دورية أكاديمية
Riparin-B as a Potential Inhibitor of AdeABC Efflux System from Acinetobacter baumannii .
العنوان: | Riparin-B as a Potential Inhibitor of AdeABC Efflux System from Acinetobacter baumannii . |
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المؤلفون: | Leão PVS; Laboratory of Research in Microbiology, Department of Parasitology and Microbiology, Federal University of Piaui, Teresina, Piauí, Brazil., Ferreira ALDS; Laboratory of Research in Microbiology, Department of Parasitology and Microbiology, Federal University of Piaui, Teresina, Piauí, Brazil., Oliveira FAA; Laboratory of Research in Microbiology, Department of Parasitology and Microbiology, Federal University of Piaui, Teresina, Piauí, Brazil., Mesquita ABS; Laboratory of Research in Microbiology, Department of Parasitology and Microbiology, Federal University of Piaui, Teresina, Piauí, Brazil., Lima-Net JS; Department of Pharmacy, Federal University of Piauí, Teresina, Piauí, Brazil., Gutierrez SJC; Department of Pharmacy, Federal University of Piauí, Teresina, Piauí, Brazil., Nogueira CES; Department of Biological Chemistry, Regional-University of Cariri, Crato, Ceará, Brazil., Cruz-Martins N; Faculty of Medicine, University of Porto, Alameda Prof. Hernâni Monteiro, Porto 4200-319, Portugal.; Institute for Research and Innovation in Health (i3S), University of Porto, Porto 4200-135, Portugal., Arcanjo DDR; Laboratory of Functional and Molecular Studies on Physiopharmacology (LAFMOL), Department of Biophysics and Physiology, Federal University of Piaui, Teresina, Piauí, Brazil., Barreto HM; Laboratory of Research in Microbiology, Department of Parasitology and Microbiology, Federal University of Piaui, Teresina, Piauí, Brazil., Lima Ferreira JH; Laboratory of Research in Microbiology, Department of Parasitology and Microbiology, Federal University of Piaui, Teresina, Piauí, Brazil. |
المصدر: | Evidence-based complementary and alternative medicine : eCAM [Evid Based Complement Alternat Med] 2023 Apr 08; Vol. 2023, pp. 1780838. Date of Electronic Publication: 2023 Apr 08 (Print Publication: 2023). |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Hindawi Pub Country of Publication: United States NLM ID: 101215021 Publication Model: eCollection Cited Medium: Print ISSN: 1741-427X (Print) Linking ISSN: 1741427X NLM ISO Abbreviation: Evid Based Complement Alternat Med Subsets: PubMed not MEDLINE |
أسماء مطبوعة: | Publication: 2011- : New York, NY : Hindawi Pub. Original Publication: Oxford, UK : Oxford University Press, c2004- |
مستخلص: | Acinetobacter baumannii is an important opportunistic pathogen that causes serious health-related infections, especially in intensive care units. The present study aimed to investigate the antimicrobial activity of Riparin-B (Rip-B) alone and in association with norfloxacin against multidrug-resistant clinical isolates of A. baumannii . For this, the minimum inhibitory concentrations were determined by the microdilution method. For the evaluation of resistance-modulating activity, MIC values for antibiotics were determined in the presence or absence of subinhibitory concentrations of Rip-B or chlorpromazine (CPZ). The AdeABC-AdeRS efflux system genes from these isolates were detected by PCR. Docking studies were also carried out to evaluate the interaction of Riparin-B and the AdeABC-AdeRS efflux system. The study was conducted from 2017 to 2019. The results showed that Rip-B showed weak intrinsic activity against the strains tested. On the other hand, Rip-B was able to modulate norfloxacin's response against A. baumannii strains that express efflux pump-mediated resistance. Docking studies provided projections of the interaction between Rip-B and EtBr with the AdeB protein, suggesting that Rip-B acts by competitive inhibition with the drug. Results found by in vitro and in silico assays suggest that Rip-B, in combination with norfloxacin, has the potential to treat infections caused by multidrug-resistant A. baumanni with efflux pump resistance. Competing Interests: The authors declare no conflicts of interest. (Copyright © 2023 Patrícia Virna Sales Leão et al.) |
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تواريخ الأحداث: | Date Created: 20230424 Latest Revision: 20230425 |
رمز التحديث: | 20230425 |
مُعرف محوري في PubMed: | PMC10118900 |
DOI: | 10.1155/2023/1780838 |
PMID: | 37089710 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1741-427X |
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DOI: | 10.1155/2023/1780838 |