دورية أكاديمية
Irisin inhibits tenocyte response to inflammation in vitro: New insights into tendon-muscle cross-talk.
| العنوان: | Irisin inhibits tenocyte response to inflammation in vitro: New insights into tendon-muscle cross-talk. |
|---|---|
| المؤلفون: | Di Giacomo G; Laboratory for Regenerative Orthopaedics, Research Unit of Orthopaedic and Trauma Surgery, Department of Medicine and Surgery, Università Campus Bio-Medico di Roma, Rome, Italy., Vadalà G; Laboratory for Regenerative Orthopaedics, Research Unit of Orthopaedic and Trauma Surgery, Department of Medicine and Surgery, Università Campus Bio-Medico di Roma, Rome, Italy.; Operative Research Unit of Orthopaedic and Trauma Surgery, Fondazione Policlinico Universitario Campus Bio-Medico, Rome, Italy., Ambrosio L; Laboratory for Regenerative Orthopaedics, Research Unit of Orthopaedic and Trauma Surgery, Department of Medicine and Surgery, Università Campus Bio-Medico di Roma, Rome, Italy.; Operative Research Unit of Orthopaedic and Trauma Surgery, Fondazione Policlinico Universitario Campus Bio-Medico, Rome, Italy., Cicione C; Laboratory for Regenerative Orthopaedics, Research Unit of Orthopaedic and Trauma Surgery, Department of Medicine and Surgery, Università Campus Bio-Medico di Roma, Rome, Italy., Tilotta V; Laboratory for Regenerative Orthopaedics, Research Unit of Orthopaedic and Trauma Surgery, Department of Medicine and Surgery, Università Campus Bio-Medico di Roma, Rome, Italy., Cannata F; Research Unit of Endocrinology and Diabetes, Università Campus Bio-Medico di Roma, Rome, Italy., Russo F; Laboratory for Regenerative Orthopaedics, Research Unit of Orthopaedic and Trauma Surgery, Department of Medicine and Surgery, Università Campus Bio-Medico di Roma, Rome, Italy.; Operative Research Unit of Orthopaedic and Trauma Surgery, Fondazione Policlinico Universitario Campus Bio-Medico, Rome, Italy., Papalia R; Laboratory for Regenerative Orthopaedics, Research Unit of Orthopaedic and Trauma Surgery, Department of Medicine and Surgery, Università Campus Bio-Medico di Roma, Rome, Italy.; Operative Research Unit of Orthopaedic and Trauma Surgery, Fondazione Policlinico Universitario Campus Bio-Medico, Rome, Italy., Denaro V; Operative Research Unit of Orthopaedic and Trauma Surgery, Fondazione Policlinico Universitario Campus Bio-Medico, Rome, Italy. |
| المصدر: | Journal of orthopaedic research : official publication of the Orthopaedic Research Society [J Orthop Res] 2023 Oct; Vol. 41 (10), pp. 2195-2204. Date of Electronic Publication: 2023 May 13. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Wiley Country of Publication: United States NLM ID: 8404726 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1554-527X (Electronic) Linking ISSN: 07360266 NLM ISO Abbreviation: J Orthop Res Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2006- : Hoboken, NJ : Wiley Original Publication: New York, N.Y. : Raven Press, [c1983- |
| مواضيع طبية MeSH: | Fibronectins*/metabolism , Tendinopathy*/metabolism, Humans ; Tumor Necrosis Factor-alpha/metabolism ; Tenocytes/metabolism ; Quality of Life ; Tendons/pathology ; Inflammation/metabolism ; Muscles/pathology |
