دورية أكاديمية
Multifunctional Exo III-assisted scalability strategy for constructing DNA molecular logic circuits.
| العنوان: | Multifunctional Exo III-assisted scalability strategy for constructing DNA molecular logic circuits. |
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| المؤلفون: | Zeng C; Key Laboratory of Advanced Design and Intelligent Computing, School of Software Engineering, Dalian University, Dalian 116622, China. zccc9802@163.com., Liu X; School of Computer Science and Technology, Dalian University of Technology, Dalian 116024, China. xinliuaisky@mail.dlut.edu.cn., Wang B; Key Laboratory of Advanced Design and Intelligent Computing, School of Software Engineering, Dalian University, Dalian 116622, China. zccc9802@163.com., Qin R; Key Laboratory of Advanced Design and Intelligent Computing, School of Software Engineering, Dalian University, Dalian 116622, China. zccc9802@163.com., Zhang Q; Key Laboratory of Advanced Design and Intelligent Computing, School of Software Engineering, Dalian University, Dalian 116622, China. zccc9802@163.com. |
| المصدر: | The Analyst [Analyst] 2023 May 02; Vol. 148 (9), pp. 1954-1960. Date of Electronic Publication: 2023 May 02. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Royal Society of Chemistry Country of Publication: England NLM ID: 0372652 Publication Model: Electronic Cited Medium: Internet ISSN: 1364-5528 (Electronic) Linking ISSN: 00032654 NLM ISO Abbreviation: Analyst Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Cambridge : Royal Society of Chemistry Original Publication: London : Chemical Society |
| مواضيع طبية MeSH: | DNA* , Exodeoxyribonucleases*, Logic |
| مستخلص: | The construction of logic circuits is critical to DNA computing. Simple and effective scalability methods have been the focus of attention in various fields related to constructing logic circuits. We propose a double-stranded separation (DSS) strategy to facilitate the construction of complex circuits. The strategy combines toehold-mediated strand displacement with exonuclease III (Exo III), which is a multifunctional nuclease. Exo III can quickly recognize an apurinic/apyrimidinic (AP) site. DNA oligos with an AP site can generate an output signal by the strand displacement reaction. However, in contrast to traditional strand displacement reactions, the double-stranded waste from the strand displacement can be further hydrolysed by the endonuclease function of Exo III, thus generating an additional output signal. The DSS strategy allows for the effective scalability of molecular logic circuits, enabling multiple logic computing capabilities simultaneously. In addition, we succeeded in constructing a logic circuit with dual logic functions that provides foundations for more complex circuits in the future and has a broad scope for development in logic computing, biosensing, and nanomachines. |
| المشرفين على المادة: | EC 3.1.11.2 (exodeoxyribonuclease III) 9007-49-2 (DNA) EC 3.1.- (Exodeoxyribonucleases) |
| تواريخ الأحداث: | Date Created: 20230330 Date Completed: 20230503 Latest Revision: 20230503 |
| رمز التحديث: | 20240513 |
| DOI: | 10.1039/d3an00086a |
| PMID: | 36994799 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1364-5528 |
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| DOI: | 10.1039/d3an00086a |