دورية أكاديمية
Palmitoylation controls the stability of 190 kDa ankyrin-G in dendritic spines and is regulated by ZDHHC8 and lithium.
العنوان: | Palmitoylation controls the stability of 190 kDa ankyrin-G in dendritic spines and is regulated by ZDHHC8 and lithium. |
---|---|
المؤلفون: | Piguel NH; Department of Neuroscience, Northwestern University Feinberg School of Medicine, Chicago, IL, United States., Sanders SS; Shriners Hospitals Pediatric Research Center, Lewis Katz School of Medicine at Temple University, Philadelphia, PA, United States., De Simone FI; Shriners Hospitals Pediatric Research Center, Lewis Katz School of Medicine at Temple University, Philadelphia, PA, United States., Martin-de-Saavedra MD; Department of Neuroscience, Northwestern University Feinberg School of Medicine, Chicago, IL, United States.; Department of Biochemistry and Molecular Biology, School of Pharmacy, Instituto Universitario de Investigación en Neuroquímica, Complutense University of Madrid, Madrid, Spain., McCoig E; Department of Neuroscience, Northwestern University Feinberg School of Medicine, Chicago, IL, United States., Dionisio LE; Department of Neuroscience, Northwestern University Feinberg School of Medicine, Chicago, IL, United States., Smith KR; Department of Pharmacology, University of Colorado School of Medicine, Aurora, CO, United States., Thomas GM; Department of Anatomy and Cell Biology, Shriners Hospitals Pediatric Research Center, Lewis Katz School of Medicine at Temple University, Philadelphia, PA, United States., Penzes P; Department of Neuroscience, Northwestern University Feinberg School of Medicine, Chicago, IL, United States.; Department of Psychiatry and Behavioral Sciences, Northwestern University Feinberg School of Medicine, Chicago, IL, United States.; Northwestern University Center for Autism and Neurodevelopment, Chicago, IL, United States. |
المصدر: | Frontiers in molecular neuroscience [Front Mol Neurosci] 2023 Mar 08; Vol. 16, pp. 1144066. Date of Electronic Publication: 2023 Mar 08 (Print Publication: 2023). |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Frontiers Research Foundation Country of Publication: Switzerland NLM ID: 101477914 Publication Model: eCollection Cited Medium: Print ISSN: 1662-5099 (Print) Linking ISSN: 16625099 NLM ISO Abbreviation: Front Mol Neurosci Subsets: PubMed not MEDLINE |
أسماء مطبوعة: | Original Publication: Lausanne, Switzerland : Frontiers Research Foundation, 2008- |
مستخلص: | Introduction: AnkG, encoded by the ANK3 gene, is a multifunctional scaffold protein with complex isoform expression: the 480 and 270 kDa isoforms have roles at the axon initial segment and node of Ranvier, whereas the 190 kDa isoform (AnkG-190) has an emerging role in the dendritic shaft and spine heads. All isoforms of AnkG undergo palmitoylation, a post-translational modification regulating protein attachment to lipid membranes. However, palmitoylation of AnkG-190 has not been investigated in dendritic spines. The ANK3 gene and altered expression of AnkG proteins are associated with a variety of neuropsychiatric and neurodevelopmental disorders including bipolar disorder and are implicated in the lithium response, a commonly used mood stabilizer for bipolar disorder patients, although the precise mechanisms involved are unknown. Result: Here, we showed that Cys70 palmitoylation stabilizes the localization of AnkG-190 in spine heads and at dendritic plasma membrane nanodomains. Mutation of Cys70 impairs AnkG-190 function in dendritic spines and alters PSD-95 scaffolding. Interestingly, we find that lithium reduces AnkG-190 palmitoylation thereby increasing its mobility in dendritic spines. Finally, we demonstrate that the palmitoyl acyl transferase ZDHHC8, but not ZDHHC5, increases AnkG-190 stability in spine heads and is inhibited by lithium. Discussion: Together, our data reveal that palmitoylation is critical for AnkG-190 localization and function and a potential ZDHHC8/AnkG-190 mechanism linking AnkG-190 mobility to the neuronal effects of lithium. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2023 Piguel, Sanders, De Simone, Martin-de-Saavedra, McCoig, Dionisio, Smith, Thomas and Penzes.) |