| مستخلص: | Tendinopathy is one of the most common musculoskeletal disorders with significant repercussions on quality of life and sport activities. Physical exercise (PE) is considered the first-line approach to treat tendinopathy due renowned mechanobiological effects on tenocytes. Irisin, a recently identified myokine released during PE, has been recognized for several beneficial effects towards muscle, cartilage, bone, and intervertebral disc tissues. The aim of this study was to evaluate the effects of irisin on human primary tenocytes (hTCs) in vitro. Human tendons were harvested from specimens of patients undergoing anterior cruciate ligament reconstruction (n = 4). After isolation and expansion, hTCs were treated with RPMI medium (negative control), interleukin (IL)-1β or tumor necrosis factor-α (TNF-α) (positive controls; 10 ng/mL), irisin (5, 10, 25 ng/mL), IL-1β or TNF-α pretreatment and subsequent co-treatment with irisin, pretreatment with irisin and subsequent co-treatment with IL-1β or TNF-α. hTC metabolic activity, proliferation, and nitrite production were evaluated. Detection of unphosphorylated and phosphorylated p38 and ERK was performed. Tissue samples were analyzed by histology and immunohistochemistry to evaluate irisin αVβ5 receptor expression. Irisin significantly increased hTC proliferation and metabolic activity, while reducing the production of nitrites both before and after the addition of IL-1β and TNF-α. Interestingly, irisin reduced p-p38 and pERK levels in inflamed hTCs. The αVβ5 receptor was uniformly expressed on hTC plasma membranes, supporting the potential binding of irisin. This is the first study reporting the capacity of irisin to target hTCs and modulating their response to inflammatory stresses, possibly orchestrating a biological crosstalk between the muscle and tendon. (© 2023 The Authors. Journal of Orthopaedic Research® published by Wiley Periodicals LLC on behalf of Orthopaedic Research Society.) |
| References: | Millar NL, Silbernagel KG, Thorborg K, et al. Tendinopathy. Nat Rev Dis Primers. 2021;7:1. Cannata F, Vadalà G, Ambrosio L, et al. The impact of type 2 diabetes on the development of tendinopathy. Diabetes Metab Res Rev. 2020;37:6. Millar NL, Hueber AJ, Reilly JH, et al. Inflammation is present in early human tendinopathy. Am J Sports Med. 2010;38:2085-2091. Bohm S, Mersmann F, Arampatzis A. Human tendon adaptation in response to mechanical loading: a systematic review and meta-analysis of exercise intervention studies on healthy adults. Sports Med Open. 2015;1:7. Magnusson SP, Langberg H, Kjaer M. The pathogenesis of tendinopathy: balancing the response to loading. Nat Rev Rheumatol. 2010;6:262-268. Gomarasca M, Banfi G, Lombardi G. Myokines: the endocrine coupling of skeletal muscle and bone. Adv Clin Chem. 2020;94:155-218. Boström P, Wu J, Jedrychowski MP, et al. A PGC1-α-dependent myokine that drives brown-fat-like development of white fat and thermogenesis. Nature. 2012;481:463-468. Colaianni G, Cinti S, Colucci S, Grano M. Irisin and musculoskeletal health. Ann NY Acad Sci. 2017;1402:5-9. Chen N, Li Q, Liu J, Jia S. Irisin, an exercise-induced myokine as a metabolic regulator: an updated narrative review. Diabetes Metab Res Rev. 2016;32:51-59. Colaianni G, Sanesi L, Storlino G, Brunetti G, Colucci S, Grano M. Irisin and bone: from preclinical studies to the evaluation of its circulating levels in different populations of human subjects. Cells. 2019;8:451. Vadalà G, Di Giacomo G, Ambrosio L, et al. Irisin recovers osteoarthritic chondrocytes in vitro. Cells. 2020;9:1478. Vadalà G, Di Giacomo G, Ambrosio L, et al. 2022. The Effect of Irisin on Human Nucleus Pulposus Cells: New Insights into the Biological Crosstalk between the Muscle and Intervertebral Disc. Spine Publish Ahead of Print. Waseem R, Shamsi A, Mohammad T, et al. FNDC5/Irisin: physiology and pathophysiology. Molecules. 2022;27:1118. Tilotta V, Vadalà G, Ambrosio L, et al. Mesenchymal stem cell-derived secretome enhances nucleus pulposus cell metabolism and modulates extracellular matrix gene expression in vitro. Front Bioeng Biotechnol. 