دورية أكاديمية

Recovery of dried samples from the Helminthological Collection of the Oswaldo Cruz Institute (CHIOC)/Fiocruz through specimen rehydration technique.

التفاصيل البيبلوغرافية
العنوان: Recovery of dried samples from the Helminthological Collection of the Oswaldo Cruz Institute (CHIOC)/Fiocruz through specimen rehydration technique.
المؤلفون: Mainenti A; Laboratório de Helmintos Parasitos de Vertebrados, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil.; Laboratório de Malacologia Médica, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil., Lopes DA; Laboratório de Helmintos Parasitos de Vertebrados, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil., Cruz AGD; Laboratório de Helmintos Parasitos de Vertebrados, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil., Souza PVR; Instituto de Tecnologia em Fármacos, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil., Fonseca MCGD; Laboratório de Helmintos Parasitos de Vertebrados, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil., Fernandez MA; Laboratório de Malacologia Médica, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil., Gomes DC; Laboratório de Helmintos Parasitos de Vertebrados, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil., Knoff M; Laboratório de Helmintos Parasitos de Vertebrados, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil.
المصدر: Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria [Rev Bras Parasitol Vet] 2023 Apr 21; Vol. 32 (2), pp. e000123. Date of Electronic Publication: 2023 Apr 21 (Print Publication: 2023).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Colégio Brasileiro de Parasitologia Veterinária Country of Publication: Brazil NLM ID: 9440482 Publication Model: eCollection Cited Medium: Internet ISSN: 1984-2961 (Electronic) Linking ISSN: 0103846X NLM ISO Abbreviation: Rev Bras Parasitol Vet Subsets: MEDLINE
أسماء مطبوعة: Original Publication: São Paulo, Brasil : Colégio Brasileiro de Parasitologia Veterinária, [1992]-
مواضيع طبية MeSH: Acanthocephala* , Helminths* , Nematoda* , Platyhelminths* , Trematoda*, Animals
مستخلص: The Helminthological Collection of the Oswaldo Cruz Institute is the biggest in Latin America and it is among the largest collections at worldwide reference level, with around 40,000 sets of specimens and approximately one million individual specimens. It contains helminths parasites of vertebrate and invertebrate animals that form part of the fauna of Brazil and other countries. The samples comprise holotypes, paratypes and representative specimens of Platyhelminthes, Acanthocephala, Nematoda and other non-helminth phyla, such as Annelida and Arthropoda. Some of the samples preserved in liquid media were found to have dried out. This made it impossible to analyze these samples morphologically for taxonomic purposes. The aim of this study was to test techniques used for rehydration of the tegument of specimens that had been found to have dried out and present protocols for such techniques. A total of 528 specimens that either no longer were immersed in preservatives or had already dried out were analyzed: 96 digenetic trematodes, 45 cestodes, 22 acanthocephalans, 357 nematodes, four hirudineans and four pentastomid crustaceans. The technique of rehydration using only distilled water on the specimens proved to be efficient for recovering tegument malleability, for all samples analyzed in this present study.
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تواريخ الأحداث: Date Created: 20230426 Date Completed: 20230428 Latest Revision: 20230428
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC10124609
DOI: 10.1590/S1984-29612023021
PMID: 37098919
قاعدة البيانات: MEDLINE
الوصف
تدمد:1984-2961
DOI:10.1590/S1984-29612023021