دورية أكاديمية
Vascular cells improve functionality of human cardiac organoids.
| العنوان: | Vascular cells improve functionality of human cardiac organoids. |
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| المؤلفون: | Voges HK; QIMR Berghofer Medical Research Institute, Brisbane, QLD 4006, Australia; School of Biomedical Sciences, The University of Queensland, Brisbane, QLD 4072, Australia; Murdoch Children's Research Institute, The Royal Children's Hospital, Melbourne, VIC 3052, Australia; Department of Paediatrics, School of Medicine, Dentistry and Health Sciences, The University of Melbourne, Melbourne, VIC 3052, Australia; Novo Nordisk Foundation Center for Stem Cell Medicine, Murdoch Children's Research Institute, Melbourne, VIC 3052, Australia., Foster SR; QIMR Berghofer Medical Research Institute, Brisbane, QLD 4006, Australia., Reynolds L; QIMR Berghofer Medical Research Institute, Brisbane, QLD 4006, Australia., Parker BL; Charles Perkins Centre, School of Life and Environmental Science, The University of Sydney, Sydney, NSW 2006, Australia; Department of Anatomy and Physiology, School of Biomedical Sciences, The University of Melbourne, Melbourne, VIC 3052, Australia., Devilée L; QIMR Berghofer Medical Research Institute, Brisbane, QLD 4006, Australia., Quaife-Ryan GA; QIMR Berghofer Medical Research Institute, Brisbane, QLD 4006, Australia; School of Biomedical Sciences, The University of Queensland, Brisbane, QLD 4072, Australia., Fortuna PRJ; QIMR Berghofer Medical Research Institute, Brisbane, QLD 4006, Australia., Mathieson E; QIMR Berghofer Medical Research Institute, Brisbane, QLD 4006, Australia., Fitzsimmons R; QIMR Berghofer Medical Research Institute, Brisbane, QLD 4006, Australia., Lor M; QIMR Berghofer Medical Research Institute, Brisbane, QLD 4006, Australia., Batho C; QIMR Berghofer Medical Research Institute, Brisbane, QLD 4006, Australia., Reid J; QIMR Berghofer Medical Research Institute, Brisbane, QLD 4006, Australia; School of Biomedical Sciences, The University of Queensland, Brisbane, QLD 4072, Australia., Pocock M; QIMR Berghofer Medical Research Institute, Brisbane, QLD 4006, Australia., Friedman CE; Institute for Molecular Bioscience, University of Queensland, Brisbane 4072, QLD, Australia., Mizikovsky D; Institute for Molecular Bioscience, University of Queensland, Brisbane 4072, QLD, Australia., Francois M; The Centenary Institute, David Richmond Program for Cardiovascular Research: Gene Regulation and Editing, Sydney Medical School, University of Sydney, Sydney, NSW 2050, Australia., Palpant NJ; Institute for Molecular Bioscience, University of Queensland, Brisbane 4072, QLD, Australia., Needham EJ; Charles Perkins Centre, School of Life and Environmental Science, The University of Sydney, Sydney, NSW 2006, Australia., Peralta M; Australian Regenerative Medicine Institute. Monash University, Clayton, VIC 3800, Australia., Monte-Nieto GD; Australian Regenerative Medicine Institute. Monash University, Clayton, VIC 3800, Australia., Jones LK; Department of Anatomy and Developmental Biology, Development and Stem Cells Program, Monash Biomedical Discovery Institute, Monash University, Melbourne, VIC 3800, Australia., Smyth IM; Department of Anatomy and Developmental Biology, Development and Stem Cells Program, Monash Biomedical Discovery Institute, Monash University, Melbourne, VIC 3800, Australia., Mehdiabadi NR; Murdoch Children's Research Institute, The Royal Children's Hospital, Melbourne, VIC 3052, Australia; Novo Nordisk Foundation Center for Stem Cell Medicine, Murdoch Children's Research Institute, Melbourne, VIC 3052, Australia., Bolk F; Murdoch Children's Research Institute, The Royal Children's Hospital, Melbourne, VIC 3052, Australia; Novo Nordisk Foundation Center for Stem Cell Medicine, Murdoch Children's Research Institute, Melbourne, VIC 3052, Australia., Janbandhu V; Victor Chang Cardiac Research Institute, Sydney, NSW 2010, Australia; School of Clinical Medicine, Faculty of Medicine and Health, UNSW Sydney, Sydney, NSW 2052, Australia., Yao E; Victor Chang Cardiac Research Institute, Sydney, NSW 2010, Australia., Harvey RP; Victor Chang Cardiac Research Institute, Sydney, NSW 2010, Australia; School of Clinical Medicine, Faculty of Medicine and Health, UNSW Sydney, Sydney, NSW 2052, Australia; School of Biotechnology and Biomolecular Science, UNSW Sydney, Sydney, NSW 2052, Australia., Chong JJH; Centre for Heart Research, Westmead Institute for Medical Research, The University of Sydney, Sydney, NSW 2145, Australia; Department of Cardiology, Westmead Hospital, Westmead, NSW 2145, Australia., Elliott DA; Murdoch Children's Research Institute, The Royal Children's Hospital, Melbourne, VIC 3052, Australia; Department of Paediatrics, School of Medicine, Dentistry and Health Sciences, The University of Melbourne, Melbourne, VIC 3052, Australia; Novo Nordisk Foundation Center for Stem Cell Medicine, Murdoch Children's Research Institute, Melbourne, VIC 