دورية أكاديمية
أعرض في EDS

Full confluency, serum starvation, and roscovitine for inducing arrest in the G 0 /G 1 phase of the cell cycle in puma skin-derived fibroblast lines.

التفاصيل البيبلوغرافية
العنوان: Full confluency, serum starvation, and roscovitine for inducing arrest in the G 0 /G 1 phase of the cell cycle in puma skin-derived fibroblast lines.
المؤلفون: Rodrigues LLV; Laboratório de Biotecnologia Animal, Universidade Federal Rural do Semi-Árido, Mossoró, RN, Brasil., Moura YBF; Laboratório de Biotecnologia Animal, Universidade Federal Rural do Semi-Árido, Mossoró, RN, Brasil., Viana JVDS; Laboratório de Biotecnologia Animal, Universidade Federal Rural do Semi-Árido, Mossoró, RN, Brasil., de Oliveira LRM; Laboratório de Biotecnologia Animal, Universidade Federal Rural do Semi-Árido, Mossoró, RN, Brasil., Praxedes ÉA; Laboratório de Biotecnologia Animal, Universidade Federal Rural do Semi-Árido, Mossoró, RN, Brasil., Vieira JB; Laboratório de Oncologia Experimental, Universidade Federal do Ceará, Fortaleza, CE, Brasil., Sales SLA; Laboratório de Oncologia Experimental, Universidade Federal do Ceará, Fortaleza, CE, Brasil., Silva HVR; Laboratório de Reprodução de Carnívoros, Universidade Estadual do Ceará, Fortaleza, CE, Brasil., Luciano MCDS; Laboratório de Oncologia Experimental, Universidade Federal do Ceará, Fortaleza, CE, Brasil., Pessoa C; Laboratório de Oncologia Experimental, Universidade Federal do Ceará, Fortaleza, CE, Brasil., Pereira AF; Laboratório de Biotecnologia Animal, Universidade Federal Rural do Semi-Árido, Mossoró, RN, Brasil.
المصدر: Animal reproduction [Anim Reprod] 2023 Apr 21; Vol. 20 (1), pp. e20230017. Date of Electronic Publication: 2023 Apr 21 (Print Publication: 2023).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Colégio Brasileiro de Reprodução Animal Country of Publication: Brazil NLM ID: 101272804 Publication Model: eCollection Cited Medium: Internet ISSN: 1984-3143 (Electronic) Linking ISSN: 18069614 NLM ISO Abbreviation: Anim Reprod Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Belo Horizonte, MG, Brazil : Colégio Brasileiro de Reprodução Animal, [2004]-
مستخلص: The puma population is constantly decreasing, and cloning by somatic cell nuclear transfer can be used to conserve the species. One of the factors determining the success of the development of cloned embryos is the cell cycle stage of the donor cells. We evaluated the effects of full confluency (~100%), serum starvation (0.5% serum), and roscovitine (15 µM) treatments on the cell cycle synchronization in G 0 /G 1 of puma skin-derived fibroblasts by flow cytometric analysis. Also, we assessed the effects of these synchronization methods on morphology, viability, and apoptosis levels using microscopy tools. The results showed that culturing the cells to confluence for 24 h (84.0%), 48 h (84.6%), and 72 h (84.2%) and serum starvation for 96 h (85.4%) yielded a significantly higher percentage of cells arrested in the G 0 /G 1 (P 0.05) phase than cells not subjected to any cell cycle synchronization method (73.9%). Nevertheless, while serum starvation reduced the percentage of viable cells, no difference was observed for the full confluence and roscovitine treatments (P 0.05). Moreover, roscovitine for 12 h (78.6%) and 24 h (82.1%) was unable to synchronize cells in G 0 /G 1 (P 0.05). In summary, full confluency induces puma fibroblast cell cycle synchronization at the G 0 /G 1 stage without affecting cell viability. These outcomes may be valuable for planning donor cells for somatic cell nuclear transfer in pumas.
Competing Interests: Conflicts of interest: The authors have no conflict of interest to declare.
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فهرسة مساهمة: Keywords: cell cycle synchronization; culture to confluence; felids; somatic cell nuclear transfer
تواريخ الأحداث: Date Created: 20230427 Latest Revision: 20230428
رمز التحديث: 20230428
مُعرف محوري في PubMed: PMC10124155
DOI: 10.1590/1984-3143-AR2023-0017
PMID: 37101424
قاعدة البيانات: MEDLINE
الوصف
تدمد:1984-3143
DOI:10.1590/1984-3143-AR2023-0017