دورية أكاديمية
The anthelmintic potential of Bacillus thuringiensis to counter the anthelmintic resistance against Haemonchus contortus.
| العنوان: | The anthelmintic potential of Bacillus thuringiensis to counter the anthelmintic resistance against Haemonchus contortus. |
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| المؤلفون: | Panhwer SN; Laboratory of Animal Disease Model, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang Chengdu, Sichuan, China., Gadahi JA; Department of Veterinary Parasitology, Sindh Agriculture University, Tandojam, Pakistan. Electronic address: jagadahi@sau.edu.pk., Luo Q; Laboratory of Animal Disease Model, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang Chengdu, Sichuan, China., Huang C; Laboratory of Animal Disease Model, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang Chengdu, Sichuan, China., Liu W; Laboratory of Animal Disease Model, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang Chengdu, Sichuan, China., Jia L; Laboratory of Animal Disease Model, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang Chengdu, Sichuan, China., Chen Z; Laboratory of Animal Disease Model, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang Chengdu, Sichuan, China. Electronic address: chzhli75@sicau.edu.cn. |
| المصدر: | Experimental parasitology [Exp Parasitol] 2023 Jul; Vol. 250, pp. 108533. Date of Electronic Publication: 2023 Apr 16. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Academic Press Country of Publication: United States NLM ID: 0370713 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1090-2449 (Electronic) Linking ISSN: 00144894 NLM ISO Abbreviation: Exp Parasitol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Orlando, FL : Academic Press Original Publication: New York. |
| مواضيع طبية MeSH: | Haemonchus* , Bacillus thuringiensis* , Anthelmintics*/pharmacology , Anthelmintics*/therapeutic use , Anthelmintics*/metabolism , Haemonchiasis*/drug therapy , Haemonchiasis*/veterinary , Sheep Diseases*/drug therapy, Animals ; Male ; Sheep/genetics ; RNA, Ribosomal, 16S ; Bacterial Proteins/analysis ; Parasite Egg Count/veterinary |
| مستخلص: | Haemonchus contortus (H. contortus) has developed resistance to nearly all available anthelmintic medications. Hence, alternative strategies are required to counter anthelmintic resistance. The present study investigated the anthelmintic potential of Bacillus thuringiensis (B. thuringiensis) against H. contortus. Bacterial spp were identified by conventional methods and confirmed by PCR; In addition, PCR amplification of the bacterial 16S rRNA gene detected B. thuringiensis at 750 base pairs (bps). The amplified products were sequenced, and the sequence data were confirmed using the Basic Local Alignment Tool (BLAST), which showed a significant alignment (97.98%) with B. thuringiensis and B. cereus. B. thuringiensis were selected to isolate purified crystal proteins (toxins), The protein profile confirmed by SDS-PAGE showed three prominent bands at 70, 36, and 15 kDa. In addition, the larval development of H. contortus was examined in vitro using two different treatments. Purified crystal protein diluted in 10 mM NaCl at a concentration of 2 mg/ml significantly reduced (P < 0.001) larval development by 75.10% compared to 1 × 10 8 CFU/ml spore-crystal suspension reduced (43.97%). The findings of in vitro experiments indicated that purified crystal protein was more toxic to the H. contortus larva than the spore-crystal suspension and control group. Moreover, To test the antinematodal effects of B. thuringiensis toxins in vivo, we chose 12 male goats (6 months old) and reared these animals in parasite-free conditions. We performed Fecal egg count reduction tests (FECRT) on samples collected before and after treatment at various times denotes 48 h post-treatment with Purified crystal proteins was significantly decreased (842 ± 19.07) EPG compared to 24 (2560 ± 233.66) and 12 h (4020 ± 165.22). Similarly, after 48 h of treatment, the FECRT of the Spores-crystal mix was reduced (2920 ± 177.20) EPG followed by 24- and 12-h denotes (4500 ± 137.84) and (4760 ± 112.24), respectively. Results of the above experiment suggested that purified crystal proteins have more anthelmintic potential in vivo. Current findings determine that B. thuringiensis toxin against H. contortus could be used in small ruminants to counter anthelmintic resistance. This study also suggested that future research structured on these proteins' pharmacokinetics and mode of action. (Copyright © 2023 Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Anthelmintic resistance; Bacillus thuringiensis; Biological control; Crystal proteins; Haemonchus contortus; In vitro; In vivo; Spores-crystal suspension |
| المشرفين على المادة: | 0 (RNA, Ribosomal, 16S) 0 (Anthelmintics) 0 (Bacterial Proteins) |
| تواريخ الأحداث: | Date Created: 20230418 Date Completed: 20230606 Latest Revision: 20230606 |
| رمز التحديث: | 20230606 |
| DOI: | 10.1016/j.exppara.2023.108533 |
| PMID: | 37072106 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1090-2449 |
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| DOI: | 10.1016/j.exppara.2023.108533 |