دورية أكاديمية
أعرض في EDS

Genome-wide tiled detection of circulating Mycobacterium tuberculosis cell-free DNA using Cas13.

التفاصيل البيبلوغرافية
العنوان: Genome-wide tiled detection of circulating Mycobacterium tuberculosis cell-free DNA using Cas13.
المؤلفون: Thakku SG; Broad Institute of MIT and Harvard, Cambridge, MA, USA., Lirette J; Broad Institute of MIT and Harvard, Cambridge, MA, USA., Murugesan K; Department of Pathology, Stanford University School of Medicine, Stanford, CA, USA., Chen J; Broad Institute of MIT and Harvard, Cambridge, MA, USA., Theron G; DSI-NRF Centre of Excellence for Biomedical Tuberculosis Research and SAMRC Centre for Tuberculosis Research, Division of Molecular Biology and Human Genetics, Faculty of Medicine and Health Sciences, Stellenbosch University, Cape Town, South Africa., Banaei N; Department of Pathology, Stanford University School of Medicine, Stanford, CA, USA.; Department of Medicine, Division of Infectious Diseases and Geographic Medicine, Stanford University School of Medicine, Stanford, CA, USA.; Clinical Microbiology Laboratory, Stanford Health Care, Palo Alto, CA, USA., Blainey PC; Broad Institute of MIT and Harvard, Cambridge, MA, USA.; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.; Koch Institute for Integrative Cancer Research at MIT, Cambridge, MA, USA., Gomez J; Broad Institute of MIT and Harvard, Cambridge, MA, USA., Wong SY; Broad Institute of MIT and Harvard, Cambridge, MA, USA., Hung DT; Broad Institute of MIT and Harvard, Cambridge, MA, USA. dhung@broadinstitute.org.; Department of Genetics, Harvard Medical School, Boston, MA, USA. dhung@broadinstitute.org.; Department of Molecular Biology and Center for Computational and Integrative Biology, Massachusetts General Hospital, Boston, MA, USA. dhung@broadinstitute.org.
المصدر: Nature communications [Nat Commun] 2023 Mar 31; Vol. 14 (1), pp. 1803. Date of Electronic Publication: 2023 Mar 31.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Nature Pub. Group Country of Publication: England NLM ID: 101528555 Publication Model: Electronic Cited Medium: Internet ISSN: 2041-1723 (Electronic) Linking ISSN: 20411723 NLM ISO Abbreviation: Nat Commun Subsets: MEDLINE
أسماء مطبوعة: Original Publication: [London] : Nature Pub. Group
مواضيع طبية MeSH: Mycobacterium tuberculosis*/genetics, Cell-Free System ; DNA, Bacterial/analysis ; DNA, Bacterial/genetics ; Genome, Bacterial ; CRISPR-Cas Systems ; Humans ; Tuberculosis/diagnosis ; Tuberculosis/microbiology
مستخلص: Detection of microbial cell-free DNA (cfDNA) circulating in the bloodstream has emerged as a promising new approach for diagnosing infection. Microbial diagnostics based on cfDNA require assays that can detect rare and highly fragmented pathogen nucleic acids. We now report WATSON (Whole-genome Assay using Tiled Surveillance Of Nucleic acids), a method to detect low amounts of pathogen cfDNA that couples pooled amplification of genomic targets tiled across the genome with pooled CRISPR/Cas13-based detection of these targets. We demonstrate that this strategy of tiling improves cfDNA detection compared to amplification and detection of a single targeted locus. WATSON can detect cfDNA from Mycobacterium tuberculosis in plasma of patients with active pulmonary tuberculosis, a disease that urgently needs accurate, minimally-invasive, field-deployable diagnostics. We thus demonstrate the potential for translating WATSON to a lateral flow platform. WATSON demonstrates the ability to capitalize on the strengths of targeting microbial cfDNA to address the need for point-of-care diagnostic tests for infectious diseases.
(© 2023. The Author(s).)
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المشرفين على المادة: 0 (DNA, Bacterial)
تواريخ الأحداث: Date Created: 20230331 Date Completed: 20230404 Latest Revision: 20230419
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC10064635
DOI: 10.1038/s41467-023-37183-8
PMID: 37002219
قاعدة البيانات: MEDLINE
الوصف
تدمد:2041-1723
DOI:10.1038/s41467-023-37183-8