دورية أكاديمية
أعرض في EDS

Long non-coding RNAs are essential for Schistosoma mansoni pairing-dependent adult worm homeostasis and fertility.

التفاصيل البيبلوغرافية
العنوان: Long non-coding RNAs are essential for Schistosoma mansoni pairing-dependent adult worm homeostasis and fertility.
المؤلفون: Silveira GO; Laboratório de Ciclo Celular, Instituto Butantan, São Paulo, São Paulo, Brazil.; Instituto de Química, Universidade de São Paulo, São Paulo, São Paulo, Brazil., Coelho HS; Laboratório de Ciclo Celular, Instituto Butantan, São Paulo, São Paulo, Brazil., Pereira ASA; Laboratório de Ciclo Celular, Instituto Butantan, São Paulo, São Paulo, Brazil.; Instituto de Química, Universidade de São Paulo, São Paulo, São Paulo, Brazil., Miyasato PA; Laboratório de Parasitologia, Instituto Butantan, São Paulo, São Paulo, Brazil., Santos DW; Laboratório de Ciclo Celular, Instituto Butantan, São Paulo, São Paulo, Brazil.; Instituto de Química, Universidade de São Paulo, São Paulo, São Paulo, Brazil., Maciel LF; Laboratório de Ciclo Celular, Instituto Butantan, São Paulo, São Paulo, Brazil., Olberg GGG; Laboratório de Ciclo Celular, Instituto Butantan, São Paulo, São Paulo, Brazil., Tahira AC; Laboratório de Ciclo Celular, Instituto Butantan, São Paulo, São Paulo, Brazil., Nakano E; Laboratório de Parasitologia, Instituto Butantan, São Paulo, São Paulo, Brazil., Oliveira MLS; Laboratório de Bacteriologia, Instituto Butantan, São Paulo, São Paulo, Brazil., Amaral MS; Laboratório de Ciclo Celular, Instituto Butantan, São Paulo, São Paulo, Brazil., Verjovski-Almeida S; Laboratório de Ciclo Celular, Instituto Butantan, São Paulo, São Paulo, Brazil.; Instituto de Química, Universidade de São Paulo, São Paulo, São Paulo, Brazil.
المصدر: PLoS pathogens [PLoS Pathog] 2023 May 05; Vol. 19 (5), pp. e1011369. Date of Electronic Publication: 2023 May 05 (Print Publication: 2023).
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Public Library of Science Country of Publication: United States NLM ID: 101238921 Publication Model: eCollection Cited Medium: Internet ISSN: 1553-7374 (Electronic) Linking ISSN: 15537366 NLM ISO Abbreviation: PLoS Pathog Subsets: MEDLINE
أسماء مطبوعة: Original Publication: San Francisco, CA : Public Library of Science, c2005-
مواضيع طبية MeSH: RNA, Long Noncoding*/genetics , Parasites*/genetics , Schistosomiasis mansoni*/parasitology, Male ; Female ; Animals ; Mice ; Schistosoma mansoni/genetics ; Fertility/genetics ; Reproduction ; Mammals
مستخلص: The trematode parasite Schistosoma mansoni causes schistosomiasis, which affects over 200 million people worldwide. Schistosomes are dioecious, with egg laying depending on the females' obligatory pairing with males. Long non-coding RNAs (lncRNAs) are transcripts longer than 200 nucleotides with low or no protein-coding potential that have been involved in other species with reproduction, stem cell maintenance, and drug resistance. In S. mansoni, we recently showed that the knockdown of one lncRNA affects the pairing status of these parasites. Here, we re-analyzed public RNA-Seq data from paired and unpaired adult male and female worms and their gonads, obtained from mixed-sex or single-sex cercariae infections, and found thousands of differentially expressed pairing-dependent lncRNAs among the 23 biological samples that were compared. The expression levels of selected lncRNAs were validated by RT-qPCR using an in vitro unpairing model. In addition, the in vitro silencing of three selected lncRNAs showed that knockdown of these pairing-dependent lncRNAs reduced cell proliferation in adult worms and their gonads, and are essential for female vitellaria maintenance, reproduction, and/or egg development. Remarkably, in vivo silencing of each of the three selected lncRNAs significantly reduced worm burden in infected mice by 26 to 35%. Whole mount in situ hybridization experiments showed that these pairing-dependent lncRNAs are expressed in reproductive tissues. These results show that lncRNAs are key components intervening in S. mansoni adult worm homeostasis, which affects pairing status and survival in the mammalian host, thus presenting great potential as new therapeutic target candidates.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: © 2023 Silveira et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
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المشرفين على المادة: 0 (RNA, Long Noncoding)
تواريخ الأحداث: Date Created: 20230505 Date Completed: 20230519 Latest Revision: 20230521
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC10191282
DOI: 10.1371/journal.ppat.1011369
PMID: 37146077
قاعدة البيانات: MEDLINE
الوصف
تدمد:1553-7374
DOI:10.1371/journal.ppat.1011369