دورية أكاديمية
أعرض في EDS

Genetic and biochemical characterization of BIM-1, a novel acquired subgroup B1 MBL found in a Pseudomonas sp. strain from the Brazilian Amazon region.

التفاصيل البيبلوغرافية
العنوان: Genetic and biochemical characterization of BIM-1, a novel acquired subgroup B1 MBL found in a Pseudomonas sp. strain from the Brazilian Amazon region.
المؤلفون: Souza CO; Seção de Bacteriologia e Micologia, Instituto Evandro Chagas (IEC), Ananindeua, PA, Brazil., Cayô R; Universidade Federal de São Paulo (UNIFESP), Laboratório ALERTA, Disciplina de Infectologia, Departamento de Medicina, Escola Paulista de Medicina (EPM), São Paulo, SP, Brazil.; Universidade Federal de São Paulo (UNIFESP), Laboratório de Bacteriologia e Imunologia (LIB), Setor de Biologia Molecular, Microbiologia e Imunologia, Departamento de Ciências Biológicas (DCB), Instituto de Ciências Ambientais, Químicas e Farmacêuticas (ICAQF), Diadema, SP, Brazil., Lima KVB; Seção de Bacteriologia e Micologia, Instituto Evandro Chagas (IEC), Ananindeua, PA, Brazil., Brasiliense DM; Seção de Bacteriologia e Micologia, Instituto Evandro Chagas (IEC), Ananindeua, PA, Brazil., Streling AP; Universidade Federal de São Paulo (UNIFESP), Laboratório ALERTA, Disciplina de Infectologia, Departamento de Medicina, Escola Paulista de Medicina (EPM), São Paulo, SP, Brazil., Siqueira AV; Universidade Federal de São Paulo (UNIFESP), Laboratório ALERTA, Disciplina de Infectologia, Departamento de Medicina, Escola Paulista de Medicina (EPM), São Paulo, SP, Brazil., Alberto-Lei F; Universidade Federal de São Paulo (UNIFESP), Laboratório ALERTA, Disciplina de Infectologia, Departamento de Medicina, Escola Paulista de Medicina (EPM), São Paulo, SP, Brazil., Leal JT; Universidade Federal de São Paulo (UNIFESP), Laboratório ALERTA, Disciplina de Infectologia, Departamento de Medicina, Escola Paulista de Medicina (EPM), São Paulo, SP, Brazil., Nodari CS; Universidade Federal de São Paulo (UNIFESP), Laboratório ALERTA, Disciplina de Infectologia, Departamento de Medicina, Escola Paulista de Medicina (EPM), São Paulo, SP, Brazil., Pérez-Chaparro PJ; Department of Clinical Analysis, School of Pharmacy (FCF), Universidade de São Paulo (USP), São Paulo, SP, Brazil., Lima LNGC; Seção de Bacteriologia e Micologia, Instituto Evandro Chagas (IEC), Ananindeua, PA, Brazil., Lima MO; Seção de Meio Ambiente, Instituto Evandro Chagas (IEC), Ananindeua, PA, Brazil.; Programa de Pós-Graduação em Epidemiologia e Vigilância em Saúde (PPGEVS), Instituto Evandro Chagas (IEC), Ananindeua, PA, Brazil.; Universidade Federal do Pará (UFPA), Programa de Pós-Graduação em Ecologia Aquática e Pesca (PPGEAP), Belém, PA, Brazil., Costa BNS; Seção de Meio Ambiente, Instituto Evandro Chagas (IEC), Ananindeua, PA, Brazil.; Universidade Federal do Pará (UFPA), Programa de Pós-Graduação em Ecologia Aquática e Pesca (PPGEAP), Belém, PA, Brazil., De Queiroz TKL; Seção de Meio Ambiente, Instituto Evandro Chagas (IEC), Ananindeua, PA, Brazil.; Universidade Federal do Pará (UFPA), Programa de Pós-Graduação em Ecologia Aquática e Pesca (PPGEAP), Belém, PA, Brazil., Silva PJSN; Universidade Federal de São Paulo (UNIFESP), Laboratório de Enzimologia, Department of Biophysics, Escola Paulista de Medicina (EPM), São Paulo, SP, Brazil., Mamizuka EM; Department of Clinical Analysis, School of Pharmacy (FCF), Universidade de São Paulo (USP), São Paulo, SP, Brazil., Marcondes MF; Universidade Federal de São Paulo (UNIFESP), Laboratório de Enzimologia, Department of Biophysics, Escola Paulista de Medicina (EPM), São Paulo, SP, Brazil., Mcculloch JA; Department of Clinical Analysis, School of Pharmacy (FCF), Universidade de São Paulo (USP), São Paulo, SP, Brazil., Gales AC; Universidade Federal de São Paulo (UNIFESP), Laboratório ALERTA, Disciplina de Infectologia, Departamento de Medicina, Escola Paulista de Medicina (EPM), São Paulo, SP, Brazil.
