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Dynamic epistasis analysis reveals how chromatin remodeling regulates transcriptional bursting.

التفاصيل البيبلوغرافية
العنوان: Dynamic epistasis analysis reveals how chromatin remodeling regulates transcriptional bursting.
المؤلفون: Brouwer I; Division of Gene Regulation, the Netherlands Cancer Institute, Oncode Institute, Amsterdam, the Netherlands., Kerklingh E; Division of Gene Regulation, the Netherlands Cancer Institute, Oncode Institute, Amsterdam, the Netherlands., van Leeuwen F; Division of Gene Regulation, the Netherlands Cancer Institute, Amsterdam, the Netherlands.; Department of Medical Biology, Amsterdam UMC, University of Amsterdam, Amsterdam, the Netherlands., Lenstra TL; Division of Gene Regulation, the Netherlands Cancer Institute, Oncode Institute, Amsterdam, the Netherlands. t.lenstra@nki.nl.
المصدر: Nature structural & molecular biology [Nat Struct Mol Biol] 2023 May; Vol. 30 (5), pp. 692-702. Date of Electronic Publication: 2023 May 01.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Nature Pub. Group Country of Publication: United States NLM ID: 101186374 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1545-9985 (Electronic) Linking ISSN: 15459985 NLM ISO Abbreviation: Nat Struct Mol Biol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: New York : Nature Pub. Group, c2004-
مواضيع طبية MeSH: Nucleosomes*/metabolism , Saccharomyces cerevisiae Proteins*/genetics , Saccharomyces cerevisiae Proteins*/metabolism, Chromatin Assembly and Disassembly ; Epistasis, Genetic ; Transcription Factors/metabolism ; Chromatin/metabolism ; Saccharomyces cerevisiae/genetics ; Saccharomyces cerevisiae/metabolism ; Transcription, Genetic
مستخلص: Transcriptional bursting has been linked to the stochastic positioning of nucleosomes. However, how bursting is regulated by the remodeling of promoter nucleosomes is unknown. Here, we use single-molecule live-cell imaging of GAL10 transcription in Saccharomyces cerevisiae to measure how bursting changes upon combined perturbations of chromatin remodelers, the transcription factor Gal4 and preinitiation complex components. Using dynamic epistasis analysis, we reveal how the remodeling of different nucleosomes regulates transcriptional bursting parameters. At the nucleosome covering the Gal4 binding sites, RSC and Gal4 binding synergistically facilitate each burst. Conversely, nucleosome remodeling at the TATA box controls only the first burst upon galactose induction. At canonical TATA boxes, the nucleosomes are displaced by TBP binding to allow for transcription activation even in the absence of remodelers. Overall, our results reveal how promoter nucleosome remodeling together with Gal4 and preinitiation complex binding regulates transcriptional bursting.
(© 2023. The Author(s).)
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المشرفين على المادة: 0 (Nucleosomes)
0 (Transcription Factors)
0 (Chromatin)
0 (Saccharomyces cerevisiae Proteins)
تواريخ الأحداث: Date Created: 20230501 Date Completed: 20230519 Latest Revision: 20230630
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC10191856
DOI: 10.1038/s41594-023-00981-1
PMID: 37127821
قاعدة البيانات: MEDLINE
الوصف
تدمد:1545-9985
DOI:10.1038/s41594-023-00981-1