2023;11:1152207. Papalia R, Cicione C, Russo F, et al. Does vancomycin wrapping in anterior cruciate ligament reconstruction affect tenocyte activity in vitro? Antibiotics. 2021;10:1087. Cicione C, Vadalà G, Di Giacomo G, et al. Micro-fragmented and nanofat adipose tissue derivatives: in vitro qualitative and quantitative analysis. Front Bioeng Biotechnol. 2023;11:911600. Koskinen SOA, Heinemeier KM, Olesen JL, Langberg H, Kjaer M. Physical exercise can influence local levels of matrix metalloproteinases and their inhibitors in tendon-related connective tissue. J Appl Physiol. 2004;96:861-864. Subramanian A, Schilling TF. Thrombospondin-4 controls matrix assembly during development and repair of myotendinous junctions. eLife. 2014;3:e02372. Zhang J, Pan T, Liu Y, Wang JHC. Mouse treadmill running enhances tendons by expanding the pool of tendon stem cells (TSCs) and TSC-related cellular production of collagen. J Orthop Res. 2010;28:1178-1183. Xu L, Chen Z, Geng T, et al. Irisin promotes the proliferation and tenogenic differentiation of rat tendon-derived stem/progenitor cells via activating YAP/TAZ. In Vitro Cell Dev Biol Anim. 2022;8(8):658-668. Vasta S, Di Martino A, Zampogna B, Torre G, Papalia R, Denaro V. Role of VEGF, nitric oxide, and sympathetic neurotransmitters in the pathogenesis of tendinopathy: a review of the current evidences. Front Aging Neurosci. 2016;8:186. Millar NL, Murrell GAC, McInnes IB. Inflammatory mechanisms in tendinopathy-towards translation. Nat Rev Rheumatol. 2017;13:110-122. John T, Lodka D, Kohl B, et al. 2010. Effect of pro-inflammatory and immunoregulatory cytokines on human tenocytes. J Orthop Surg Res. Briolay A, El Jamal A, Arnolfo P, et al. Enhanced BMP-2/BMP-4 ratio in patients with peripheral spondyloarthritis and in cytokine- and stretch-stimulated mouse chondrocytes. Arthritis Res Ther. 2020;22:234. Jiang K, Ye W, Bai Q, Cai J, Wu H, Li X. Therapeutic role of miR-30a in lipoteichoic acid-induced endometritis via targeting the MyD88/Nox2/ROS signaling. Oxid Med Cell Longevity. 2021;2021:1-11. Rabiee F, Lachinani L, Ghaedi S, Nasr-Esfahani MH, Megraw TL, Ghaedi K. New insights into the cellular activities of Fndc5/Irisin and its signaling pathways. Cell Biosci. 2020;10:51. Wang F-S, Kuo C-W, Ko J-Y, et al. Irisin mitigates oxidative stress, chondrocyte dysfunction and osteoarthritis development through regulating mitochondrial integrity and autophagy. Antioxidants. 2020;9:810. Kim H, Wrann CD, Jedrychowski M, et al. Irisin mediates effects on bone and fat via αV integrin receptors. Cell. 2018;175:1756-1768. Zhang J, Ren Y, Bi J, et al. Involvement of kindlin-2 in irisin's protection against ischaemia reperfusion-induced liver injury in high-fat diet-fed mice. J Cell Mol Med. 2020;24:13081-13092. Scott A, Cook JL, Hart DA, Walker DC, Duronio V, Khan KM. Tenocyte responses to mechanical loading in vivo: a role for local insulin-like growth factor 1 signaling in early tendinosis in rats. Arthritis Rheum. 2007;56:871-881. Millar NL, Reilly JH, Kerr SC, et al. Hypoxia: a critical regulator of early human tendinopathy. Ann Rheum Dis. 2012;71:302-310. |
| فهرسة مساهمة: | Keywords: irisin; muscle; physical exercise; tendinopathy; tendon; tenocyte |
| المشرفين على المادة: | 0 (Fibronectins) 0 (Tumor Necrosis Factor-alpha) |
| تواريخ الأحداث: | Date Created: 20230503 Date Completed: 20230920 Latest Revision: 20230925 |
| رمز التحديث: | 20230925 |
| DOI: | 10.1002/jor.25586 |
| PMID: | 37132159 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1554-527X |
|---|---|
| DOI: | 10.1002/jor.25586 |