دورية أكاديمية
Behavioural, genomics and proteomic approach to examine Alzheimer's disease in zebrafish.
| العنوان: | Behavioural, genomics and proteomic approach to examine Alzheimer's disease in zebrafish. |
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| المؤلفون: | Siddiqui A; Neurological disorder and aging research group (NDA), Neuroscience Research Strength (NRS), Jeffrey Cheah School of Medicine and Health Sciences, Monash University Malaysia, 47500, Selangor, Malaysia., Abidin SAZ; Jeffrey Cheah School of Medicine and Health Sciences, Monash University Malaysia, 47500, Selangor, Malaysia., Shah ZA; Department of Medicinal and Biological Chemistry, University of Toledo, 43614, 3000 Arlington Avenue, Toledo, OH, USA., Othman I; Jeffrey Cheah School of Medicine and Health Sciences, Monash University Malaysia, 47500, Selangor, Malaysia., Kumari Y; Neurological disorder and aging research group (NDA), Neuroscience Research Strength (NRS), Jeffrey Cheah School of Medicine and Health Sciences, Monash University Malaysia, 47500, Selangor, Malaysia. Electronic address: yatinesh.kumari@monash.edu. |
| المصدر: | Comparative biochemistry and physiology. Toxicology & pharmacology : CBP [Comp Biochem Physiol C Toxicol Pharmacol] 2023 Sep; Vol. 271, pp. 109636. Date of Electronic Publication: 2023 Apr 24. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Science Country of Publication: United States NLM ID: 100959500 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1532-0456 (Print) Linking ISSN: 15320456 NLM ISO Abbreviation: Comp Biochem Physiol C Toxicol Pharmacol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: New York, NY : Elsevier Science, c2000- |
| مواضيع طبية MeSH: | Alzheimer Disease*/genetics , Alzheimer Disease*/drug therapy , Alzheimer Disease*/metabolism, Aged ; Animals ; Humans ; Zebrafish/metabolism ; Proteomics ; Actins/metabolism ; Brain/metabolism ; Okadaic Acid/adverse effects ; Okadaic Acid/metabolism ; Genomics ; Disease Models, Animal |
| مستخلص: | Globally around 24 million elderly population are dealing with dementia, and this pathological characteristic is commonly seen in people suffering from Alzheimer's disease (AD). Despite having multiple treatment options that can mitigate AD symptoms, there is an imperative call to advance our understanding of the disease pathogenesis to unfold disease-modifying treatments/therapies. To explore the driving mechanisms of AD development, we stretch out further to study time-dependant changes after Okadaic acid (OKA)-induced AD-like conditions in zebrafish. We evaluated the pharmacodynamics of OKA at two-time points, i.e., after 4-days and 10-days exposure to zebrafish. T-Maze was utilized to observe the learning and cognitive behaviour, and inflammatory gene expressions such as 5-Lox, Gfap, Actin, APP, and Mapt were performed in zebrafish brains. To scoop everything out from the brain tissue, protein profiling was performed using LCMS/MS. Both time course OKA-induced AD models have shown significant memory impairment, as evident from T-Maze. Gene expression studies of both groups have reported an overexpression of 5-Lox, GFAP, Actin, APP, and OKA 10D group has shown remarkable upregulation of Mapt in zebrafish brains. In the case of protein expression, the heatmap suggested an important role of some common proteins identified in both groups, which can be explored further to investigate their mechanism in OKA-induced AD pathology. Presently, the preclinical models available to understand AD-like conditions are not completely understood. Hence, utilizing OKA in the zebrafish model can be of great importance in understanding the pathology of AD progression and as a screening tool for drug discovery. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Alzheimer's diseases; Neurodegeneration; Neuroinflammation; Okadaic acid; Zebrafish |
| المشرفين على المادة: | 0 (Actins) 1W21G5Q4N2 (Okadaic Acid) |
| تواريخ الأحداث: | Date Created: 20230426 Date Completed: 20230731 Latest Revision: 20230731 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1016/j.cbpc.2023.109636 |
| PMID: | 37100105 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1532-0456 |
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| DOI: | 10.1016/j.cbpc.2023.109636 |