دورية أكاديمية
L-carnitine enhances developmental potential of bovine oocytes matured under high lipid concentrations in vitro.
| العنوان: | L-carnitine enhances developmental potential of bovine oocytes matured under high lipid concentrations in vitro. |
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| المؤلفون: | Catandi GD; Equine Reproduction Laboratory, Department of Biomedical Sciences, Colorado State University, 3101 Rampart Road, Fort Collins, CO 80521, USA; Department of Biomedical Sciences, Colorado State University, Fort Collins, CO 80523, USA., Cheng MH; Department of Electrical and Computer Engineering, Colorado State University, Fort Collins, CO 80523, USA., Chicco AJ; Department of Biomedical Sciences, Colorado State University, Fort Collins, CO 80523, USA., Chen T; Department of Electrical and Computer Engineering, Colorado State University, Fort Collins, CO 80523, USA; School of Biomedical Engineering, Colorado State University, Fort Collins, CO 80523, USA., Carnevale EM; Equine Reproduction Laboratory, Department of Biomedical Sciences, Colorado State University, 3101 Rampart Road, Fort Collins, CO 80521, USA; Department of Biomedical Sciences, Colorado State University, Fort Collins, CO 80523, USA. Electronic address: elaine.carnevale@colostate.edu. |
| المصدر: | Animal reproduction science [Anim Reprod Sci] 2023 May; Vol. 252, pp. 107249. Date of Electronic Publication: 2023 Apr 25. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Scientific Pub. Co Country of Publication: Netherlands NLM ID: 7807205 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-2232 (Electronic) Linking ISSN: 03784320 NLM ISO Abbreviation: Anim Reprod Sci Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Amsterdam, Elsevier Scientific Pub. Co. |
| مواضيع طبية MeSH: | In Vitro Oocyte Maturation Techniques*/veterinary , Carnitine*/pharmacology , Carnitine*/metabolism, Animals ; Cattle ; Female ; Pregnancy ; Fatty Acids, Nonesterified/pharmacology ; Fatty Acids, Nonesterified/metabolism ; Reactive Oxygen Species/metabolism ; Oocytes ; Blastocyst ; Embryonic Development |
| مستخلص: | Maternal obesity elevates non-esterified fatty acids (NEFA) follicular concentrations. Bovine cumulus-oocyte complexes (COCs) matured in vitro under high NEFA have altered metabolism and reduced quality. Systemically, obesity promotes altered mitochondrial metabolism linked to L-carnitine insufficiency. We hypothesized that L-carnitine supplementation during IVM of bovine COCs in the presence of high NEFA would lessen the negative effects of exposure to excessive lipids on embryonic development and oxidative stress. COCs were collected from abattoir ovaries and matured in four groups: CON (control), LC (3 mM L-carnitine), HN (high NEFA: 200uM oleic, 150uM palmitic and 75uM stearic acid), and HNLC (HN and LC). Mature oocytes were assayed for aerobic and anaerobic metabolism utilizing oxygen and pH microsensors or fertilized in vitro (D0). Cleavage (D3) and blastocyst (D7, D8) rates were assessed. D3 embryos with ≥ 4 cells were stained for cytosolic and mitochondrial ROS. D8 blastocysts were assayed for gene transcript abundance of metabolic enzymes. Oocyte metabolism was not affected by IVM treatment. D3 formation of embryos with ≥ 4 cells were lower in LC or HN than CON or HNLC; blastocyst rates were greater for CON and lower for HN than LC and HNLC. D3 embryo mitochondrial and cytosolic ROS were reduced in HNLC when compared to other groups. IVM in HN altered blastocyst gene transcript abundance when compared to CON, but not LC or HNLC. In conclusion, supplementation with L-carnitine protects oocytes exposed to high NEFA during IVM and improves their developmental competence, suggesting that high lipid exposure may lead to L-carnitine insufficiency in bovine oocytes. Competing Interests: Declaration of Competing Interest The authors declare no conflict of interest that could have influenced the work reported in this article. (Copyright © 2023 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Antioxidant; Embryo; In vitro fertilization; Metabolism; Mitochondria; Non-esterified fatty acids |
| المشرفين على المادة: | S7UI8SM58A (Carnitine) 0 (Fatty Acids, Nonesterified) 0 (Reactive Oxygen Species) |
| تواريخ الأحداث: | Date Created: 20230429 Date Completed: 20230522 Latest Revision: 20230522 |
| رمز التحديث: | 20230522 |
| DOI: | 10.1016/j.anireprosci.2023.107249 |
| PMID: | 37119563 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-2232 |
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| DOI: | 10.1016/j.anireprosci.2023.107249 |