دورية أكاديمية
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Using Hyper as a molecular probe to visualize hydrogen peroxide in living plant cells: An updated method.

التفاصيل البيبلوغرافية
العنوان: Using Hyper as a molecular probe to visualize hydrogen peroxide in living plant cells: An updated method.
المؤلفون: Lara-Rojas F; Centro de Desarrollo de Productos Bióticos, Instituto Politécnico Nacional, Yautepec, Morelos, México., Juárez-Verdayes MA; Departamento de Ciencias Básicas, Universidad Autónoma Agraria Antonio Narro, Saltillo, Coahuila, México., Wu HM; Department of Biochemistry and Molecular Biology, University of Massachusetts, Amherst, MA, United States., Cheung AY; Department of Biochemistry and Molecular Biology, University of Massachusetts, Amherst, MA, United States., Montiel J; Centro de Ciencias Genómicas, Universidad Nacional Autónomas de México. Cuernavaca, Morelos, México., Pascual-Morales E; Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, México., Ryken SE; Department of Biological Sciences, Dartmouth College, Hanover, NH, United States., Bezanilla M; Department of Biological Sciences, Dartmouth College, Hanover, NH, United States., Cardenas L; Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, México. Electronic address: luisc@ibt.unam.mx.
المصدر: Methods in enzymology [Methods Enzymol] 2023; Vol. 683, pp. 265-289. Date of Electronic Publication: 2022 Nov 25.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Academic Press Country of Publication: United States NLM ID: 0212271 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1557-7988 (Electronic) Linking ISSN: 00766879 NLM ISO Abbreviation: Methods Enzymol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: New York, Academic Press.
مواضيع طبية MeSH: Molecular Probes* , Hydrogen Peroxide*/metabolism, Animals ; Reactive Oxygen Species ; Plant Cells/metabolism ; Photosynthesis
مستخلص: Reactive oxygen species (ROS) are highly reactive reduced oxygen molecules that play a myriad of roles in animal and plant cells. In plant cells the production of ROS results from aerobic metabolism during respiration and photosynthesis. Therefore mitochondria, chloroplasts, and peroxisomes constitute an important source of ROS. However, ROS can also be produced in response to many physiological stimuli such as pathogen attack, hormone signaling, abiotic stresses or during cell wall organization and plant morphogenesis. The study of ROS in plant cells has been limited to biochemical assays and use of fluorescent probes, however, the irreversible oxidation of the fluorescent dyes prevents the visualization of dynamic changes. We have previously reported that Hyper 1 is a biosensor for H 2 O 2 and consists of a circularly permutated YFP (cpYFP) inserted into the regulatory domain of the Escherichia coli hydrogen peroxide (H 2 O 2 ) sensor protein OxyR rendering it an H 2 O 2 -specific quantitative probe (Bilan & Belousov, 2018; Hernandez-Barrera et al., 2015). Herein we describe an updated protocol for using the improved new version of Hyper 2 and Hyper 3 as a dynamic biosensor for H 2 O 2 in Arabidopsis with virtually unlimited potential to detect H 2 O 2 throughout the plant and under a broad range of developmental and environmental conditions (Bilan et al., 2013).
(Copyright © 2023 Elsevier Inc. All rights reserved.)
المشرفين على المادة: 0 (Molecular Probes)
BBX060AN9V (Hydrogen Peroxide)
0 (Reactive Oxygen Species)
تواريخ الأحداث: Date Created: 20230422 Date Completed: 20230425 Latest Revision: 20230508
رمز التحديث: 20231215
DOI: 10.1016/bs.mie.2022.09.012
PMID: 37087192
قاعدة البيانات: MEDLINE
الوصف
تدمد:1557-7988
DOI:10.1016/bs.mie.2022.09.012