References: | Neuron. 2014 Apr 16;82(2):430-43. (PMID: 24742464) J Neurosci. 2011 Aug 24;31(34):12129-38. (PMID: 21865455) Am J Med Genet B Neuropsychiatr Genet. 2012 Dec;159B(8):997-1005. (PMID: 23109352) Mol Psychiatry. 2021 Jun;26(6):1775-1789. (PMID: 33398084) J Cell Biol. 2014 Jul 21;206(2):273-88. (PMID: 25049274) Transl Psychiatry. 2018 Oct 8;8(1):210. (PMID: 30297702) Eur Neuropsychopharmacol. 2017 Mar;27(3):274-288. (PMID: 28109561) Curr Opin Cell Biol. 2018 Feb;50:64-71. (PMID: 29475137) Hum Mol Genet. 2013 May 15;22(10):1960-70. (PMID: 23390136) Sci Rep. 2016 Apr 05;6:23981. (PMID: 27046665) J Biol Chem. 2012 Dec 21;287(52):43995-4005. (PMID: 23129772) J Neurosci. 2013 Sep 25;33(39):15401-7. (PMID: 24068808) J Biol Chem. 2007 Jan 19;282(3):2029-37. (PMID: 17074766) Psychiatr Genet. 2017 Dec;27(6):225-235. (PMID: 29068871) Proc Natl Acad Sci U S A. 1996 Aug 6;93(16):8455-9. (PMID: 8710892) Mol Psychiatry. 2009 Aug;14(8):755-63. (PMID: 19488044) J Hum Genet. 2011 Nov;56(11):779-83. (PMID: 21866111) Angew Chem Int Ed Engl. 2011 Oct 10;50(42):9838-42. (PMID: 21905186) J Cell Sci. 2019 Jul 5;132(13):. (PMID: 31189538) Transl Psychiatry. 2016 Nov 29;6(11):e959. (PMID: 27898073) Br J Psychiatry. 2014 Sep;205(3):244-5. (PMID: 24809399) J Psychiatry Neurosci. 2021 Jun 02;46(3):E402-E414. (PMID: 34077150) J Biol Chem. 2007 Sep 7;282(36):26552-61. (PMID: 17620337) Mol Psychiatry. 2009 May;14(5):487-91. (PMID: 19088739) Neurosci Lett. 2005 Dec 30;390(3):166-70. (PMID: 16150541) Neuron. 2014 Oct 22;84(2):399-415. (PMID: 25374361) J Biol Chem. 2013 May 17;288(20):14018-14031. (PMID: 23530049) Am J Med Genet B Neuropsychiatr Genet. 2011 Dec;156B(8):969-74. (PMID: 21972176) Nature. 2008 Dec 18;456(7224):904-9. (PMID: 19092927) Sci Adv. 2015 Sep 11;1(8):e1500301. (PMID: 26523289) Biol Psychiatry. 2013 Apr 1;73(7):683-90. (PMID: 23237312) Proc Natl Acad Sci U S A. 2009 May 5;106(18):7501-6. (PMID: 19416921) Mol Psychiatry. 2013 Aug;18(8):922-9. (PMID: 22850628) Hum Mutat. 2012 Dec;33(12):1635-8. (PMID: 22865819) Mol Psychiatry. 2017 Oct;22(10):1464-1472. (PMID: 27956739) Proc Natl Acad Sci U S A. 2017 Sep 26;114(39):10479-10484. (PMID: 28894008) Cell. 2009 Apr 3;137(1):159-71. (PMID: 19345194) J Neurosci. 2014 Feb 5;34(6):2075-86. (PMID: 24501349) Nat Commun. 2014 Mar 11;5:3339. (PMID: 24618891) Acta Biochim Biophys Sin (Shanghai). 2020 Dec 11;52(11):1181-1190. (PMID: 33098288) Cell Death Dis. 2018 Jul 23;9(8):795. (PMID: 30038264) J Clin Psychiatry. 2002 Oct;63(10):942-7. (PMID: 12416605) Eur J Endocrinol. 2005 Dec;153(6):929-38. (PMID: 16322400) J Affect Disord. 2017 Aug 1;217:266-280. (PMID: 28437764) Proc Natl Acad Sci U S A. 2015 Jan 27;112(4):957-64. (PMID: 25552556) Semin Cell Dev Biol. 2014 Mar;27:44-51. (PMID: 24239676) Neuropsychopharmacology. 2022 Nov 14;:. (PMID: 36376465) Front Cell Neurosci. 2017 May 09;11:136. (PMID: 28536506) Neuron. 2012 Feb 9;73(3):482-96. (PMID: 22325201) Proc Natl Acad Sci U S A. 2015 Jan 27;112(4):1214-9. (PMID: 25552561) Cell Rep. 2019 Mar 19;26(12):3284-3297.e3. (PMID: 30893601) J Cell Biol. 2010 Dec 27;191(7):1229-38. (PMID: 21187327) J Cell Biol. 1998 Sep 21;142(6):1571-81. (PMID: 9744885) Lancet. 2016 Mar 12;387(10023):1085-1093. (PMID: 26806518) |
معلومات مُعتمدة: | R01 MH107182 United States MH NIMH NIH HHS |
فهرسة مساهمة: | Keywords: ANK3; ZDHHC8; ankyrin-G; dendrite; dendritic spine; lithium; palmitoylation |
تواريخ الأحداث: | Date Created: 20230327 Latest Revision: 20240224 |
رمز التحديث: | 20240224 |
مُعرف محوري في PubMed: | PMC10031057 |
DOI: | 10.3389/fnmol.2023.1144066 |
PMID: | 36969554 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1662-5099 |
---|---|
DOI: | 10.3389/fnmol.2023.1144066 |