دورية أكاديمية
أعرض في EDS

Live attenuated Leishmania infantum centrin deleted mutant (LiCen -/- ) as a novel vaccine candidate: A field study on safety, immunogenicity, and efficacy against canine leishmaniasis.

التفاصيل البيبلوغرافية
العنوان: Live attenuated Leishmania infantum centrin deleted mutant (LiCen -/- ) as a novel vaccine candidate: A field study on safety, immunogenicity, and efficacy against canine leishmaniasis.
المؤلفون: Zarei Z; Department of Pathobiology, School Veterinary Medicine, Ferdowsi University of Mashhad, P.O. Box 91775-1793, Mashhad, Iran; Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, P.O. Box 14155-6446, Tehran, Iran., Mohebali M; Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, P.O. Box 14155-6446, Tehran, Iran; Center for Research of Endemic Parasites of Iran (CREPI), Tehran University of Medical Sciences, Tehran, Iran. Electronic address: mohebali@tums.ac.ir., Dehghani H; Department of Basic Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran., Khamesipour A; Center for Research and Training in Skin Diseases and Leprosy, Tehran University of Medical Sciences, Tehran, Iran., Tavakkol-Afshari J; Immunogenetics and Tissue Culture Department, Immunology Research Center, Bu-Ali Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran., Akhoundi B; Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, P.O. Box 14155-6446, Tehran, Iran., Abbaszadeh-Afshar MJ; Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, P.O. Box 14155-6446, Tehran, Iran., Alizadeh Z; Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, P.O. Box 14155-6446, Tehran, Iran., Eskandari SE; Center for Research and Training in Skin Diseases and Leprosy, Tehran University of Medical Sciences, Tehran, Iran., Asl AD; Department of Parasitology and Entomology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran., Razmi GR; Department of Pathobiology, School Veterinary Medicine, Ferdowsi University of Mashhad, P.O. Box 91775-1793, Mashhad, Iran. Electronic address: razmi@um.ac.ir.
المصدر: Comparative immunology, microbiology and infectious diseases [Comp Immunol Microbiol Infect Dis] 2023 Jun; Vol. 97, pp. 101984. Date of Electronic Publication: 2023 Apr 25.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Science Ltd Country of Publication: England NLM ID: 7808924 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1878-1667 (Electronic) Linking ISSN: 01479571 NLM ISO Abbreviation: Comp Immunol Microbiol Infect Dis Subsets: MEDLINE
أسماء مطبوعة: Publication: Exeter : Elsevier Science Ltd
Original Publication: Oxford, Elmsford, N. Y., Pergamon Press.
مواضيع طبية MeSH: Leishmaniasis, Visceral*/prevention & control , Leishmaniasis, Visceral*/veterinary , Leishmaniasis, Visceral*/diagnosis , Leishmania infantum* , Leishmaniasis*/veterinary , Leishmaniasis Vaccines* , Dog Diseases*/parasitology, Animals ; Dogs ; Interleukin-17 ; Trimethoprim, Sulfamethoxazole Drug Combination ; CD8-Positive T-Lymphocytes ; Cytokines/metabolism ; Vaccines, Attenuated
مستخلص: This study was designed to evaluate the safety, immunogenicity, and efficacy of a single dose of L. infantum (LiCen -/- ) live attenuated candidate vaccine against canine leishmaniasis (CanL). Eighteen healthy domestic dogs with no anti-Leishmania antibodies and negative leishmanin skin test (LST) were randomly inoculated intravenously with either L. infantum (LiCen -/- ) vaccine candidate in 10 dogs or phosphate-buffered saline (PBS) in 8 dogs. The safety, immunogenicity, and efficacy rate of L. infantum (LiCen -/- ) vaccine candidate against CanL were evaluated by different criteria, including clinical manifestations, injection-site lesion, hematology and biochemistry values, anti-Leishmania antibodies using direct agglutination test (DAT), delayed-type hypersensitivity (DTH) using LST, and CD4 + and CD8 + T-cells subsets, as well as by measuring interferon (IFN-γ), interleukin (IL-23), IL-17, and IL-10 cytokines. Spleen aspiration and detection of Leishmania parasite using parasitological examinations (microscopy and culture) were performed in both vaccinated and control groups. Two months after intervention, each dog was challenged intraperitoneally (IP) with wide type (WT) L. infantum. Two-month follow-up post vaccination showed no clinical signs and serious side effects associated with the vaccination. A significant increase was found in the expression of IL-17, CD4 + , and CD8 + gene transcripts in PBMCs, as well as increased levels of Th1 cytokines, and reduction of Th2 cytokine. The efficacy of the vaccine candidate was calculated to be 42.85%. While the time window for assessing the vaccine's effectiveness was too limited to draw any real conclusions but the preliminary results showed a moderate efficacy rate due to inoculation a single dose of L. infantum (LiCen -/- ) vaccine candidate. Further investigations with more sample sizes and multiple doses of the vaccine candidate using natural challenges in the endemic areas of CanL are recommended.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or persona,relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier Ltd. All rights reserved.)
فهرسة مساهمة: Keywords: Domestic dog; Efficacy; Immunogenicity; Leishmania infantum centrin gene knockout; Safety
المشرفين على المادة: 0 (Interleukin-17)
8064-90-2 (Trimethoprim, Sulfamethoxazole Drug Combination)
0 (Cytokines)
0 (Leishmaniasis Vaccines)
0 (Vaccines, Attenuated)
تواريخ الأحداث: Date Created: 20230429 Date Completed: 20230529 Latest Revision: 20230529
رمز التحديث: 20230529
DOI: 10.1016/j.cimid.2023.101984
PMID: 37119594
قاعدة البيانات: MEDLINE
الوصف
تدمد:1878-1667
DOI:10.1016/j.cimid.2023.101984