دورية أكاديمية

Microwave resonator-based sensor system for specific antibody detection.

التفاصيل البيبلوغرافية
العنوان: Microwave resonator-based sensor system for specific antibody detection.
المؤلفون: Guliy OI; Institute of Biochemistry and Physiology of Plants and Microorganisms - Subdivision of the Federal State Budgetary Research Institution Saratov Federal Scientific Centre of the Russian Academy of Sciences (IBPPM RAS), Saratov 410049, Russia. Electronic address: guliy_olga@mail.ru., Zaitsev BD; Kotelnikov Institute of Radio Engineering and Electronics, Russian Academy of Sciences, Saratov Branch, Saratov 410019, Russia., Smirnov AV; Kotelnikov Institute of Radio Engineering and Electronics, Russian Academy of Sciences, Moscow 125009, Russia., Karavaeva OA; Institute of Biochemistry and Physiology of Plants and Microorganisms - Subdivision of the Federal State Budgetary Research Institution Saratov Federal Scientific Centre of the Russian Academy of Sciences (IBPPM RAS), Saratov 410049, Russia., Burygin GL; Institute of Biochemistry and Physiology of Plants and Microorganisms - Subdivision of the Federal State Budgetary Research Institution Saratov Federal Scientific Centre of the Russian Academy of Sciences (IBPPM RAS), Saratov 410049, Russia., Borodina IA; Kotelnikov Institute of Radio Engineering and Electronics, Russian Academy of Sciences, Saratov Branch, Saratov 410019, Russia.
المصدر: International journal of biological macromolecules [Int J Biol Macromol] 2023 Jul 01; Vol. 242 (Pt 1), pp. 124613. Date of Electronic Publication: 2023 Apr 27.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: Netherlands NLM ID: 7909578 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0003 (Electronic) Linking ISSN: 01418130 NLM ISO Abbreviation: Int J Biol Macromol Subsets: MEDLINE
أسماء مطبوعة: Publication: Amsterdam : Elsevier
Original Publication: Guildford, Eng., IPC Science and Technology Press.
مواضيع طبية MeSH: Antibodies, Bacterial*/analysis , Antigen-Antibody Complex*/analysis , Biosensing Techniques* , Microwaves* , Antibody Specificity*, Antigen-Antibody Reactions ; Azospirillum brasilense ; Azospirillum lipoferum
مستخلص: An antibody-detecting sensor is described that is based on a microwave electrodynamic resonator. A polystyrene film with immobilized bacteria deposited on a lithium niobate plate was placed at one end of the resonator and was used as the sensing element. The second end was electrically shorted. The frequency and depth of the reflection coefficient S 11 for three resonances in the range 6.5-8.5 GHz were used as an analytical signal to examine antibody interactions with bacteria and determine the time required for cell immobilization. The sensor distinguished between situations in which bacteria interacted with specific antibodies and those in which no such interaction occurred (control). Although the cell-antibody interaction changed the frequency and depth of the second and third resonance peaks, the parameters of the first resonance peak did not change. The interaction of cells with nonspecific antibodies did not change the parameters of any of the peaks. These results are promising for use in the design of methods to detect specific antibodies, which can supplement the existing methods of antibody analysis.
Competing Interests: Declaration of competing interest There is no conflict of interest about this article. Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier B.V. All rights reserved.)
فهرسة مساهمة: Keywords: Antibodies; Antibody detection; Antigen–antibody complex; Bacteria; Microwave resonator; Permittivity; Polystyrene films; Reflection coefficient; Resonance peaks
المشرفين على المادة: 0 (Antibodies, Bacterial)
0 (Antigen-Antibody Complex)
تواريخ الأحداث: Date Created: 20230429 Date Completed: 20231103 Latest Revision: 20231103
رمز التحديث: 20231103
DOI: 10.1016/j.ijbiomac.2023.124613
PMID: 37119881
قاعدة البيانات: MEDLINE
الوصف
تدمد:1879-0003
DOI:10.1016/j.ijbiomac.2023.124613