دورية أكاديمية
In vitro Culture and Multilocus Genotyping of Giardia duodenalis Trophozoites Obtained from Human Fecal Samples in Southwest of Iran.
| العنوان: | In vitro Culture and Multilocus Genotyping of Giardia duodenalis Trophozoites Obtained from Human Fecal Samples in Southwest of Iran. |
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| المؤلفون: | Yousefi HA; Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran., Asgarian F; Department of Parasitology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.; School of Allied Medical Sciences, Shahroud University of Medical Sciences, Shahroud, Iran., Tavalla M; Department of Parasitology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.; Department of Parasitology, Infectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran., Ghafari S; Cellular and Molecular Research Center, Faculty of Medicine, Shahrekord University of Medical Sciences, Shahrekord, Iran.; Department of Microbiology and Immunology, School of Medicine, Shahrekord University of Medical Sciences, Shahrekord, Iran., Kohansal K; Department of Parasitology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.; Department of Parasitology, Infectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. |
| المصدر: | Current computer-aided drug design [Curr Comput Aided Drug Des] 2024; Vol. 20 (2), pp. 145-154. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Bentham Science Publishers Country of Publication: United Arab Emirates NLM ID: 101265750 Publication Model: Print Cited Medium: Internet ISSN: 1875-6697 (Electronic) Linking ISSN: 15734099 NLM ISO Abbreviation: Curr Comput Aided Drug Des Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Sharjah, U.A.E. ; San Francisco, CA : Bentham Science Publishers, c2005- |
| مستخلص: | Introduction: The enteric protozoa, Giardia duodenalis (G. duodenalis), consists of eight distinct assemblages (A-H) with identical morphological characteristics and a direct life cycle. Successful axenic cultivation of this parasite is an important preliminary step for biological, drug resistance and phylogenetic studies. Moreover, G. duodenalis exhibits great genetic and biotypic diversity. Aim: The current study aimed to evaluate in vitro culture and multilocus genotyping of G. duodenalis trophozoites obtained from human fecal samples in southwest Iran. Methods: Thirty human fecal specimens containing G. duodenalis cysts were collected from Ahvaz city (southwest of Iran). The purification of cysts was carried out by the sucrose flotation technique. The cysts were inoculated in a modified TYI-S-33 medium and was daily monitored for the development and viability of trophozoites. After extracting DNA, gdh, bg and tpi genes were evaluated using molecular techniques (the semi-nested PCR for gdh gene and the nested PCR for tpi and bg genes). Eventually, the amplified fragments were sequenced and then, the phylogenetic tree was drawn. Results: Of 30, the trophozoites were encysted from five samples. All three genes were detected in two cases of five samples using molecular techniques. The multilocus phylogenetic analysis demonstrated that all the two samples belonged to assemblage A and sub-assemblage AІІ. Conclusion: Our findings indicated the presence of different numbers of trophozoites with variable development and survival rates in modified TYI-S-33 medium. Furthermore, the multilocus genotyping showed that these trophozoites belonged to assemblage A and sub-assemblage AІІ. (Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.) |
| معلومات مُعتمدة: | 3424 Ahvaz Jundishapur University of Medical Sciences |
| فهرسة مساهمة: | Keywords: Axenic cultivation; Giardia duodenalis; TYI-S-33; multilocus genotyping; nested-PCR; trophozoite |
| تواريخ الأحداث: | Date Created: 20230419 Latest Revision: 20231013 |
| رمز التحديث: | 20231013 |
| DOI: | 10.2174/1573409919666230418095958 |
| PMID: | 37076468 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1875-6697 |
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| DOI: | 10.2174/1573409919666230418095958 |