دورية أكاديمية
A lysosome membrane regeneration pathway depends on TBC1D15 and autophagic lysosomal reformation proteins.
العنوان: | A lysosome membrane regeneration pathway depends on TBC1D15 and autophagic lysosomal reformation proteins. |
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المؤلفون: | Bhattacharya A; Institute of Biochemistry II, Faculty of Medicine, Goethe University, Frankfurt, Germany.; Buchmann Institute for Molecular Life Sciences, Goethe University, Frankfurt, Germany., Mukherjee R; Institute of Biochemistry II, Faculty of Medicine, Goethe University, Frankfurt, Germany.; Buchmann Institute for Molecular Life Sciences, Goethe University, Frankfurt, Germany.; Max Planck Institute of Biophysics, Frankfurt, Germany., Kuncha SK; Institute of Biochemistry II, Faculty of Medicine, Goethe University, Frankfurt, Germany.; Buchmann Institute for Molecular Life Sciences, Goethe University, Frankfurt, Germany., Brunstein ME; Institute of Biochemistry II, Faculty of Medicine, Goethe University, Frankfurt, Germany., Rathore R; Institute of Biochemistry II, Faculty of Medicine, Goethe University, Frankfurt, Germany., Junek S; Max Planck Institute of Biophysics, Frankfurt, Germany.; Max Planck Institute for Brain Research, Frankfurt, Germany., Münch C; Institute of Biochemistry II, Faculty of Medicine, Goethe University, Frankfurt, Germany., Dikic I; Institute of Biochemistry II, Faculty of Medicine, Goethe University, Frankfurt, Germany. dikic@biochem2.uni-frankfurt.de.; Buchmann Institute for Molecular Life Sciences, Goethe University, Frankfurt, Germany. dikic@biochem2.uni-frankfurt.de.; Max Planck Institute of Biophysics, Frankfurt, Germany. dikic@biochem2.uni-frankfurt.de.; Fraunhofer Institute for Translational Medicine and Pharmacology (ITMP), Frankfurt, Germany. dikic@biochem2.uni-frankfurt.de. |
المصدر: | Nature cell biology [Nat Cell Biol] 2023 May; Vol. 25 (5), pp. 685-698. Date of Electronic Publication: 2023 Apr 06. |
نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
اللغة: | English |
بيانات الدورية: | Publisher: Macmillan Magazines Ltd Country of Publication: England NLM ID: 100890575 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1476-4679 (Electronic) Linking ISSN: 14657392 NLM ISO Abbreviation: Nat Cell Biol Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: London : Macmillan Magazines Ltd., [1999- |
مواضيع طبية MeSH: | Dynamin II*/metabolism , Autophagy*, Intracellular Membranes/metabolism ; GTPase-Activating Proteins/genetics ; GTPase-Activating Proteins/metabolism ; Lysosomes/metabolism |
مستخلص: | Acute lysosomal membrane damage reduces the cellular population of functional lysosomes. However, these damaged lysosomes have a remarkable recovery potential independent of lysosomal biogenesis and remain unaffected in cells depleted in TFEB and TFE3. We combined proximity-labelling-based proteomics, biochemistry and high-resolution microscopy to unravel a lysosomal membrane regeneration pathway that depends on ATG8, the lysosomal membrane protein LIMP2, the RAB7 GTPase-activating protein TBC1D15 and proteins required for autophagic lysosomal reformation, including dynamin-2, kinesin-5B and clathrin. Following lysosomal damage, LIMP2 acts as a lysophagy receptor to bind ATG8, which in turn recruits TBC1D15 to damaged membranes. TBC1D15 interacts with ATG8 proteins on damaged lysosomes and provides a scaffold to assemble and stabilize the autophagic lysosomal reformation machinery. This potentiates the formation of lysosomal tubules and subsequent dynamin-2-dependent scission. TBC1D15-mediated lysosome regeneration was also observed in a cell culture model of oxalate nephropathy. (© 2023. The Author(s), under exclusive licence to Springer Nature Limited.) |
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المشرفين على المادة: | EC 3.6.5.5 (Dynamin II) 0 (GTPase-Activating Proteins) |
تواريخ الأحداث: | Date Created: 20230406 Date Completed: 20230517 Latest Revision: 20230629 |
رمز التحديث: | 20231215 |
DOI: | 10.1038/s41556-023-01125-9 |
PMID: | 37024685 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1476-4679 |
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DOI: | 10.1038/s41556-023-01125-9 |