دورية أكاديمية
أعرض في EDS

Molecular confirmation of HIV-1 and HIV-2 coinfections among initially serologically dually-reactive samples from patients living in West Africa.

التفاصيل البيبلوغرافية
العنوان: Molecular confirmation of HIV-1 and HIV-2 coinfections among initially serologically dually-reactive samples from patients living in West Africa.
المؤلفون: Tchounga BK; Programme PACCI, Site ANRS, Abidjan, Côte d'Ivoire.; Elizabeth Glaser Pediatric AIDS Foundation, Yaoundé, Cameroun., Bertine M; Service de Virologie, Université Paris Cité, INSERM, IAME, UMR 1137, AP-HP, Hôpital Bichat-Claude Bernard, Paris, France., Damond F; Service de Virologie, Université Paris Cité, INSERM, IAME, UMR 1137, AP-HP, Hôpital Bichat-Claude Bernard, Paris, France., Ferré VM; Service de Virologie, Université Paris Cité, INSERM, IAME, UMR 1137, AP-HP, Hôpital Bichat-Claude Bernard, Paris, France., Inwoley A; Félix Houphouet-Boigny University, Abidjan, Côte d'Ivoire.; Research and Diagnosis Center for AIDS and Other Infectious Diseases (CeDReS), CHU (University Hospital) of Treichville, Abidjan, Côte d'Ivoire., Boni SP; Programme PACCI, Site ANRS, Abidjan, Côte d'Ivoire., Moisan A; Normandie Univ., UNIROUEN, EA2656, GRAM, CHU de Rouen, Laboratoire de Virologie associé au CNR du VIH, Rouen, France., Plantier JC; Normandie Univ., UNIROUEN, EA2656, GRAM, CHU de Rouen, Laboratoire de Virologie associé au CNR du VIH, Rouen, France., Descamps D; Service de Virologie, Université Paris Cité, INSERM, IAME, UMR 1137, AP-HP, Hôpital Bichat-Claude Bernard, Paris, France., Ekouevi DK; Programme PACCI, Site ANRS, Abidjan, Côte d'Ivoire.; ISPED, Université de Bordeaux & Centre INSERM U1219-Bordeaux Population Health, Bordeaux, France.; Université de Lomé, Faculté des Sciences de la Santé, Département de Santé Publique, Lomé, Togo.; Centre Africain de Recherche en Epidémiologie et en Santé Publique (CARESP), Lomé, Togo., Charpentier C; Service de Virologie, Université Paris Cité, INSERM, IAME, UMR 1137, AP-HP, Hôpital Bichat-Claude Bernard, Paris, France.
المصدر: PloS one [PLoS One] 2023 Mar 31; Vol. 18 (3), pp. e0283602. Date of Electronic Publication: 2023 Mar 31 (Print Publication: 2023).
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE
أسماء مطبوعة: Original Publication: San Francisco, CA : Public Library of Science
مواضيع طبية MeSH: Coinfection*/diagnosis , HIV Infections*/complications , HIV Infections*/diagnosis , HIV Seropositivity* , HIV-1*/genetics, Humans ; Cross-Sectional Studies ; HIV Antibodies ; Cote d'Ivoire/epidemiology ; HIV-2/genetics
مستخلص: Objectives: This study aimed to confirm the co-infection with HIV-1 and HIV-2, among West African patients using in-house HIV type/group enzyme-immuno assays and molecular diagnosis.
Design: A cross-sectional survey was conducted from April 2016 to October 2017 in the biggest HIV clinics of Côte d'Ivoire and Burkina Faso.
Method: A first serological confirmation was done in the referral laboratory using an in-house, indirect immuno-enzymatic essay allowing the qualitative detection of both HIV-1 and HIV-2 antibodies. In order to separately detect anti-HIV-1 and anti-HIV-2 antibodies, a type/group specific enzyme-immuno assay (HIV-GSEIA) was used. To confirm the co-infections, HIV-1 and HIV-2 DNA-qualitative PCR assays were performed.
Results: A total of 91 patients were enrolled in the study and provided blood sample for HIV type confirmatory testing including 13 (14.3%) HIV-2 mono-reactive and 78 (85.7%) HIV-1/HIV-2 dually-reactive based on the HIV testing National Algorithms. The first serological ELISA confirmatory test performed showed that 80 (78.9%) of the 91 participants were dually-reactive. The HIV-GSEIA performed on these 80 serum samples retrieve one 61 HIV-1/HIV-2 dually-reactive samples. HIV-1 and HIV-2 DNA PCR were performed on 54 of the 61 HIV-1/HIV-2 dually-reactive samples and 46 out of 61 (75.4%) samples were found HIV-1/HIV-2 coinfected.
Conclusion: The contribution of type/group specific enzyme-immuno assay to accurately identify HIV-1/HIV-2 coinfections remain suboptimal, emphasizing the need for molecular diagnosis platforms in West Africa, to avail HIV DNA PCR test for the confirmation of HIV-1/HIV-2 co-infections.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: © 2023 Tchounga et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
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المشرفين على المادة: 0 (HIV Antibodies)
تواريخ الأحداث: Date Created: 20230331 Date Completed: 20230404 Latest Revision: 20230419
رمز التحديث: 20230419
مُعرف محوري في PubMed: PMC10065290
DOI: 10.1371/journal.pone.0283602
PMID: 37000823
قاعدة البيانات: MEDLINE
الوصف
تدمد:1932-6203
DOI:10.1371/journal.pone.0283602