دورية أكاديمية
أعرض في EDS

pH-Selective Reactions to Selectively Reduce Cancer Cell Proliferation: Effect of CaS Nanostructures in Human Skin Melanoma and Benign Fibroblasts.

التفاصيل البيبلوغرافية
العنوان: pH-Selective Reactions to Selectively Reduce Cancer Cell Proliferation: Effect of CaS Nanostructures in Human Skin Melanoma and Benign Fibroblasts.
المؤلفون: Martínez OMR; Department of Biology, School of Arts and Sciences, University of Puerto Rico at Mayaguez, Mayaguez, PR 00682, USA., Ramos MAN; Department of Biology, School of Arts and Sciences, University of Puerto Rico at Mayaguez, Mayaguez, PR 00682, USA., Acevedo AAS; Department of Chemistry, School of Arts and Sciences, University of Puerto Rico at Mayaguez, Mayaguez, PR 00682, USA., Colón CCC; Department of Chemistry, School of Arts and Sciences, University of Puerto Rico at Mayaguez, Mayaguez, PR 00682, USA., Ramos DM; Department of Biology, School of Arts and Sciences, University of Puerto Rico at Mayaguez, Mayaguez, PR 00682, USA., Rivera CC; Department of Biology, School of Arts and Sciences, University of Puerto Rico at Mayaguez, Mayaguez, PR 00682, USA., Rosario MEC; Department of Chemistry, School of Arts and Sciences, University of Puerto Rico at Mayaguez, Mayaguez, PR 00682, USA.
المصدر: BioChem [Biochem (Basel)] 2023 Mar; Vol. 3 (1), pp. 15-30. Date of Electronic Publication: 2023 Jan 18.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 9918434587906676 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2673-6411 (Electronic) Linking ISSN: 26736411 NLM ISO Abbreviation: Biochem (Basel) Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Basel : MDPI, 2021-
مستخلص: An acidic extracellular pH value (pH e ) is characteristic of many cancers, in contrast to the physiologic pH e found in most benign cells. This difference in pH offers a unique opportunity to design and engineer chemicals that can be employed for pH-selective reactions in the extracellular fluid of cancer cells. The viability of human skin melanoma and corresponding fibroblasts exposed to CaS dispersions is reported. The viability of melanoma cells decreases with CaS dispersion concentration and reaches 57% at 3%, a value easily distinguishable from melanoma control experiments. In contrast, the viability of benign fibroblasts remains nearly constant within experimental error over the range of dispersion concentrations studied. The CaS dispersions facilitate vinculin delocalization in the cytoplasmic fluid, a result consistent with improved focal adhesion kinase (FAK) regulation in melanoma cells. Thermodynamic considerations are consistent with the formation of H 2 S from CaS in the presence of protons. The thermodynamic prediction is verified in independent experiments with solid CaS and acidic aqueous solutions. The amount of H 2 S formed decreases with pH. An activation energy for the process of (30 ± 10) kJ/mol in the temperature range of 280 to 330 K is estimated from initial rate measurements as a function of temperature. The total Gibbs energy minimization approach was employed to establish the distribution of sulfides-including H 2 S in the gas and aqueous phases-from the dissociation of CaS as a function of pH to mimic physiologically relevant pH values. Theoretical calculations suggest that partially protonated CaS in solution can be stable until the sulfur atom bonds to two hydrogen atoms, resulting in the formation of Ca 2+ and H 2 S , which can be solvated and/or released to the gas phase. Our results are consistent with a model in which CaS is dissociated in the extracellular fluid of melanoma cells selectively. The results are discussed in the context of the potential biomedical applications of CaS dispersions in cancer therapies.
Competing Interests: Conflicts of Interest: The authors declare no conflict of interest.
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معلومات مُعتمدة: R25 DK098067 United States DK NIDDK NIH HHS; R25 GM127191 United States GM NIGMS NIH HHS
فهرسة مساهمة: Keywords: calcium sulfide nanostructures; extracellular pH; melanoma
تواريخ الأحداث: Date Created: 20230410 Latest Revision: 20240607
رمز التحديث: 20240607
مُعرف محوري في PubMed: PMC10079261
DOI: 10.3390/biochem3010002
PMID: 37035583
قاعدة البيانات: MEDLINE
الوصف
تدمد:2673-6411
DOI:10.3390/biochem3010002