دورية أكاديمية
Genetic diversity of the oipA gene among Helicobacter pylori isolates and clinical outcome in Vietnam.
| العنوان: | Genetic diversity of the oipA gene among Helicobacter pylori isolates and clinical outcome in Vietnam. |
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| المؤلفون: | Thai THN; Department of Internal Medicine, University of Medicine and Pharmacy, Hue University, Hue, Viet Nam; Department of Internal Medicine, Faculty of Medicine, Can Tho University of Medicine and Pharmacy, Can Tho, Viet Nam., Nguyen HP; Department of Pathology and Forensic Medicine, Faculty of Medicine, Can Tho University of Medicine and Pharmacy, Can Tho, Viet Nam., Nguyen THY; Department of Microbiology, Faculty of Medicine, Can Tho University of Medicine and Pharmacy, Can Tho, Viet Nam., Nguyen TBH; Department of Microbiology, Faculty of Medicine, Can Tho University of Medicine and Pharmacy, Can Tho, Viet Nam., Nguyen TH; Department of Internal Medicine, Faculty of Medicine, Can Tho University of Medicine and Pharmacy, Can Tho, Viet Nam., Nguyen TMN; Department of Medical Genetics, University of Medicine and Pharmacy, Hue University, Hue, Viet Nam., Ha TMT; Department of Medical Genetics, University of Medicine and Pharmacy, Hue University, Hue, Viet Nam; Institute of Biomedicine, University of Medicine and Pharmacy, Hue University, Hue, Viet Nam. Electronic address: htmthi@hueuni.edu.vn. |
| المصدر: | Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases [Infect Genet Evol] 2023 Aug; Vol. 112, pp. 105438. Date of Electronic Publication: 2023 Apr 25. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Science Country of Publication: Netherlands NLM ID: 101084138 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1567-7257 (Electronic) Linking ISSN: 15671348 NLM ISO Abbreviation: Infect Genet Evol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Amsterdam ; New York : Elsevier Science, c2001- |
| مواضيع طبية MeSH: | Helicobacter pylori*/genetics , Helicobacter pylori*/metabolism , Peptic Ulcer*/pathology , Gastritis* , Helicobacter Infections*/epidemiology , Helicobacter Infections*/pathology, Humans ; Bacterial Outer Membrane Proteins/genetics ; Cross-Sectional Studies ; Vietnam/epidemiology ; Genetic Variation ; Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; Antigens, Bacterial/genetics |
| مستخلص: | Outer inflammatory protein A (OipA), which is encoded by the oipA gene, can induce interleukin-8 secretion in gastric epithelial cells. The functional status of the oipA gene is regulated by the slipped-strand mispairing mechanism based on the CT dinucleotide repeat number in the 5' region. This study aimed to investigate the oipA functional status ("on/off") of Helicobacter pylori (H. pylori) and its association with gastroduodenal diseases in southwestern Vietnam. The cross-sectional study was conducted on 173H. pylori isolates from 173 patients with gastroduodenal diseases. Sanger sequencing was used to determine the functional status of oipA. Multivariable logistic regression analysis was performed to identify the association between oipA status and gastroduodenal diseases. The oipA "on" status accounted for 96% of H. pylori isolates. Twenty-five CT repeat patterns of the oipA 5' signal region were observed, five of which were novel CT repeat patterns. The oipA "on" status was found in 100%, 97.8%, and 86.8% of H. pylori isolates from patients with peptic ulcer, precancerous lesions, and chronic gastritis, respectively (p < 0.01). The oipA "on" status was related to gastric precancerous lesions versus chronic gastritis (adjusted OR = 7.39, 95% CI: 1.35-40.59, p = 0.021) and peptic ulcers versus chronic gastritis (adjusted OR = 12.79, 95% CI: 1.19-1760.32, p = 0.033). Our data show a high prevalence of the oipA "on" status, which was associated with precancerous gastric lesions and peptic ulcers. Moreover, genetic diversity in the number and pattern of CT dinucleotide repeat of oipA among Vietnamese H. pylori strains was identified. Competing Interests: Declaration of Competing Interest The authors declare no conflict of interest. (Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Gastroduodenal diseases; Genetic diversity; Helicobacter pylori; oipA; “On/off” status |
| المشرفين على المادة: | 0 (Bacterial Outer Membrane Proteins) 0 (Bacterial Proteins) 0 (Antigens, Bacterial) |
| تواريخ الأحداث: | Date Created: 20230427 Date Completed: 20230621 Latest Revision: 20230621 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1016/j.meegid.2023.105438 |
| PMID: | 37105346 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1567-7257 |
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| DOI: | 10.1016/j.meegid.2023.105438 |