3052, Australia., Stanley EG; Murdoch Children's Research Institute, The Royal Children's Hospital, Melbourne, VIC 3052, Australia; Department of Paediatrics, School of Medicine, Dentistry and Health Sciences, The University of Melbourne, Melbourne, VIC 3052, Australia; Novo Nordisk Foundation Center for Stem Cell Medicine, Murdoch Children's Research Institute, Melbourne, VIC 3052, Australia., Wiszniak S; Centre for Cancer Biology, SA Pathology and University of South Australia, Adelaide, SA 5001, Australia., Schwarz Q; Centre for Cancer Biology, SA Pathology and University of South Australia, Adelaide, SA 5001, Australia., James DE; Charles Perkins Centre, School of Life and Environmental Science, The University of Sydney, Sydney, NSW 2006, Australia; Sydney Medical School, The University of Sydney, Sydney, 2010 NSW, Australia., Mills RJ; QIMR Berghofer Medical Research Institute, Brisbane, QLD 4006, Australia; School of Biomedical Sciences, The University of Queensland, Brisbane, QLD 4072, Australia; Murdoch Children's Research Institute, The Royal Children's Hospital, Melbourne, VIC 3052, Australia; Department of Paediatrics, School of Medicine, Dentistry and Health Sciences, The University of Melbourne, Melbourne, VIC 3052, Australia; Novo Nordisk Foundation Center for Stem Cell Medicine, Murdoch Children's Research Institute, Melbourne, VIC 3052, Australia., Porrello ER; Murdoch Children's Research Institute, The Royal Children's Hospital, Melbourne, VIC 3052, Australia; Novo Nordisk Foundation Center for Stem Cell Medicine, Murdoch Children's Research Institute, Melbourne, VIC 3052, Australia; Department of Anatomy and Physiology, School of Biomedical Sciences, The University of Melbourne, Melbourne, VIC 3052, Australia; Melbourne Centre for Cardiovascular Genomics and Regenerative Medicine, The Royal Children's Hospital, Melbourne, VIC 3052, Australia. Electronic address: enzo.porrello@mcri.edu.au., Hudson JE; QIMR Berghofer Medical Research Institute, Brisbane, QLD 4006, Australia; School of Biomedical Sciences, The University of Queensland, Brisbane, QLD 4072, Australia. Electronic address: james.hudson@qimrberghofer.edu.au. |
| المصدر: | Cell reports [Cell Rep] 2023 May 30; Vol. 42 (5), pp. 112322. Date of Electronic Publication: 2023 Apr 26. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Cell Press Country of Publication: United States NLM ID: 101573691 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2211-1247 (Electronic) NLM ISO Abbreviation: Cell Rep Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: [Cambridge, MA] : Cell Press, c 2012- |
| مواضيع طبية MeSH: | Endothelial Cells* , Myocytes, Cardiac*/metabolism, Humans ; Pericytes/metabolism ; Signal Transduction ; Organoids/metabolism |
| مستخلص: | Crosstalk between cardiac cells is critical for heart performance. Here we show that vascular cells within human cardiac organoids (hCOs) enhance their maturation, force of contraction, and utility in disease modeling. Herein we optimize our protocol to generate vascular populations in addition to epicardial, fibroblast, and cardiomyocyte cells that self-organize into in-vivo-like structures in hCOs. We identify mechanisms of communication between endothelial cells, pericytes, fibroblasts, and cardiomyocytes that ultimately contribute to cardiac organoid maturation. In particular, (1) endothelial-derived LAMA5 regulates expression of mature sarcomeric proteins and contractility, and (2) paracrine platelet-derived growth factor receptor β (PDGFRβ) signaling from vascular cells upregulates matrix deposition to augment hCO contractile force. Finally, we demonstrate that vascular cells determine the magnitude of diastolic dysfunction caused by inflammatory factors and identify a paracrine role of endothelin driving dysfunction. Together this study highlights the importance and role of vascular cells in organoid models. Competing Interests: Declaration of interests R.J.M., J.E.H., G.A.Q.-R., and E.R.P. are listed as co-inventors on pending patents that relate to cardiac organoid maturation and cardiac regeneration therapeutics. E.R.P. and J.E.H. are listed as co-inventors on pending patents that relate to endothelial cells in 3D cardiac products. J.E.H. is a co-inventor on licensed patents relating to engineered heart muscle. R.J.M., E.R.P., and J.E.H. are co-founders, scientific advisors, and stockholders in Dynomics. (Copyright © 2023 The Author(s). Published by Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: CP: Stem cell research; LAMA5; PDGF; cardiac organoid; disease modeling; endothelial cells; endothelin-1; extracellular matrix; inflammation |
| تواريخ الأحداث: | Date Created: 20230427 Date Completed: 20230605 Latest Revision: 20230605 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/j.celrep.2023.112322 |
| PMID: | 37105170 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2211-1247 |
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| DOI: | 10.1016/j.celrep.2023.112322 |