دورية أكاديمية
Transfusion of Adult, but Not Neonatal, Platelets Promotes Monocyte Trafficking in Neonatal Mice.
| العنوان: | Transfusion of Adult, but Not Neonatal, Platelets Promotes Monocyte Trafficking in Neonatal Mice. |
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| المؤلفون: | Maurya P; Aab Cardiovascular Research Institute (P.M., S.K.T., C.L., C.N.M.), University of Rochester School of Medicine and Dentistry, NY.; Department of Pediatrics (P.M., K.M.S., K.E.M., J.P.), University of Rochester School of Medicine and Dentistry, NY., Ture SK; Aab Cardiovascular Research Institute (P.M., S.K.T., C.L., C.N.M.), University of Rochester School of Medicine and Dentistry, NY., Li C; Aab Cardiovascular Research Institute (P.M., S.K.T., C.L., C.N.M.), University of Rochester School of Medicine and Dentistry, NY., Scheible KM; Department of Pediatrics (P.M., K.M.S., K.E.M., J.P.), University of Rochester School of Medicine and Dentistry, NY., McGrath KE; Department of Pediatrics (P.M., K.M.S., K.E.M., J.P.), University of Rochester School of Medicine and Dentistry, NY., Palis J; Department of Pediatrics (P.M., K.M.S., K.E.M., J.P.), University of Rochester School of Medicine and Dentistry, NY.; Department of Pathology and Laboratory Medicine (J.P., C.N.M.), University of Rochester School of Medicine and Dentistry, NY., Morrell CN; Aab Cardiovascular Research Institute (P.M., S.K.T., C.L., C.N.M.), University of Rochester School of Medicine and Dentistry, NY.; Department of Medicine (C.N.M.), University of Rochester School of Medicine and Dentistry, NY.; Department of Microbiology and Immunology (C.N.M.), University of Rochester School of Medicine and Dentistry, NY.; Department of Pathology and Laboratory Medicine (J.P., C.N.M.), University of Rochester School of Medicine and Dentistry, NY. |
| المصدر: | Arteriosclerosis, thrombosis, and vascular biology [Arterioscler Thromb Vasc Biol] 2023 Jun; Vol. 43 (6), pp. 873-885. Date of Electronic Publication: 2023 Mar 23. |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural |
| اللغة: | English |
| بيانات الدورية: | Publisher: Lippincott Williams & Wilkins Country of Publication: United States NLM ID: 9505803 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1524-4636 (Electronic) Linking ISSN: 10795642 NLM ISO Abbreviation: Arterioscler Thromb Vasc Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 1998- : Baltimore, Md. : Lippincott Williams & Wilkins Original Publication: Dallas, TX : American Heart Association, c1995- |
| مواضيع طبية MeSH: | Monocytes* , Thrombocytopenia*/genetics, Mice ; Animals ; Animals, Newborn ; Blood Platelets ; Platelet Transfusion/adverse effects ; Platelet Transfusion/methods |
| مستخلص: | Background: Thrombocytopenia is common in preterm neonates. Platelet transfusions are sometimes given to thrombocytopenic neonates with the hope of reducing the bleeding risk, however, there are little clinical data to support this practice, and platelet transfusions may increase the bleeding risk or lead to adverse complications. Our group previously reported that fetal platelets expressed lower levels of immune-related mRNA compared with adult platelets. In this study, we focused on the effects of adult versus neonatal platelets on monocyte immune functions that may have an impact on neonatal immune function and transfusion complications. Methods: Using RNA sequencing of postnatal day 7 and adult platelets, we determined age-dependent platelet gene expression. Platelets and naive bone marrow-isolated monocytes were cocultured and monocyte phenotypes determined by RNA sequencing and flow cytometry. An in vivo model of platelet transfusion in neonatal thrombocytopenic mice was used in which platelet-deficient TPOR (thrombopoietin receptor) mutant mice were transfused with adult or postnatal day 7 platelets and monocyte phenotypes and trafficking were determined. Results: Adult and neonatal platelets had differential immune molecule expression, including Selp . Monocytes incubated with adult or neonatal mouse platelets had similar inflammatory (Ly6C hi ) but different trafficking phenotypes, as defined by CCR2 and CCR5 mRNA and surface expression. Blocking P-sel (P-selectin) interactions with its PSGL-1 (P-sel glycoprotein ligand-1) receptor on monocytes limited the adult platelet-induced monocyte trafficking phenotype, as well as adult platelet-induced monocyte migration in vitro. Similar results were seen in vivo, when thrombocytopenic neonatal mice were transfused with adult or postnatal day 7 platelets; adult platelets increased monocyte CCR2 and CCR5, as well as monocyte chemokine migration, whereas postnatal day 7 platelets did not. Conclusions: These data provide comparative insights into adult and neonatal platelet transfusion-regulated monocyte functions. The transfusion of adult platelets to neonatal mice was associated with an acute inflammatory and trafficking monocyte phenotype that was platelet P-sel dependent and may have an impact on complications associated with neonatal platelet transfusions. Competing Interests: Disclosures None. |
| التعليقات: | Comment in: Arterioscler Thromb Vasc Biol. 2023 Jun;43(6):886-888. (PMID: 37139838) |
| معلومات مُعتمدة: | R21 HL153409 United States HL NHLBI NIH HHS; R01 HL141106 United States HL NHLBI NIH HHS; R01 HL160610 United States HL NHLBI NIH HHS |
| فهرسة مساهمة: | Keywords: P-selectin; blood platelets; mice; monocytes; platelet transfusion |
| تواريخ الأحداث: | Date Created: 20230323 Date Completed: 20230526 Latest Revision: 20240528 |
| رمز التحديث: | 20240528 |
| DOI: | 10.1161/ATVBAHA.122.318162 |
| PMID: | 36951062 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1524-4636 |
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| DOI: | 10.1161/ATVBAHA.122.318162 |