دورية أكاديمية
Interfacing manganese-based carbonaceous nanocomposites with plasma components: insights on protein interaction, structure and opsonization.
| العنوان: | Interfacing manganese-based carbonaceous nanocomposites with plasma components: insights on protein interaction, structure and opsonization. |
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| المؤلفون: | Sanchez Garcia Y; Departamento de Química Fundamental, CCEN, Universidade Federal de Pernambuco, Recife, PE, Brazil., Menezes TM; Departamento de Química Fundamental, CCEN, Universidade Federal de Pernambuco, Recife, PE, Brazil., Rodrigues Barros M; Departamento de Química Fundamental, CCEN, Universidade Federal de Pernambuco, Recife, PE, Brazil., Martins da Silva E; Departamento de Química Fundamental, CCEN, Universidade Federal de Pernambuco, Recife, PE, Brazil., Tavares Ventura G; Instituto de Biofísica, CCS, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil., Frases S; Instituto de Biofísica, CCS, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil., Todeschini AR; Instituto de Biofísica, CCS, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil., Luiz Neves J; Departamento de Química Fundamental, CCEN, Universidade Federal de Pernambuco, Recife, PE, Brazil. |
| المصدر: | Journal of biomolecular structure & dynamics [J Biomol Struct Dyn] 2024 Jan-Feb; Vol. 42 (2), pp. 687-695. Date of Electronic Publication: 2023 Mar 30. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Taylor & Francis Country of Publication: England NLM ID: 8404176 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1538-0254 (Electronic) Linking ISSN: 07391102 NLM ISO Abbreviation: J Biomol Struct Dyn Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: June 2012- : Oxon, UK : Taylor & Francis Original Publication: Guilderland, NY : Adenine Press, [c1983- |
| مواضيع طبية MeSH: | Manganese* , Nanocomposites*/chemistry, Opsonization ; Fluorescence ; Blood Proteins ; Serum Albumin, Bovine/chemistry ; Protein Binding ; Spectrometry, Fluorescence |
| مستخلص: | Metal encapsulation delivers a straightforward strategy to improve miscellaneous nanoparticle properties and qualifies the resulting nanocomposite for exceptional application, including bioimaging, drug release, and theranostic development. Besides crucial applications, investigations associated with the nanocomposite impact on the biological media are highly relevant from a pharmacological viewpoint. Such studies can be conducted by exploring nanocomposite attributes and all aspects of their interaction with proteins existing in biofluids. Based on these aspects, the present work examines manganese-encapsulated carbonaceous nanocomposite (MnCQD) and their interaction with plasma proteins. On one side, the obtained nanocomposite has almost spherical shapes ( ≈ 12 nm in size), an appropriate composition and interesting optical properties for bioimaging applications. On another side, MnCQD quenches the fluorescence of two plasma proteins (BSA and HTF) following a static mechanism, confirming the formation of the MnCQD-BSA and MnCQD-HTF complexes. Although hydrophobic forces guide the stability of both formed complexes, MnCQD binds preferentially to BSA compared to HTF, with affinity constants differing by almost an order of magnitude. Furthermore, HTF and BSA underwent modifications in their secondary structure provoked due to contact with the nanocomposite, which also presented neglectable opsonization levels when exposed to appropriate biological media. These results highlight the MnCQD outstanding potential to be employed in diverse bioapplications.Communicated by Ramaswamy H. Sarma. |
| فهرسة مساهمة: | Keywords: CQD; Carbon dots; albumin; manganese; opsonization; tranferrin |
| المشرفين على المادة: | 42Z2K6ZL8P (Manganese) 0 (Blood Proteins) 27432CM55Q (Serum Albumin, Bovine) |
| تواريخ الأحداث: | Date Created: 20230330 Date Completed: 20240130 Latest Revision: 20240130 |
| رمز التحديث: | 20240130 |
| DOI: | 10.1080/07391102.2023.2195943 |
| PMID: | 36995305 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 1538-0254 |
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| DOI: | 10.1080/07391102.2023.2195943 |