دورية أكاديمية
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A novel method to estimate biofilm activity based on enzymatic oxygen release from hydrogen peroxide decomposition.

التفاصيل البيبلوغرافية
العنوان: A novel method to estimate biofilm activity based on enzymatic oxygen release from hydrogen peroxide decomposition.
المؤلفون: Qi W; Technical University of Denmark, DTU Aqua, Section for Aquaculture, The North Sea Research Centre, P.O. Box 101, DK-9850, Hirtshals, Denmark., Skov PV; Technical University of Denmark, DTU Aqua, Section for Aquaculture, The North Sea Research Centre, P.O. Box 101, DK-9850, Hirtshals, Denmark., de Jesus Gregersen KJ; Technical University of Denmark, DTU Aqua, Section for Aquaculture, The North Sea Research Centre, P.O. Box 101, DK-9850, Hirtshals, Denmark., Pedersen LF; Technical University of Denmark, DTU Aqua, Section for Aquaculture, The North Sea Research Centre, P.O. Box 101, DK-9850, Hirtshals, Denmark.
المصدر: Biofilm [Biofilm] 2023 Apr 07; Vol. 5, pp. 100121. Date of Electronic Publication: 2023 Apr 07 (Print Publication: 2023).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier B.V Country of Publication: Netherlands NLM ID: 101764202 Publication Model: eCollection Cited Medium: Internet ISSN: 2590-2075 (Electronic) Linking ISSN: 25902075 NLM ISO Abbreviation: Biofilm Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: [Amsterdam] : Elsevier B.V., [2019]-
مستخلص: Biofilm is central for biological water treatment processes in recirculating aquaculture systems (RAS). A lack of suitable methods for quantifying biofilm activity, however, makes it difficult to assess and compare the microbial status of biofilm. This type of information of the biofilm will be useful to assess the colonization status of nitrifying biocarriers or to evaluate the effect of disinfectants on the biofilm activity. Here we introduce a novel assay for rapid assessment of microbial activities in the biofilm attached on bioelements from a RAS biofilter. The assay consisted of an intermittent respirometer platform where biofilter elements were exposed to 10 mg/L hydrogen peroxide (H 2 O 2 ) for 1 h, following concurrent measurements of oxygen release from the decomposition of H 2 O 2 caused by biofilm-associated enzymes. A different number of colonized, mature bioelements from a moving bed biofilter in a freshwater RAS were tested with repeated H 2 O 2 exposure, and compared against their autoclaved forms. A substantial increase in dissolved oxygen (DO) concentration (0.92-2.31 mg O 2 /L) occurred with mature bioelements during 1 h of H 2 O 2 exposure, compared to small amounts of DO release (≤0.27 mg O 2 /L) with autoclaved bioelements. This substantiates that H 2 O 2 decomposition by biofilm is mainly governed by microbial enzymatic activities. A monomolecular model fitted well with the observed oxygen release profiles of tested mature bioelements after H 2 O 2 exposure (R 2  > 0.98). The kinetic rate constant of net oxygen release ( k or , h -1 ) was proportional (R 2 for linear fit = 0.99) to the number of mature bioelements tested. Repeated exposure of H 2 O 2 to the same bioelements did not change k or , which indicates that 10 mg/L H 2 O 2 with an exposure time of 1 h does not suppress enzymatic activity in biofilm. Our study provides a new rapid method that allows simple quantification of microbial activity in biofilm samples from aquaculture systems, which could potentially be also applied to study biofilm from wastewater treatment plants and other industries.
Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(©2023PublishedbyElsevierB.V.)
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فهرسة مساهمة: Keywords: Biofilm activity; Biofilter; Disinfection; H2O2 decomposition; Recirculating aquaculture system; Respirometry
تواريخ الأحداث: Date Created: 20230424 Latest Revision: 20230425
رمز التحديث: 20230425
مُعرف محوري في PubMed: PMC10119708
DOI: 10.1016/j.bioflm.2023.100121
PMID: 37090160
قاعدة البيانات: MEDLINE
الوصف
تدمد:2590-2075
DOI:10.1016/j.bioflm.2023.100121