دورية أكاديمية
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A lysosome membrane regeneration pathway depends on TBC1D15 and autophagic lysosomal reformation proteins.

التفاصيل البيبلوغرافية
العنوان: A lysosome membrane regeneration pathway depends on TBC1D15 and autophagic lysosomal reformation proteins.
المؤلفون: Bhattacharya A; Institute of Biochemistry II, Faculty of Medicine, Goethe University, Frankfurt, Germany.; Buchmann Institute for Molecular Life Sciences, Goethe University, Frankfurt, Germany., Mukherjee R; Institute of Biochemistry II, Faculty of Medicine, Goethe University, Frankfurt, Germany.; Buchmann Institute for Molecular Life Sciences, Goethe University, Frankfurt, Germany.; Max Planck Institute of Biophysics, Frankfurt, Germany., Kuncha SK; Institute of Biochemistry II, Faculty of Medicine, Goethe University, Frankfurt, Germany.; Buchmann Institute for Molecular Life Sciences, Goethe University, Frankfurt, Germany., Brunstein ME; Institute of Biochemistry II, Faculty of Medicine, Goethe University, Frankfurt, Germany., Rathore R; Institute of Biochemistry II, Faculty of Medicine, Goethe University, Frankfurt, Germany., Junek S; Max Planck Institute of Biophysics, Frankfurt, Germany.; Max Planck Institute for Brain Research, Frankfurt, Germany., Münch C; Institute of Biochemistry II, Faculty of Medicine, Goethe University, Frankfurt, Germany., Dikic I; Institute of Biochemistry II, Faculty of Medicine, Goethe University, Frankfurt, Germany. dikic@biochem2.uni-frankfurt.de.; Buchmann Institute for Molecular Life Sciences, Goethe University, Frankfurt, Germany. dikic@biochem2.uni-frankfurt.de.; Max Planck Institute of Biophysics, Frankfurt, Germany. dikic@biochem2.uni-frankfurt.de.; Fraunhofer Institute for Translational Medicine and Pharmacology (ITMP), Frankfurt, Germany. dikic@biochem2.uni-frankfurt.de.
المصدر: Nature cell biology [Nat Cell Biol] 2023 May; Vol. 25 (5), pp. 685-698. Date of Electronic Publication: 2023 Apr 06.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Macmillan Magazines Ltd Country of Publication: England NLM ID: 100890575 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1476-4679 (Electronic) Linking ISSN: 14657392 NLM ISO Abbreviation: Nat Cell Biol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : Macmillan Magazines Ltd., [1999-
مواضيع طبية MeSH: Dynamin II*/metabolism , Autophagy*, Intracellular Membranes/metabolism ; GTPase-Activating Proteins/genetics ; GTPase-Activating Proteins/metabolism ; Lysosomes/metabolism
مستخلص: Acute lysosomal membrane damage reduces the cellular population of functional lysosomes. However, these damaged lysosomes have a remarkable recovery potential independent of lysosomal biogenesis and remain unaffected in cells depleted in TFEB and TFE3. We combined proximity-labelling-based proteomics, biochemistry and high-resolution microscopy to unravel a lysosomal membrane regeneration pathway that depends on ATG8, the lysosomal membrane protein LIMP2, the RAB7 GTPase-activating protein TBC1D15 and proteins required for autophagic lysosomal reformation, including dynamin-2, kinesin-5B and clathrin. Following lysosomal damage, LIMP2 acts as a lysophagy receptor to bind ATG8, which in turn recruits TBC1D15 to damaged membranes. TBC1D15 interacts with ATG8 proteins on damaged lysosomes and provides a scaffold to assemble and stabilize the autophagic lysosomal reformation machinery. This potentiates the formation of lysosomal tubules and subsequent dynamin-2-dependent scission. TBC1D15-mediated lysosome regeneration was also observed in a cell culture model of oxalate nephropathy.
(© 2023. The Author(s), under exclusive licence to Springer Nature Limited.)
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المشرفين على المادة: EC 3.6.5.5 (Dynamin II)
0 (GTPase-Activating Proteins)
تواريخ الأحداث: Date Created: 20230406 Date Completed: 20230517 Latest Revision: 20230629
رمز التحديث: 20231215
DOI: 10.1038/s41556-023-01125-9
PMID: 37024685
قاعدة البيانات: MEDLINE
الوصف
تدمد:1476-4679
DOI:10.1038/s41556-023-01125-9