دورية أكاديمية
أعرض في EDS

NOTCH1: A Novel Player in the Molecular Crosstalk Underlying Articular Chondrocyte Protection by Oleuropein and Hydroxytyrosol.

التفاصيل البيبلوغرافية
العنوان: NOTCH1: A Novel Player in the Molecular Crosstalk Underlying Articular Chondrocyte Protection by Oleuropein and Hydroxytyrosol.
المؤلفون: Panichi V; Laboratorio di Immunoreumatologia e Rigenerazione Tissutale, Laboratorio di Patologia delle Infezioni Associate all'Impianto, IRCCS Istituto Ortopedico Rizzoli, 40136 Bologna, Italy., Bissoli I; Dipartimento di Scienze Biomediche e Neuromotorie, Università di Bologna, 40138 Bologna, Italy., D'Adamo S; Dipartimento di Scienze Biomediche e Neuromotorie, Università di Bologna, 40138 Bologna, Italy., Flamigni F; Dipartimento di Scienze Biomediche e Neuromotorie, Università di Bologna, 40138 Bologna, Italy., Cetrullo S; Dipartimento di Scienze Biomediche e Neuromotorie, Università di Bologna, 40138 Bologna, Italy., Borzì RM; Laboratorio di Immunoreumatologia e Rigenerazione Tissutale, IRCCS Istituto Ortopedico Rizzoli, 40136 Bologna, Italy.
المصدر: International journal of molecular sciences [Int J Mol Sci] 2023 Mar 18; Vol. 24 (6). Date of Electronic Publication: 2023 Mar 18.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI, [2000-
مواضيع طبية MeSH: Osteoarthritis*/metabolism , Cartilage, Articular*/metabolism, Humans ; Chondrocytes/metabolism ; Lipopolysaccharides/pharmacology ; Cells, Cultured ; Receptor, Notch1/genetics ; Receptor, Notch1/metabolism
مستخلص: Osteoarthritis (OA) is the most common joint disease, but no effective and safe disease-modifying treatment is available. Risk factors such as age, sex, genetics, injuries and obesity can concur to the onset of the disease, variably triggering the loss of maturational arrest of chondrocytes further sustained by oxidative stress, inflammation and catabolism. Different types of nutraceuticals have been studied for their anti-oxidative and anti-inflammatory properties. Olive-derived polyphenols draw particular interest due to their ability to dampen the activation of pivotal signaling pathways in OA. Our study aims to investigate the effects of oleuropein (OE) and hydroxytyrosol (HT) in in vitro OA models and elucidate their possible effects on NOTCH1, a novel therapeutic target for OA. Chondrocytes were cultured and exposed to lipopolysaccharide (LPS). Detailed analysis was carried out about the OE/HT mitigating effects on the release of ROS (DCHF-DA), the increased gene expression of catabolic and inflammatory markers (real time RT-PCR), the release of MMP-13 (ELISA and Western blot) and the activation of underlying signaling pathways (Western blot). Our findings show that HT/OE efficiently attenuates LPS-induced effects by firstly reducing the activation of JNK and of the NOTCH1 pathway downstream. In conclusion, our study provides molecular bases supporting the dietary supplementation of olive-derived polyphenols to revert/delay the progression of OA.
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معلومات مُعتمدة: Fondi Cinque per Mille Anno 2019 Ministero della Salute; RFO University of Bologna; 2021 Fondazione Carisbo
فهرسة مساهمة: Keywords: LPS; MMP-13; NOTCH1; chondrocytes; inflammation; nutraceuticals; osteoarthritis; oxidative stress
المشرفين على المادة: 2O4553545L (oleuropein)
10597-60-1 (3,4-dihydroxyphenylethanol)
0 (Lipopolysaccharides)
0 (NOTCH1 protein, human)
0 (Receptor, Notch1)
تواريخ الأحداث: Date Created: 20230329 Date Completed: 20230330 Latest Revision: 20230405
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC10058228
DOI: 10.3390/ijms24065830
PMID: 36982904
قاعدة البيانات: MEDLINE
الوصف
تدمد:1422-0067
DOI:10.3390/ijms24065830