دورية أكاديمية
أعرض في EDS

Enzyme-Regulated In Situ Formation of Copper Hexacyanoferrate Nanoparticles with Oxidase-Mimetic Behaviour for Colorimetric Detection of Ascorbate Oxidase.

التفاصيل البيبلوغرافية
العنوان: Enzyme-Regulated In Situ Formation of Copper Hexacyanoferrate Nanoparticles with Oxidase-Mimetic Behaviour for Colorimetric Detection of Ascorbate Oxidase.
المؤلفون: Zhang H; Chongqing Key Laboratory of High Active Traditional Chinese Drug Delivery System, Chongqing Medical and Pharmaceutical College, Chongqing 401331, China., Yang DN; Chongqing Key Laboratory of High Active Traditional Chinese Drug Delivery System, Chongqing Medical and Pharmaceutical College, Chongqing 401331, China., Li Y; Chongqing Key Laboratory of High Active Traditional Chinese Drug Delivery System, Chongqing Medical and Pharmaceutical College, Chongqing 401331, China., Yang FQ; School of Chemistry and Chemical Engineering, Chongqing University, Chongqing 401331, China.
المصدر: Biosensors [Biosensors (Basel)] 2023 Mar 04; Vol. 13 (3). Date of Electronic Publication: 2023 Mar 04.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI Pub Country of Publication: Switzerland NLM ID: 101609191 Publication Model: Electronic Cited Medium: Internet ISSN: 2079-6374 (Electronic) Linking ISSN: 20796374 NLM ISO Abbreviation: Biosensors (Basel) Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI Pub.
مواضيع طبية MeSH: Oxidoreductases* , Nanoparticles*, Humans ; Ascorbate Oxidase ; Copper ; Colorimetry/methods ; Ascorbic Acid ; Limit of Detection
مستخلص: In this study, a copper hexacyanoferrate nanoparticle with excellent oxidase-mimetic behaviour has been synthesized through a simple precipitation method. The synthesized copper hexacyanoferrate nanoparticle has intrinsic oxidase-like activity, which can catalyze the chromogenic reaction of 2,2'-azinobis-(3-ethylbenzthiazoline-6-sulphonate) through an O 2 •- reactive oxygen-species-participated process. On the other hand, K 3 [Fe(CN) 6 ] can be reduced by ascorbic acid (AA) to produce K 4 [Fe(CN) 6 ], thereby inhibiting the formation of the copper hexacyanoferrate nanoparticles. Furthermore, ascorbate oxidase (AAO) can catalyze the oxidation of AA to produce dehydroascorbic acid, which cannot reduce K 3 [Fe(CN) 6 ]. Thus, a system for an AAO-regulated in situ formation of copper hexacyanoferrate nanoparticles was constructed by coupling a prepared copper hexacyanoferrate nanozyme with AA for the detection of AAO activity. This colorimetric sensing assay shows high sensitivity and selectivity for the detection of AAO activity (the limit of detection is 0.52 U/L) with a linear range of 1.1-35.7 U/L. Finally, the developed method was applied to detect the activity of AAO in normal human serum with a satisfactory sample spiked recovery (87.4-108.8%). In short, this study provides a good strategy for the construction of nanozyme-based multi-enzyme cascade-signal amplification assay.
References: Spectrochim Acta A Mol Biomol Spectrosc. 2022 Nov 5;280:121564. (PMID: 35797885)
J Plant Physiol. 2007 Jan;164(1):39-46. (PMID: 16343690)
Anal Chim Acta. 2022 May 1;1205:339717. (PMID: 35414394)
J Pharm Biomed Anal. 2008 Feb 13;46(3):528-33. (PMID: 18082351)
Anal Chim Acta. 2021 Feb 8;1145:46-51. (PMID: 33453880)
Nat Nanotechnol. 2007 Sep;2(9):577-83. (PMID: 18654371)
Mikrochim Acta. 2019 Jun 24;186(7):466. (PMID: 31236752)
J Agric Food Chem. 2014 Jun 25;62(25):5827-34. (PMID: 24885936)
Chem Soc Rev. 2019 Jul 15;48(14):3683-3704. (PMID: 31119258)
J Am Chem Soc. 2011 Aug 3;133(30):11795-801. (PMID: 21692446)
Talanta. 2021 Jan 15;222:121522. (PMID: 33167232)
Anal Chim Acta. 2020 Apr 8;1105:162-168. (PMID: 32138915)
Anal Chem. 2019 Dec 17;91(24):15477-15483. (PMID: 31756070)
Anal Chim Acta. 2019 Dec 24;1091:59-68. (PMID: 31679575)
Chem Soc Rev. 2013 Jul 21;42(14):6060-93. (PMID: 23740388)
Mikrochim Acta. 2018 Aug 22;185(9):426. (PMID: 30136157)
Mikrochim Acta. 2019 Mar 16;186(4):246. (PMID: 30879229)
Talanta. 2022 Sep 1;247:123546. (PMID: 35594834)
Anal Bioanal Chem. 2021 Nov;413(26):6627-6637. (PMID: 34476525)
J Am Chem Soc. 2018 Sep 12;140(36):11302-11307. (PMID: 30118222)
Analyst. 2020 Feb 3;145(3):1001-1007. (PMID: 31830153)
معلومات مُعتمدة: CSTB2022NSCQ-BHX0687 Chongqing Postdoctoral Science Foundation, China; 2023MSXM006 Chongqing Medical Scientific Research Project (Joint project of Chongqing Health Commission and Science and Technology Bureau), China; YGZ2021133 Chongqing Medical and Pharmaceutical College, China
فهرسة مساهمة: Keywords: ascorbate oxidase; ascorbic acid; colorimetric detection; copper hexacyanoferrate nanoparticles; oxidase-like activity
المشرفين على المادة: EC 1.- (Oxidoreductases)
EC 1.10.3.3 (Ascorbate Oxidase)
FLX0VIC39Y (hexacyanoferrate II)
789U1901C5 (Copper)
PQ6CK8PD0R (Ascorbic Acid)
تواريخ الأحداث: Date Created: 20230329 Date Completed: 20230330 Latest Revision: 20230331
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC10046506
DOI: 10.3390/bios13030344
PMID: 36979556
قاعدة البيانات: MEDLINE
الوصف
تدمد:2079-6374
DOI:10.3390/bios13030344