المصدر: The Journal of antimicrobial chemotherapy [J Antimicrob Chemother] 2023 Jun 01; Vol. 78 (6), pp. 1359-1366.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Oxford University Press Country of Publication: England NLM ID: 7513617 Publication Model: Print Cited Medium: Internet ISSN: 1460-2091 (Electronic) Linking ISSN: 03057453 NLM ISO Abbreviation: J Antimicrob Chemother Subsets: MEDLINE
أسماء مطبوعة: Publication: 1997- : London : Oxford University Press
Original Publication: London, New York, Academic Press.
مواضيع طبية MeSH: Pseudomonas*/genetics , beta-Lactamases*/genetics, Brazil/epidemiology ; Pseudomonas aeruginosa/genetics ; Anti-Bacterial Agents/pharmacology ; beta-Lactams ; Microbial Sensitivity Tests
مستخلص: Objectives: To characterize a novel acquired MBL, BIM-1, in a Pseudomonas #2 (subgroup P. guariconensis) strain isolated from the Aurá river located in the Brazilian Amazon hydrographic basin.
Methods: WGS using an Illumina® MiSeq System was used to characterize the genome of Pseudomonas sp. IEC33019 strain. Southern blotting/hybridization assays were performed to confirm the location of the MBL-encoding gene, blaBIM-1 (Belém Imipenemase). Antimicrobial susceptibility testing, cloning, and biochemical and phenotypic characterization were performed to determine BIM-1 kinetics.
Results: The IEC33019 strain showed high resistance rates to β-lactams, ciprofloxacin and aminoglycosides, being susceptible only to polymyxins and susceptible, increased exposure to aztreonam. WGS analysis revealed a novel acquired MBL-encoding gene, blaBIM-1, found as a gene cassette inserted into a class 1 integron (In1326) that also carried qnrVC1 and aadA11e. In1326 was located in a complex transposon, Tn7122, carried by a 52.7 kb conjugative plasmid (pIEC33019) with a toxin/antitoxin system (vapB/vapC). BIM-1 belongs to the molecular subgroup B1 and shares 70.2% and 64.9% similarity with SIM-1 and IMP-1, respectively. Kinetics analysis of BIM-1 showed hydrolytic activity against all β-lactams tested.
Conclusions: BIM-1 is a novel acquired MBL encoded by a gene carried by mobile genetic elements, which can be transferred to other Gram-negative bacilli (GNB). Because the IEC33019 strain was recovered from a river impacted by a populous metropolitan region with poor basic sanitation and served by limited potable freshwater, it would be important to establish the role of the BIM-1-producing GNB as nosocomial pathogens and/or as colonizers of the riverside population in this geographical region.
(© The Author(s) 2023. Published by Oxford University Press on behalf of British Society for Antimicrobial Chemotherapy.)
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المشرفين على المادة: EC 3.5.2.6 (beta-Lactamases)
0 (Anti-Bacterial Agents)
0 (beta-Lactams)
تواريخ الأحداث: Date Created: 20230411 Date Completed: 20230602 Latest Revision: 20230612
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC10232235
DOI: 10.1093/jac/dkad077
PMID: 37038995
قاعدة البيانات: MEDLINE
الوصف
تدمد:1460-2091
DOI:10.1093/jac/dkad077