دورية أكاديمية
أعرض في EDS

Assessment of Tuberculosis Biomarkers in Paratuberculosis-infected Cattle.

التفاصيل البيبلوغرافية
العنوان: Assessment of Tuberculosis Biomarkers in Paratuberculosis-infected Cattle.
المؤلفون: Klepp LI; Institute of Agrobiotechnology and Molecular Biology (IABIMO), INTA-CONICET Hurlingham, Argentina.; Institute of Biotechnology, National Institute of Agricultural Technology, 1686 Hurlingham, Argentina., Colombatti MA; Institute of Agrobiotechnology and Molecular Biology (IABIMO), INTA-CONICET Hurlingham, Argentina.; Institute of Biotechnology, National Institute of Agricultural Technology, 1686 Hurlingham, Argentina., Moyano RD; Institute of Agrobiotechnology and Molecular Biology (IABIMO), INTA-CONICET Hurlingham, Argentina.; Institute of Biotechnology, National Institute of Agricultural Technology, 1686 Hurlingham, Argentina., Romano MI; Institute of Agrobiotechnology and Molecular Biology (IABIMO), INTA-CONICET Hurlingham, Argentina.; Institute of Biotechnology, National Institute of Agricultural Technology, 1686 Hurlingham, Argentina., Malovrh T; Institute for Microbiology and Parasitology, Veterinary Faculty, University of Ljubljana, SI-1000 Ljubljana, Slovenia., Ocepek M; Institute for Microbiology and Parasitology, Veterinary Faculty, University of Ljubljana, SI-1000 Ljubljana, Slovenia., Blanco FC; Institute of Agrobiotechnology and Molecular Biology (IABIMO), INTA-CONICET Hurlingham, Argentina.; Institute of Biotechnology, National Institute of Agricultural Technology, 1686 Hurlingham, Argentina., Bigi F; Institute of Agrobiotechnology and Molecular Biology (IABIMO), INTA-CONICET Hurlingham, Argentina.; Institute of Biotechnology, National Institute of Agricultural Technology, 1686 Hurlingham, Argentina.
المصدر: Journal of veterinary research [J Vet Res] 2023 Mar 09; Vol. 67 (1), pp. 55-60. Date of Electronic Publication: 2023 Mar 09 (Print Publication: 2023).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Sciendo Country of Publication: Poland NLM ID: 101696630 Publication Model: eCollection Cited Medium: Print ISSN: 2450-7393 (Print) Linking ISSN: 24507393 NLM ISO Abbreviation: J Vet Res Subsets: PubMed not MEDLINE
أسماء مطبوعة: Publication: [Warsaw, Poland] : Sciendo
Original Publication: Pulawy : National Veterinary Research Institute, 2016-
مستخلص: Introduction: Mycobacterium bovis and Mycobacterium avium subsp. paratuberculosis , respectively the causative agents of bovine tuberculosis (bTB) and bovine paratuberculosis (PTB), share a high number of antigenic proteins. This characteristics makes the differential diagnosis of the diseases difficult. The interferon gamma (IFN-γ), C-X-C motif chemokine ligand 10 (CXCL10), matrix metallopeptidase 9 (MMP9), interleukin 22 (IL-22) and thrombospondin 1 (THBS1) bovine genes have already been shown to be accurate transcriptional biomarkers of bTB. In order to improve the diagnosis of bTB and PTB, in the present study we evaluated the risk of false positivity of these bTB biomarkers in cattle with PTB.
Material and Methods: The transcription of these genes was studied in 13 PTB-infected cattle, using Mycobacterium avium subsp. paratuberculosis (MAP)-stimulated peripheral blood mononuclear cells (PBMC).
Results: Overall, the levels of IFN-γ, CXCL10, MMP9 and IL-22 transcripts in MAP-stimulated PBMC failed to differentiate animals with PTB from healthy animals. However, as bTB-afflicted cattle do, the MAP-infected group also displayed a lower level of THBS1 transcription than the non-infected animals.
Conclusion: The results of this study add new specificity attributes to the levels of transcription of IFN-γ, CXCL10, MMP9 and IL-22 as biomarkers for bTB.
Competing Interests: Conflict of Interest Conflict of Interests Statement: The authors declare that there is no conflict of interests regarding the publication of this article.
(© 2023 L.I. Klepp et al., published by Sciendo.)
References: Vet Pathol. 2013 May;50(3):488-99. (PMID: 23169912)
Autophagy. 2022 Feb;18(2):391-408. (PMID: 34074211)
Sci Rep. 2019 Oct 16;9(1):14845. (PMID: 31619718)
Front Genet. 2021 Oct 15;12:735147. (PMID: 34721525)
Vet Res. 2015 Jun 19;46:62. (PMID: 26092254)
Vet Med Int. 2021 Dec 29;2021:7336848. (PMID: 35003619)
Front Immunol. 2014 Nov 05;5:536. (PMID: 25414700)
Vet Med Int. 2012;2012:236205. (PMID: 22737588)
PLoS One. 2012;7(7):e41066. (PMID: 22815916)
J Vet Med B Infect Dis Vet Public Health. 2002 Mar;49(2):89-96. (PMID: 12002425)
Infect Immun. 2002 Oct;70(10):5494-502. (PMID: 12228275)
Nucleic Acids Res. 2002 May 1;30(9):e36. (PMID: 11972351)
J Agromedicine. 2021 Jul;26(3):334-339. (PMID: 32478614)
BMC Microbiol. 2006 Oct 04;6:87. (PMID: 17020599)
Aust Vet J. 1990 Apr;67(4):134-7. (PMID: 2115767)
PLoS One. 2018 Apr 26;13(4):e0196502. (PMID: 29698503)
Vet Res. 2014 Oct 02;45:96. (PMID: 25294045)
Vet Res. 2006 Jul-Aug;37(4):593-606. (PMID: 16701065)
Vet Microbiol. 2012 Apr 23;156(1-2):183-8. (PMID: 22138619)
Res Vet Sci. 2019 Feb;122:7-14. (PMID: 30447501)
Vaccine. 2011 Jun 24;29(29-30):4709-19. (PMID: 21565243)
Vet Immunol Immunopathol. 2011 Jun 15;141(3-4):258-66. (PMID: 21477870)
PLoS One. 2012;7(4):e34590. (PMID: 22509329)
فهرسة مساهمة: Keywords: biomarkers; bovine paratuberculosis; bovine tuberculosis
تواريخ الأحداث: Date Created: 20230403 Latest Revision: 20230404
رمز التحديث: 20230404
مُعرف محوري في PubMed: PMC10062049
DOI: 10.2478/jvetres-2023-0007
PMID: 37008763
قاعدة البيانات: MEDLINE
الوصف
تدمد:2450-7393
DOI:10.2478/jvetres-2023-0007