دورية أكاديمية
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Improving acetoin production through construction of a genome-scale metabolic model.

التفاصيل البيبلوغرافية
العنوان: Improving acetoin production through construction of a genome-scale metabolic model.
المؤلفون: Qian J; School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, Nanjing, 210023, PR China., Wang Y; School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, Nanjing, 210023, PR China., Liu X; School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, Nanjing, 210023, PR China., Hu Z; School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, Nanjing, 210023, PR China., Xu N; College of Bioscience and Biotechnology, Yangzhou University, Yangzhou, 225009, PR China., Wang Y; School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, Nanjing, 210023, PR China. Electronic address: wangyt@nnu.edu.cn., Shi T; School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, Nanjing, 210023, PR China. Electronic address: tqshi@njnu.edu.cn., Ye C; School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, Nanjing, 210023, PR China. Electronic address: chaoye09@njnu.edu.cn.
المصدر: Computers in biology and medicine [Comput Biol Med] 2023 May; Vol. 158, pp. 106833. Date of Electronic Publication: 2023 Mar 31.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: United States NLM ID: 1250250 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0534 (Electronic) Linking ISSN: 00104825 NLM ISO Abbreviation: Comput Biol Med Subsets: MEDLINE
أسماء مطبوعة: Publication: New York : Elsevier
Original Publication: New York, Pergamon Press.
مواضيع طبية MeSH: Acetoin*/metabolism , Metabolic Engineering*/methods, Fermentation ; Metabolic Networks and Pathways/genetics
مستخلص: Acetoin was widely used in food, medicine, and other industries, because of its unique fragrance. Bacillus amyloliquefaciens was recognized as a safe strain and a promising acetoin producer in fermentation. However, due to the complexity of its metabolic network, it had not been fully utilized. Therefore, a genome-scale metabolic network model (iJYQ746) of B. amyloliquefaciens was constructed in this study, containing 746 genes, 1736 reactions, and 1611 metabolites. The results showed that Mg 2+ , Mn 2+ , and Fe 2+ have inhibitory effects on acetoin. When the stirring speed was 400 rpm, the maximum titer was 49.8 g L -1 . Minimization of metabolic adjustments (MOMA) was used to identify potential metabolic modification targets 2-oxoglutarate aminotransferase (serC, EC 2.6.1.52) and glucose-6-phosphate isomerase (pgi, EC 5.3.1.9). These targets could effectively accumulate acetoin by increasing pyruvate content, and the acetoin synthesis rate was increased by 610% and 10%, respectively. This provides a theoretical basis for metabolic engineering to reasonably transform B. amyloliquefaciens and produce acetoin.
Competing Interests: Declaration of competing interest The authors declare no competing interests.
(Copyright © 2023 Elsevier Ltd. All rights reserved.)
فهرسة مساهمة: Keywords: Acetoin; Bacillus amyloliquefaciens; Genome-scale metabolic network model; Potential targets
المشرفين على المادة: BG4D34CO2H (Acetoin)
تواريخ الأحداث: Date Created: 20230404 Date Completed: 20230421 Latest Revision: 20230421
رمز التحديث: 20230421
DOI: 10.1016/j.compbiomed.2023.106833
PMID: 37015178
قاعدة البيانات: MEDLINE
الوصف
تدمد:1879-0534
DOI:10.1016/j.compbiomed.2023.106833