دورية أكاديمية
CORP: Sources and degrees of variability in whole animal intermittent hypoxia experiments.
| العنوان: | CORP: Sources and degrees of variability in whole animal intermittent hypoxia experiments. |
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| المؤلفون: | Zhang Z; Interdisciplinary Graduate Program in Molecular Medicine, University of Iowa, Iowa City, Iowa, United States.; Division of Hematology, Oncology, and Bone Marrow Transplantation, Department of Internal Medicine, University of Iowa, Iowa City, Iowa, United States., Kalra H; Department of Health and Human Physiology, University of Iowa, Iowa City, Iowa, United States., Delzell MC; Department of Health and Human Physiology, University of Iowa, Iowa City, Iowa, United States.; Kirksville College of Osteopathic Medicine, A.T. Still University, Kirksville, Missouri, United States., Jedlicka CR; Department of Health and Human Physiology, University of Iowa, Iowa City, Iowa, United States., Vasilyev M; Division of Hematology, Oncology, and Bone Marrow Transplantation, Department of Internal Medicine, University of Iowa, Iowa City, Iowa, United States., Vasileva A; Department of Health and Human Physiology, University of Iowa, Iowa City, Iowa, United States., Tomasson MH; Interdisciplinary Graduate Program in Molecular Medicine, University of Iowa, Iowa City, Iowa, United States.; Division of Hematology, Oncology, and Bone Marrow Transplantation, Department of Internal Medicine, University of Iowa, Iowa City, Iowa, United States.; Department of Health and Human Physiology, University of Iowa, Iowa City, Iowa, United States., Bates ML; Interdisciplinary Graduate Program in Molecular Medicine, University of Iowa, Iowa City, Iowa, United States.; Division of Hematology, Oncology, and Bone Marrow Transplantation, Department of Internal Medicine, University of Iowa, Iowa City, Iowa, United States.; Department of Health and Human Physiology, University of Iowa, Iowa City, Iowa, United States.; Division of Neonatology, Department of Pediatrics, University of Iowa, Iowa City, Iowa, United States. |
| المصدر: | Journal of applied physiology (Bethesda, Md. : 1985) [J Appl Physiol (1985)] 2023 May 01; Vol. 134 (5), pp. 1207-1215. Date of Electronic Publication: 2023 Mar 23. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't; Research Support, N.I.H., Extramural |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Physiological Society Country of Publication: United States NLM ID: 8502536 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1522-1601 (Electronic) Linking ISSN: 01617567 NLM ISO Abbreviation: J Appl Physiol (1985) Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Bethesda, MD : American Physiological Society, c1985- |
| مواضيع طبية MeSH: | Hypoxia* , Oximetry*, Mice ; Animals ; Reproducibility of Results ; Disease Models, Animal ; Oxygen |
| مستخلص: | Chamber exposures are commonly used to evaluate the physiological and pathophysiological consequences of intermittent hypoxia in animal models. Researchers in this field use both commercial and custom-built chambers in their experiments. The purpose of this Cores of Reproducibility in Physiology paper is to demonstrate potential sources of variability in these systems that researchers should consider. Evaluating the relationship between arterial oxygen saturation and inspired oxygen concentration, we found that there are important sex-dependent differences in the commonly used C57BL6/J mouse model. The time delay of the oxygen sensor that provides feedback to the system during the ramp-down and ramp-up phases was different, limiting the number of cycles per hour that can be conducted and the overall stability of the oxygen concentration. The time to reach the hypoxic and normoxic hold stages, and the overall oxygen concentration, were impacted by the cycle number. These variables were further impacted by whether there are animals present in the chamber, highlighting the importance of verifying the cycling frequency with animals in the chamber. At ≤14 cycles/h, instability in the chamber oxygen concentration did not impact arterial oxygen saturation but may be important at higher cycle numbers. Taken together, these data demonstrate the important sources of variability that justify reporting and verifying the target oxygen concentration, cycling frequency, and arterial oxygen concentration, particularly when comparing different animal models and chamber configurations. NEW & NOTEWORTHY Intermittent hypoxia exposures are commonly used in physiology and many investigators use chamber systems to perform these studies. Because of the variety of chamber systems and protocols used, it is important to understand the sources of variability in intermittent hypoxia experiments that can impact reproducibility. We demonstrate sources of variability that come from the animal model, the intermittent hypoxia protocol, and the chamber system that can impact reproducibility. |
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| معلومات مُعتمدة: | R56 HL152365 United States HL NHLBI NIH HHS; R56HL152365 United States NH NIH HHS |
| فهرسة مساهمة: | Keywords: arterial oxygen saturation; chamber exposures; gas response kinetics; mouse models; sex as a biological variable |
| المشرفين على المادة: | S88TT14065 (Oxygen) |
| تواريخ الأحداث: | Date Created: 20230323 Date Completed: 20230427 Latest Revision: 20240502 |
| رمز التحديث: | 20240502 |
| مُعرف محوري في PubMed: | PMC10151045 |
| DOI: | 10.1152/japplphysiol.00643.2022 |
| PMID: | 36958346 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1522-1601 |
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| DOI: | 10.1152/japplphysiol.00643.2022 |