دورية أكاديمية
Irisin inhibits tenocyte response to inflammation in vitro: New insights into tendon-muscle cross-talk.
العنوان: | Irisin inhibits tenocyte response to inflammation in vitro: New insights into tendon-muscle cross-talk. |
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المؤلفون: | Di Giacomo G; Laboratory for Regenerative Orthopaedics, Research Unit of Orthopaedic and Trauma Surgery, Department of Medicine and Surgery, Università Campus Bio-Medico di Roma, Rome, Italy., Vadalà G; Laboratory for Regenerative Orthopaedics, Research Unit of Orthopaedic and Trauma Surgery, Department of Medicine and Surgery, Università Campus Bio-Medico di Roma, Rome, Italy.; Operative Research Unit of Orthopaedic and Trauma Surgery, Fondazione Policlinico Universitario Campus Bio-Medico, Rome, Italy., Ambrosio L; Laboratory for Regenerative Orthopaedics, Research Unit of Orthopaedic and Trauma Surgery, Department of Medicine and Surgery, Università Campus Bio-Medico di Roma, Rome, Italy.; Operative Research Unit of Orthopaedic and Trauma Surgery, Fondazione Policlinico Universitario Campus Bio-Medico, Rome, Italy., Cicione C; Laboratory for Regenerative Orthopaedics, Research Unit of Orthopaedic and Trauma Surgery, Department of Medicine and Surgery, Università Campus Bio-Medico di Roma, Rome, Italy., Tilotta V; Laboratory for Regenerative Orthopaedics, Research Unit of Orthopaedic and Trauma Surgery, Department of Medicine and Surgery, Università Campus Bio-Medico di Roma, Rome, Italy., Cannata F; Research Unit of Endocrinology and Diabetes, Università Campus Bio-Medico di Roma, Rome, Italy., Russo F; Laboratory for Regenerative Orthopaedics, Research Unit of Orthopaedic and Trauma Surgery, Department of Medicine and Surgery, Università Campus Bio-Medico di Roma, Rome, Italy.; Operative Research Unit of Orthopaedic and Trauma Surgery, Fondazione Policlinico Universitario Campus Bio-Medico, Rome, Italy., Papalia R; Laboratory for Regenerative Orthopaedics, Research Unit of Orthopaedic and Trauma Surgery, Department of Medicine and Surgery, Università Campus Bio-Medico di Roma, Rome, Italy.; Operative Research Unit of Orthopaedic and Trauma Surgery, Fondazione Policlinico Universitario Campus Bio-Medico, Rome, Italy., Denaro V; Operative Research Unit of Orthopaedic and Trauma Surgery, Fondazione Policlinico Universitario Campus Bio-Medico, Rome, Italy. |
المصدر: | Journal of orthopaedic research : official publication of the Orthopaedic Research Society [J Orthop Res] 2023 Oct; Vol. 41 (10), pp. 2195-2204. Date of Electronic Publication: 2023 May 13. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Wiley Country of Publication: United States NLM ID: 8404726 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1554-527X (Electronic) Linking ISSN: 07360266 NLM ISO Abbreviation: J Orthop Res Subsets: MEDLINE |
أسماء مطبوعة: | Publication: 2006- : Hoboken, NJ : Wiley Original Publication: New York, N.Y. : Raven Press, [c1983- |
مواضيع طبية MeSH: | Fibronectins*/metabolism , Tendinopathy*/metabolism, Humans ; Tumor Necrosis Factor-alpha/metabolism ; Tenocytes/metabolism ; Quality of Life ; Tendons/pathology ; Inflammation/metabolism ; Muscles/pathology |
مستخلص: | Tendinopathy is one of the most common musculoskeletal disorders with significant repercussions on quality of life and sport activities. Physical exercise (PE) is considered the first-line approach to treat tendinopathy due renowned mechanobiological effects on tenocytes. Irisin, a recently identified myokine released during PE, has been recognized for several beneficial effects towards muscle, cartilage, bone, and intervertebral disc tissues. The aim of this study was to evaluate the effects of irisin on human primary tenocytes (hTCs) in vitro. Human tendons were harvested from specimens of patients undergoing anterior cruciate ligament reconstruction (n = 4). After isolation and expansion, hTCs were treated with RPMI medium (negative control), interleukin (IL)-1β or tumor necrosis factor-α (TNF-α) (positive controls; 10 ng/mL), irisin (5, 10, 25 ng/mL), IL-1β or TNF-α pretreatment and subsequent co-treatment with irisin, pretreatment with irisin and subsequent co-treatment with IL-1β or TNF-α. hTC metabolic activity, proliferation, and nitrite production were evaluated. Detection of unphosphorylated and phosphorylated p38 and ERK was performed. Tissue samples were analyzed by histology and immunohistochemistry to evaluate irisin αVβ5 receptor expression. Irisin significantly increased hTC proliferation and metabolic activity, while reducing the production of nitrites both before and after the addition of IL-1β and TNF-α. Interestingly, irisin reduced p-p38 and pERK levels in inflamed hTCs. The αVβ5 receptor was uniformly expressed on hTC plasma membranes, supporting the potential binding of irisin. This is the first study reporting the capacity of irisin to target hTCs and modulating their response to inflammatory stresses, possibly orchestrating a biological crosstalk between the muscle and tendon. (© 2023 The Authors. Journal of Orthopaedic Research® published by Wiley Periodicals LLC on behalf of Orthopaedic Research Society.) |
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فهرسة مساهمة: | Keywords: irisin; muscle; physical exercise; tendinopathy; tendon; tenocyte |
المشرفين على المادة: | 0 (Fibronectins) 0 (Tumor Necrosis Factor-alpha) |
تواريخ الأحداث: | Date Created: 20230503 Date Completed: 20230920 Latest Revision: 20230925 |
رمز التحديث: | 20230925 |
DOI: | 10.1002/jor.25586 |
PMID: | 37132159 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1554-527X |
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DOI: | 10.1002/jor.25586 |