دورية أكاديمية
أعرض في EDS

Effect of ZEB1 Associated with microRNAs on Tumor Stem Cells in Head and Neck Cancer.

التفاصيل البيبلوغرافية
العنوان: Effect of ZEB1 Associated with microRNAs on Tumor Stem Cells in Head and Neck Cancer.
المؤلفون: Ferreira LAM; Genetics and Molecular Biology Research Unit (UPGEM), Medical School of São José do Rio Preto (FAMERP), São José do Rio Preto 15090-000, São Paulo, Brazil., Bezerra MADS; Genetics and Molecular Biology Research Unit (UPGEM), Medical School of São José do Rio Preto (FAMERP), São José do Rio Preto 15090-000, São Paulo, Brazil., Kawasaki-Oyama RS; Genetics and Molecular Biology Research Unit (UPGEM), Medical School of São José do Rio Preto (FAMERP), São José do Rio Preto 15090-000, São Paulo, Brazil., Fernandes GMM; Genetics and Molecular Biology Research Unit (UPGEM), Medical School of São José do Rio Preto (FAMERP), São José do Rio Preto 15090-000, São Paulo, Brazil., Castanhole-Nunes MMU; Genetics and Molecular Biology Research Unit (UPGEM), Medical School of São José do Rio Preto (FAMERP), São José do Rio Preto 15090-000, São Paulo, Brazil., Serafim Junior V; Genetics and Molecular Biology Research Unit (UPGEM), Medical School of São José do Rio Preto (FAMERP), São José do Rio Preto 15090-000, São Paulo, Brazil., Castilho RM; Laboratory of Epithelial Biology, Department of Periodontics and Oral Medicine, University of Michigan School of Dentistry, Ann Arbor, MI 48109, USA., Pavarino ÉC; Genetics and Molecular Biology Research Unit (UPGEM), Medical School of São José do Rio Preto (FAMERP), São José do Rio Preto 15090-000, São Paulo, Brazil., Maniglia JV; Department of Otolaryngology and Head and Neck Surgery, Medical School of São José do Rio Preto (FAMERP), São José do Rio Preto 15090-000, São Paulo, Brazil., Goloni-Bertollo EM; Genetics and Molecular Biology Research Unit (UPGEM), Medical School of São José do Rio Preto (FAMERP), São José do Rio Preto 15090-000, São Paulo, Brazil.
المصدر: International journal of molecular sciences [Int J Mol Sci] 2023 Mar 21; Vol. 24 (6). Date of Electronic Publication: 2023 Mar 21.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI, [2000-
مواضيع طبية MeSH: MicroRNAs*/metabolism , Head and Neck Neoplasms*/genetics, Humans ; Cell Line, Tumor ; Epithelial-Mesenchymal Transition/genetics ; Gene Expression Regulation, Neoplastic ; Neoplasm Recurrence, Local/genetics ; Zinc Finger E-box-Binding Homeobox 1/genetics ; Zinc Finger E-box-Binding Homeobox 1/metabolism ; Neoplastic Stem Cells/metabolism ; RNA, Messenger/genetics ; Cell Movement/genetics ; Cell Proliferation
مستخلص: Cancer biologists have focused on studying cancer stem cells (CSCs) because of their ability to self-renew and recapitulate tumor heterogeneity, which increases their resistance to chemotherapy and is associated with cancer relapse. Here, we used two approaches to isolate CSCs: the first involved the metabolic enzyme aldehyde dehydrogenase ALDH, and the second involved the three cell surface markers CD44, CD117, and CD133. ALDH cells showed a higher zinc finger E-box binding homeobox 1 (ZEB1) microRNA (miRNA) expression than CD44/CD117/133 triple-positive cells, which overexpressed miRNA 200c-3p: a well-known microRNA ZEB1 inhibitor. We found that ZEB1 inhibition was driven by miR-101-3p, miR-139-5p, miR-144-3p, miR-199b-5p, and miR-200c-3p and that the FaDu Cell Line inhibition occurred at the mRNA level, whereas HN13 did not affect mRNA expression but decreased protein levels. Furthermore, we demonstrated the ability of the ZEB1 inhibitor miRNAs to modulate CSC-related genes, such as TrkB, ALDH, NANOG, and HIF1A, using transfection technology. We showed that ALDH was upregulated upon ZEB1-suppressed miRNA transfection (Mann-Whitney ** p 101 = 0.009, t -test ** p 139 = 0.009, t -test ** p 144 = 0.002, and t -test *** p 199 = 0.0006). Overall, our study enabled an improved understanding of the role of ZEB1-suppressed miRNAs in CSC biology.
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معلومات مُعتمدة: 2016/20087-1 São Paulo Research Foundation; 310987/2018-0 National Council for Scientific and Technological Development; 001 Coordenação de Aperfeicoamento de Pessoal de Nível Superior
فهرسة مساهمة: Keywords: ZEB1; cancer stem cell; epithelial cell; mesenchymal transition; microRNA targeting
المشرفين على المادة: 0 (MicroRNAs)
0 (Zinc Finger E-box-Binding Homeobox 1)
0 (RNA, Messenger)
0 (ZEB1 protein, human)
0 (MIRN139 microRNA, human)
تواريخ الأحداث: Date Created: 20230329 Date Completed: 20230330 Latest Revision: 20230331
رمز التحديث: 20230331
مُعرف محوري في PubMed: PMC10052136
DOI: 10.3390/ijms24065916
PMID: 36982993
قاعدة البيانات: MEDLINE
الوصف
تدمد:1422-0067
DOI:10.3390/ijms24065916