دورية أكاديمية
أعرض في EDS

Susceptibility of Ovine Bone Marrow-Derived Mesenchymal Stem Cell Spheroids to Scrapie Prion Infection.

التفاصيل البيبلوغرافية
العنوان: Susceptibility of Ovine Bone Marrow-Derived Mesenchymal Stem Cell Spheroids to Scrapie Prion Infection.
المؤلفون: Hernaiz A; Laboratorio de Genética Bioquímica (LAGENBIO), Facultad de Veterinaria, Universidad de Zaragoza, IA2, IIS-Aragón, 50013 Zaragoza, Spain., Cobeta P; Laboratorio de Genética Bioquímica (LAGENBIO), Facultad de Veterinaria, Universidad de Zaragoza, IA2, IIS-Aragón, 50013 Zaragoza, Spain., Marín B; Centro de Encefalopatías y Enfermedades Transmisibles Emergentes (CEETE), Facultad de Veterinaria, Universidad de Zaragoza, IA2, IIS-Aragón, 50013 Zaragoza, Spain., Vázquez FJ; Laboratorio de Genética Bioquímica (LAGENBIO), Facultad de Veterinaria, Universidad de Zaragoza, IA2, IIS-Aragón, 50013 Zaragoza, Spain.; Equine Surgery and Medicine Service, Veterinary Hospital (HVUZ), Universidad de Zaragoza, 50013 Zaragoza, Spain., Badiola JJ; Centro de Encefalopatías y Enfermedades Transmisibles Emergentes (CEETE), Facultad de Veterinaria, Universidad de Zaragoza, IA2, IIS-Aragón, 50013 Zaragoza, Spain., Zaragoza P; Laboratorio de Genética Bioquímica (LAGENBIO), Facultad de Veterinaria, Universidad de Zaragoza, IA2, IIS-Aragón, 50013 Zaragoza, Spain.; Departamento de Patología Animal, Facultad de Veterinaria, Universidad de Zaragoza, 50013 Zaragoza, Spain., Bolea R; Centro de Encefalopatías y Enfermedades Transmisibles Emergentes (CEETE), Facultad de Veterinaria, Universidad de Zaragoza, IA2, IIS-Aragón, 50013 Zaragoza, Spain., Martín-Burriel I; Laboratorio de Genética Bioquímica (LAGENBIO), Facultad de Veterinaria, Universidad de Zaragoza, IA2, IIS-Aragón, 50013 Zaragoza, Spain.; Centro de Encefalopatías y Enfermedades Transmisibles Emergentes (CEETE), Facultad de Veterinaria, Universidad de Zaragoza, IA2, IIS-Aragón, 50013 Zaragoza, Spain.; Centro de Investigación Biomédica en Red de Enfermedades Neurodegenerativas (CIBERNED), Instituto de Salud Carlos III, 28029 Madrid, Spain.
المصدر: Animals : an open access journal from MDPI [Animals (Basel)] 2023 Mar 13; Vol. 13 (6). Date of Electronic Publication: 2023 Mar 13.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Molecular Diversity Preservation International Country of Publication: Switzerland NLM ID: 101635614 Publication Model: Electronic Cited Medium: Print ISSN: 2076-2615 (Print) Linking ISSN: 20762615 NLM ISO Abbreviation: Animals (Basel) Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : Molecular Diversity Preservation International, 2011-
مستخلص: In neurodegenerative diseases, including prion diseases, cellular in vitro models appear as fundamental tools for the study of pathogenic mechanisms and potential therapeutic compounds. Two-dimensional (2D) monolayer cell culture systems are the most used cell-based assays, but these platforms are not able to reproduce the microenvironment of in vivo cells. This limitation can be surpassed using three-dimensional (3D) culture systems such as spheroids that more effectively mimic in vivo cell interactions. Herein, we evaluated the effect of scrapie prion infection in monolayer-cultured ovine bone marrow-derived mesenchymal stem cells (oBM-MSCs) and oBM-MSC-derived spheroids in growth and neurogenic conditions, analyzing their cell viability and their ability to maintain prion infection. An MTT assay was performed in oBM-MSCs and spheroids subjected to three conditions: inoculated with brain homogenate from scrapie-infected sheep, inoculated with brain homogenate from healthy sheep, and non-inoculated controls. The 3D conditions improved the cell viability in most cases, although in scrapie-infected spheroids in growth conditions, a decrease in cell viability was observed. The levels of pathological prion protein (PrP Sc ) in scrapie-infected oBM-MSCs and spheroids were measured by ELISA. In neurogenic conditions, monolayer cells and spheroids maintained the levels of PrP Sc over time. In growth conditions, however, oBM-MSCs showed decreasing levels of PrP Sc throughout time, whereas spheroids were able to maintain stable PrP Sc levels. The presence of PrP Sc in spheroids was also confirmed by immunocytochemistry. Altogether, these results show that a 3D culture microenvironment improves the permissiveness of oBM-MSCs to scrapie infection in growth conditions and maintains the infection ability in neurogenic conditions, making this model of potential use for prion studies.
Competing Interests: The authors declare no conflicts of interest.
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معلومات مُعتمدة: RTI2018-098711-B-100 Agencia Estatal de Investigación and Fondos FEDER; Consolidated research group A19-20R Gobierno de Aragón; predoctoral grant Order IIU/2023/2017 Gobierno de Aragón; predoctoral grant Order IIU/2023/2017 European Social Funds
فهرسة مساهمة: Keywords: 3D culture; mesenchymal stem cells; prion; scrapie; spheroids
تواريخ الأحداث: Date Created: 20230329 Latest Revision: 20230331
رمز التحديث: 20230331
مُعرف محوري في PubMed: PMC10044354
DOI: 10.3390/ani13061043
PMID: 36978584
قاعدة البيانات: MEDLINE
الوصف
تدمد:2076-2615
DOI:10.3390/ani13061043