دورية أكاديمية
Assessment of Tuberculosis Biomarkers in Paratuberculosis-infected Cattle.
العنوان: | Assessment of Tuberculosis Biomarkers in Paratuberculosis-infected Cattle. |
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المؤلفون: | Klepp LI; Institute of Agrobiotechnology and Molecular Biology (IABIMO), INTA-CONICET Hurlingham, Argentina.; Institute of Biotechnology, National Institute of Agricultural Technology, 1686 Hurlingham, Argentina., Colombatti MA; Institute of Agrobiotechnology and Molecular Biology (IABIMO), INTA-CONICET Hurlingham, Argentina.; Institute of Biotechnology, National Institute of Agricultural Technology, 1686 Hurlingham, Argentina., Moyano RD; Institute of Agrobiotechnology and Molecular Biology (IABIMO), INTA-CONICET Hurlingham, Argentina.; Institute of Biotechnology, National Institute of Agricultural Technology, 1686 Hurlingham, Argentina., Romano MI; Institute of Agrobiotechnology and Molecular Biology (IABIMO), INTA-CONICET Hurlingham, Argentina.; Institute of Biotechnology, National Institute of Agricultural Technology, 1686 Hurlingham, Argentina., Malovrh T; Institute for Microbiology and Parasitology, Veterinary Faculty, University of Ljubljana, SI-1000 Ljubljana, Slovenia., Ocepek M; Institute for Microbiology and Parasitology, Veterinary Faculty, University of Ljubljana, SI-1000 Ljubljana, Slovenia., Blanco FC; Institute of Agrobiotechnology and Molecular Biology (IABIMO), INTA-CONICET Hurlingham, Argentina.; Institute of Biotechnology, National Institute of Agricultural Technology, 1686 Hurlingham, Argentina., Bigi F; Institute of Agrobiotechnology and Molecular Biology (IABIMO), INTA-CONICET Hurlingham, Argentina.; Institute of Biotechnology, National Institute of Agricultural Technology, 1686 Hurlingham, Argentina. |
المصدر: | Journal of veterinary research [J Vet Res] 2023 Mar 09; Vol. 67 (1), pp. 55-60. Date of Electronic Publication: 2023 Mar 09 (Print Publication: 2023). |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Sciendo Country of Publication: Poland NLM ID: 101696630 Publication Model: eCollection Cited Medium: Print ISSN: 2450-7393 (Print) Linking ISSN: 24507393 NLM ISO Abbreviation: J Vet Res Subsets: PubMed not MEDLINE |
أسماء مطبوعة: | Publication: [Warsaw, Poland] : Sciendo Original Publication: Pulawy : National Veterinary Research Institute, 2016- |
مستخلص: | Introduction: Mycobacterium bovis and Mycobacterium avium subsp. paratuberculosis , respectively the causative agents of bovine tuberculosis (bTB) and bovine paratuberculosis (PTB), share a high number of antigenic proteins. This characteristics makes the differential diagnosis of the diseases difficult. The interferon gamma (IFN-γ), C-X-C motif chemokine ligand 10 (CXCL10), matrix metallopeptidase 9 (MMP9), interleukin 22 (IL-22) and thrombospondin 1 (THBS1) bovine genes have already been shown to be accurate transcriptional biomarkers of bTB. In order to improve the diagnosis of bTB and PTB, in the present study we evaluated the risk of false positivity of these bTB biomarkers in cattle with PTB. Material and Methods: The transcription of these genes was studied in 13 PTB-infected cattle, using Mycobacterium avium subsp. paratuberculosis (MAP)-stimulated peripheral blood mononuclear cells (PBMC). Results: Overall, the levels of IFN-γ, CXCL10, MMP9 and IL-22 transcripts in MAP-stimulated PBMC failed to differentiate animals with PTB from healthy animals. However, as bTB-afflicted cattle do, the MAP-infected group also displayed a lower level of THBS1 transcription than the non-infected animals. Conclusion: The results of this study add new specificity attributes to the levels of transcription of IFN-γ, CXCL10, MMP9 and IL-22 as biomarkers for bTB. Competing Interests: Conflict of Interest Conflict of Interests Statement: The authors declare that there is no conflict of interests regarding the publication of this article. (© 2023 L.I. Klepp et al., published by Sciendo.) |
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فهرسة مساهمة: | Keywords: biomarkers; bovine paratuberculosis; bovine tuberculosis |
تواريخ الأحداث: | Date Created: 20230403 Latest Revision: 20230404 |
رمز التحديث: | 20230404 |
مُعرف محوري في PubMed: | PMC10062049 |
DOI: | 10.2478/jvetres-2023-0007 |
PMID: | 37008763 |
قاعدة البيانات: | MEDLINE |
تدمد: | 2450-7393 |
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DOI: | 10.2478/jvetres-2023-0007 |