دورية أكاديمية
Cholesterol depletion affects caveolin-1 expression, migration and invasion of oral tongue squamous cell carcinoma cell lines.
| العنوان: | Cholesterol depletion affects caveolin-1 expression, migration and invasion of oral tongue squamous cell carcinoma cell lines. |
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| المؤلفون: | Nascimento RB; Laboratory of Oral Surgical Pathology, School of Dentistry, Federal University of Bahia, Salvador, Brazil., Risteli M; Cancer and Translational Medicine Research Unit, Faculty of Medicine, University of Oulu, Oulu, Finland; Medical Research Center Oulu, Oulu University Hospital, University of Oulu, Oulu, Finland., Paiva KBS; Laboratory of Extracellular Matrix Biology and Cellular Interaction, Department of Anatomy, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil., Juurikka K; Cancer and Translational Medicine Research Unit, Faculty of Medicine, University of Oulu, Oulu, Finland; Medical Research Center Oulu, Oulu University Hospital, University of Oulu, Oulu, Finland., Rodrigues MFSD; Postgraduate Program in Biophotonics Applied to Health Sciences, Nove de Julho University, UNINOVE, São Paulo, Brazil., Salo TA; Cancer and Translational Medicine Research Unit, Faculty of Medicine, University of Oulu, Oulu, Finland; Department of Oral and Maxillofacial Diseases, University of Helsinki, Helsinki, Finland; HUSLAB, Department of Pathology, Helsinki University Central Hospital, University of Helsinki, Helsinki, Finland., Xavier FCA; Laboratory of Oral Surgical Pathology, School of Dentistry, Federal University of Bahia, Salvador, Brazil. Electronic address: fcalo@ufba.br. |
| المصدر: | Archives of oral biology [Arch Oral Biol] 2023 Jun; Vol. 150, pp. 105675. Date of Electronic Publication: 2023 Mar 16. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Pergamon Press Country of Publication: England NLM ID: 0116711 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-1506 (Electronic) Linking ISSN: 00039969 NLM ISO Abbreviation: Arch Oral Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Oxford : Pergamon Press Original Publication: Oxford, New York [etc.] Pergamon Press. |
| مواضيع طبية MeSH: | Carcinoma, Squamous Cell*/pathology , Tongue Neoplasms*/pathology , Head and Neck Neoplasms* , Myoma*, Humans ; Squamous Cell Carcinoma of Head and Neck ; Caveolin 1/metabolism ; Cadherins/metabolism ; Cell Movement ; Cell Line ; Cholesterol ; Cell Line, Tumor |
| مستخلص: | Introduction: Cholesterol is a key lipid molecule within cell membranes. This is especially true in cavelolas, invaginated membrane nanodomains, which present the protein caveolin-1 (CAV-1). It is important to note that this structure is involved in many cell signalling pathways. Additionally, high cholesterol is seen in different tumor types but little is known in regards to oral tongue squamous cell carcinoma (OTSCC). The aim of this study was to evaluate the influence of cholesterol depletion on primary (SCC-25) and metastatic (HSC-3) OTSCC cell lines. Materials and Methods: Cell membrane fluidity, cell viability, gene and protein expression of CAV-1 and of epithelial-mesenchymal transition (EMT) markers, cell migration in Myogel and invasion-myoma assay were evaluated after cholesterol depletion with methyl-β-cyclodextrin (MβCD - 7.5, 10 or 15 mM) RESULTS: Decreased cell viability and increased membrane fluidity of SCC-25 cells was seen with cholesterol depletion but cell viability was less affected and there was no effect on membrane fluidity in HSC-3. Cholesterol depletion also decreased CAV-1 at 6 h but increased it after 24 h.; both epithelial and mesenchymal EMT genes were upregulated after 6 h, followed by downregulation at 24 h in SCC-25. In HSC-3, CAV-1 was downregulated, and E-cadherin gene (ECAD) was upregulated at 6 h. Only the protein β-catenin in SCC-25 was affected, and cell migration of both cell lines was decreased, affecting SCC-25 more intensely. The invasive capacity within human myoma organotypic model was increased in SCC-25 and decreased in HSC-3. Conclusion: Cholesterol depletion affects CAV-1 and ECAD inversely. This affect also depends on cell type since the invasive capacity was augmented in primary cells while decreased in metastatic cells. Competing Interests: Conflict of interest disclosure The authors declare no conflict of interest. (Copyright © 2023 Elsevier Ltd. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Caveolin-1 (CAV-1); Cholesterol depletion; E-cadherin (ECAD); Epithelial-mesenchymal transition (EMT); Methyl-β-cyclodextrin (MβCD); Tongue squamous cell carcinoma (OTSCC) |
| المشرفين على المادة: | 0 (Caveolin 1) 0 (Cadherins) 97C5T2UQ7J (Cholesterol) |
| تواريخ الأحداث: | Date Created: 20230329 Date Completed: 20230427 Latest Revision: 20230427 |
| رمز التحديث: | 20230427 |
| DOI: | 10.1016/j.archoralbio.2023.105675 |
| PMID: | 36989864 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 1879-1506 |
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| DOI: | 10.1016/j.archoralbio.2023.105675 |