology, Medicine and Health, University of Manchester , Manchester, UK., Granger EP; School of Biological Sciences, Faculty of Biology, Medicine and Health, University of Manchester , Manchester, UK., McNee G; School of Biological Sciences, Faculty of Biology, Medicine and Health, University of Manchester , Manchester, UK., Woodman PG; School of Biological Sciences, Faculty of Biology, Medicine and Health, University of Manchester , Manchester, UK., Davies OR; Wellcome Centre for Cell Biology, Institute of Cell Biology, University of Edinburgh , Edinburgh, UK., Burke BE; A*STAR Institute of Medical Biology , Singapore, Singapore., Allan VJ; School of Biological Sciences, Faculty of Biology, Medicine and Health, University of Manchester , Manchester, UK.; A*STAR Institute of Medical Biology , Singapore, Singapore. |
| المصدر: | The Journal of cell biology [J Cell Biol] 2023 May 01; Vol. 222 (5). Date of Electronic Publication: 2023 Mar 22. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Rockefeller University Press Country of Publication: United States NLM ID: 0375356 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1540-8140 (Electronic) Linking ISSN: 00219525 NLM ISO Abbreviation: J Cell Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: New York : Rockefeller University Press |
| مواضيع طبية MeSH: | Cytoplasmic Dyneins*/genetics , Cytoplasmic Dyneins*/metabolism , Dynactin Complex*/genetics , Dynactin Complex*/metabolism , Microtubule-Associated Proteins*/genetics , Microtubule-Associated Proteins*/metabolism , Cell Cycle Proteins*/genetics , Cell Cycle Proteins*/metabolism, Animals ; Calcium/metabolism ; Mammals/metabolism ; Nuclear Envelope/genetics ; Nuclear Envelope/metabolism ; Telomere/metabolism |
| مستخلص: | Cytoplasmic dynein-driven movement of chromosomes during prophase I of mammalian meiosis is essential for synapsis and genetic exchange. Dynein connects to chromosome telomeres via KASH5 and SUN1 or SUN2, which together span the nuclear envelope. Here, we show that KASH5 promotes dynein motility in vitro, and cytosolic KASH5 inhibits dynein's interphase functions. KASH5 interacts with a dynein light intermediate chain (DYNC1LI1 or DYNC1LI2) via a conserved helix in the LIC C-terminal, and this region is also needed for dynein's recruitment to other cellular membranes. KASH5's N-terminal EF-hands are essential as the interaction with dynein is disrupted by mutation of key calcium-binding residues, although it is not regulated by cellular calcium levels. Dynein can be recruited to KASH5 at the nuclear envelope independently of dynactin, while LIS1 is essential for dynactin incorporation into the KASH5-dynein complex. Altogether, we show that the transmembrane protein KASH5 is an activating adaptor for dynein and shed light on the hierarchy of assembly of KASH5-dynein-dynactin complexes. (© 2023 Garner et al.) |
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| معلومات مُعتمدة: | United Kingdom WT_ Wellcome Trust; BB/N006933/1 United Kingdom BB_ Biotechnology and Biological Sciences Research Council; WT210711 United Kingdom WT_ Wellcome Trust; MC_UP_A025_1011 United Kingdom MRC_ Medical Research Council |
| المشرفين على المادة: | SY7Q814VUP (Calcium) EC 3.6.4.2 (Cytoplasmic Dyneins) 0 (Dynactin Complex) 0 (Microtubule-Associated Proteins) 0 (Cell Cycle Proteins) |
| تواريخ الأحداث: | Date Created: 20230322 Date Completed: 20230324 Latest Revision: 20240515 |
| رمز التحديث: | 20240515 |
| مُعرف محوري في PubMed: | PMC10071310 |
| DOI: | 10.1083/jcb.202204042 |
| PMID: | 36946995 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1540-8140 |
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| DOI: | 10.1083/jcb.202204042 |