دورية أكاديمية
Tiotropium Bromide Improves Neutrophilic Asthma by Recovering Histone Deacetylase 2 Activity.
العنوان: | Tiotropium Bromide Improves Neutrophilic Asthma by Recovering Histone Deacetylase 2 Activity. |
---|---|
المؤلفون: | An TJ; Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, Yeouido St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul, Korea., Kim JH; Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, Yeouido St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul, Korea., Hur J; Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul, Korea., Park CK; Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, Yeouido St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul, Korea., Lim JU; Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, Yeouido St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul, Korea., Kim S; Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, Yeouido St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul, Korea., Rhee CK; Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul, Korea., Yoon HK; Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, Yeouido St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul, Korea. cmcyhg@gmail.com. |
المصدر: | Journal of Korean medical science [J Korean Med Sci] 2023 Mar 27; Vol. 38 (12), pp. e91. Date of Electronic Publication: 2023 Mar 27. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Korean Academy of Medical Science Country of Publication: Korea (South) NLM ID: 8703518 Publication Model: Electronic Cited Medium: Internet ISSN: 1598-6357 (Electronic) Linking ISSN: 10118934 NLM ISO Abbreviation: J Korean Med Sci Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: Seoul, Korea : Korean Academy of Medical Science, [1986- |
مواضيع طبية MeSH: | Asthma*/drug therapy , Histone Deacetylase 2*/metabolism , Tiotropium Bromide*/pharmacology, Animals ; Humans ; Mice ; Inflammation ; Lipopolysaccharides ; Mice, Inbred C57BL ; NF-kappa B/metabolism |
مستخلص: | Background: The value of tiotropium bromide (TIO) in neutrophilic asthma was meaningful in previous study. We hypothesized that TIO's mechanism of action is associated with histone deacetylase 2 (HDAC2) activity, which is key for controlling the transcription of inflammatory cytokines and usually downregulated in neutrophilic asthma. Methods: The effects of TIO and dexamethasone (DEX) on HDAC2 activity, nuclear factor kappa B (NF-κB), and C-X-C motif chemokine ligand 1 (CXCL1) were evaluated in neutrophilic asthma mouse model (C57BL, 6-week-old). An in-vitro study was conducted using primary human bronchial/tracheal epithelial (HBE) cells from asthma patients. Western blot analyses were performed for phospho-phospholipase Cγ-1 (PLCγ-1) and inositol trisphosphate (IP Results: Ovalbumin was used to induce eosinophilic inflammation in this study. After neutrophilic asthma was induced by LPS (O+L group), HDAC2 activity was diminished with increased NF-κB activity and CXCL1 compared to the control group. TIO significantly improved NF-κB activity, CXCL1, and HDAC2 activity compared with the O+L group in in-vivo study ( P < 0.05, each). Western blot analyses showed that LPS treated HBE cells from asthma patients increased PLCγ-1 and diminished IP Conclusion: These results support the hypothesis that TIO modulates inflammation by recovering HDAC2 activity from the acetylcholine-stimulated inflammation cascade in neutrophilic asthma. The detailed inflammation cascade of recovering HDAC2 activity by TIO might be associated with PLCγ-1-IP Competing Interests: The authors have no potential conflicts of interest to disclose. (© 2023 The Korean Academy of Medical Sciences.) |
References: | PLoS One. 2013;8(2):e58027. (PMID: 23469136) Thorax. 2013 Apr;68(4):322-9. (PMID: 23291349) Physiol Rev. 2016 Oct;96(4):1261-96. (PMID: 27512009) Sci Rep. 2019 Aug 12;9(1):11631. (PMID: 31406171) Front Oncol. 2017 Jul 05;7:140. (PMID: 28725634) Eur Respir J. 2021 Dec 31;59(1):. (PMID: 34667060) Semin Immunol. 2017 Oct;33:65-73. (PMID: 29042030) Eur J Med Res. 2010 Nov 4;15 Suppl 2:64-7. (PMID: 21147623) Toxicol Appl Pharmacol. 2019 Dec 1;384:114778. (PMID: 31618660) J Exp Med. 2006 Jan 23;203(1):7-13. (PMID: 16380507) Chest. 2020 Jan;157(1):26-33. (PMID: 31525357) Expert Opin Pharmacother. 2017 Feb;18(3):305-312. (PMID: 28110558) Pulm Pharmacol Ther. 2010 Aug;23(4):345-54. (PMID: 20362689) Ther Clin Risk Manag. 2015 Oct 08;11:1563-72. (PMID: 26504398) Allergy Asthma Immunol Res. 2022 Jan;14(1):1-4. (PMID: 34983102) ERJ Open Res. 2019 Dec 23;5(4):. (PMID: 31886156) Respir Res. 2016 Nov 16;17(1):153. (PMID: 27852250) Clin Exp Pharmacol Physiol. 2022 Jun;49(6):624-632. (PMID: 35181901) Eur Respir J. 2019 Nov 7;54(5):. (PMID: 31439682) Clin Exp Allergy. 2014 Jan;44(1):47-57. (PMID: 24355018) Tuberc Respir Dis (Seoul). 2018 Jan;81(1):80-87. (PMID: 29332324) Lancet Respir Med. 2021 May;9(5):441-443. (PMID: 33577751) Chest. 2014 Feb;145(2):305-312. (PMID: 24030221) Life Sci. 2016 May 1;152:107-16. (PMID: 27038884) J Allergy Clin Immunol. 2013 Dec;132(6):1295-302. (PMID: 23998657) Eur Respir J. 2019 Nov 7;54(5):. (PMID: 31699782) Trends Immunol. 2017 Dec;38(12):942-954. (PMID: 28784414) Ther Adv Respir Dis. 2009 Oct;3(5):235-43. (PMID: 19812111) Thorax. 2012 May;67(5):392-8. (PMID: 22156779) ERJ Open Res. 2016 Oct 26;2(4):. (PMID: 28053970) Am J Physiol Lung Cell Mol Physiol. 2019 Jan 1;316(1):L269-L279. (PMID: 30407865) Tuberc Respir Dis (Seoul). 2022 Jan;85(1):18-24. (PMID: 34727490) |
معلومات مُعتمدة: | Korea Korean Academy of Tuberculosis and Respiratory Diseases |
فهرسة مساهمة: | Keywords: Asthma; Histone Deacetylases 2; Neutrophils; Tiotropium bromide |
المشرفين على المادة: | EC 3.5.1.98 (Histone Deacetylase 2) 0 (Lipopolysaccharides) 0 (NF-kappa B) XX112XZP0J (Tiotropium Bromide) |
تواريخ الأحداث: | Date Created: 20230328 Date Completed: 20230405 Latest Revision: 20230405 |
رمز التحديث: | 20240628 |
مُعرف محوري في PubMed: | PMC10042725 |
DOI: | 10.3346/jkms.2023.38.e91 |
PMID: | 36974400 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1598-6357 |
---|---|
DOI: | 10.3346/jkms.2023.38.e91 |