دورية أكاديمية
Evaluation of cold atmospheric plasma for the decontamination of flexible endoscopes.
| العنوان: | Evaluation of cold atmospheric plasma for the decontamination of flexible endoscopes. |
|---|---|
| المؤلفون: | Hervé RC; Environmental Healthcare Unit, School of Biological Sciences, University of Southampton, Southampton, UK. Electronic address: R.Herve@soton.ac.uk., Kong MG; Center for Bioelectrics, Old Dominion University, Norfolk, VA, USA., Bhatt S; Center for Bioelectrics, Old Dominion University, Norfolk, VA, USA., Chen HL; Center for Bioelectrics, Old Dominion University, Norfolk, VA, USA., Comoy EE; Commissariat à l'Energie Atomique et aux Energies Alternatives, Direction de la Recherche Fondamentale, Institut François Jacob, SEPIA, Université Paris-Saclay Fontenay-aux-Roses, France., Deslys JP; Commissariat à l'Energie Atomique et aux Energies Alternatives, Direction de la Recherche Fondamentale, Institut François Jacob, SEPIA, Université Paris-Saclay Fontenay-aux-Roses, France., Secker TJ; Environmental Healthcare Unit, School of Biological Sciences, University of Southampton, Southampton, UK., Keevil CW; Environmental Healthcare Unit, School of Biological Sciences, University of Southampton, Southampton, UK. |
| المصدر: | The Journal of hospital infection [J Hosp Infect] 2023 Jun; Vol. 136, pp. 100-109. Date of Electronic Publication: 2023 Mar 23. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: W.B. Saunders For The Hospital Infection Society Country of Publication: England NLM ID: 8007166 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1532-2939 (Electronic) Linking ISSN: 01956701 NLM ISO Abbreviation: J Hosp Infect Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: London : W.B. Saunders For The Hospital Infection Society Original Publication: New York, Academic Press. |
| مواضيع طبية MeSH: | Decontamination*/methods , Prions*, Animals ; Endoscopes |
| مستخلص: | Background: Despite adherence to standard protocols, residues including live micro-organisms may remain on the various surfaces of reprocessed flexible endoscopes. Prions are infectious proteins that are notoriously difficult to eliminate. Aim: To test the potential of cold atmospheric plasma (CAP) for the decontamination of various surfaces of flexible endoscopes, measuring total proteins and prion residual infectivity as indicators of efficacy. Methods: New PTFE endoscope channels and metal test surfaces spiked with test soil or prion-infected tissues were treated using different CAP-generating prototypes. Surfaces were examined for the presence of residues using very sensitive fluorescence epimicroscopy. Prion residual infectivity was determined using the wire implant animal model and a more sensitive cell infectivity assay. Findings: A CAP jet applied perpendicularly at close range on flat test surfaces removed soil within 3 min, but left microscopic residues and failed to eliminate prion infectivity according to the wire implant animal assay. The longitudinal gas flow from CAP prototypes developed for the treatment of long channels led to the displacement and sedimentation of residual soil towards the distal end, when applied alone. Observations of the plasma inside glass tubes showed temporal and spatial heterogeneity within a limited range. After the standard enzymatic manual pre-wash, 'CAP-activated' gas effluents prevented prion transmission from treated endoscope channels according to the prion infectivity cell assay. Conclusion: CAP shows promising results as a final step for decontamination of surgical surfaces. Optimizing CAP delivery could further enhance CAP efficacy, offering a safe, chemical-free alternative for the reprocessing of all luminal flexible endoscope surfaces. (Copyright © 2023 The Author(s). Published by Elsevier Ltd.. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Antimicrobial resistance; Biofilm; Cold atmospheric plasma; Decontamination; Luminal endoscopes; Proteins; Variant Creutzfeldt–Jakob disease |
| المشرفين على المادة: | 0 (Prions) |
| تواريخ الأحداث: | Date Created: 20230325 Date Completed: 20230605 Latest Revision: 20230605 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1016/j.jhin.2023.03.013 |
| PMID: | 36965823 |
| قاعدة البيانات: | MEDLINE |
Full Text via ClinicalKey 
Full Text Finder | تدمد: | 1532-2939 |
|---|---|
| DOI: | 10.1016/j.jhin.2023.03.013 |