دورية أكاديمية
Long non-coding RNAs are essential for Schistosoma mansoni pairing-dependent adult worm homeostasis and fertility.
العنوان: | Long non-coding RNAs are essential for Schistosoma mansoni pairing-dependent adult worm homeostasis and fertility. |
---|---|
المؤلفون: | Silveira GO; Laboratório de Ciclo Celular, Instituto Butantan, São Paulo, São Paulo, Brazil.; Instituto de Química, Universidade de São Paulo, São Paulo, São Paulo, Brazil., Coelho HS; Laboratório de Ciclo Celular, Instituto Butantan, São Paulo, São Paulo, Brazil., Pereira ASA; Laboratório de Ciclo Celular, Instituto Butantan, São Paulo, São Paulo, Brazil.; Instituto de Química, Universidade de São Paulo, São Paulo, São Paulo, Brazil., Miyasato PA; Laboratório de Parasitologia, Instituto Butantan, São Paulo, São Paulo, Brazil., Santos DW; Laboratório de Ciclo Celular, Instituto Butantan, São Paulo, São Paulo, Brazil.; Instituto de Química, Universidade de São Paulo, São Paulo, São Paulo, Brazil., Maciel LF; Laboratório de Ciclo Celular, Instituto Butantan, São Paulo, São Paulo, Brazil., Olberg GGG; Laboratório de Ciclo Celular, Instituto Butantan, São Paulo, São Paulo, Brazil., Tahira AC; Laboratório de Ciclo Celular, Instituto Butantan, São Paulo, São Paulo, Brazil., Nakano E; Laboratório de Parasitologia, Instituto Butantan, São Paulo, São Paulo, Brazil., Oliveira MLS; Laboratório de Bacteriologia, Instituto Butantan, São Paulo, São Paulo, Brazil., Amaral MS; Laboratório de Ciclo Celular, Instituto Butantan, São Paulo, São Paulo, Brazil., Verjovski-Almeida S; Laboratório de Ciclo Celular, Instituto Butantan, São Paulo, São Paulo, Brazil.; Instituto de Química, Universidade de São Paulo, São Paulo, São Paulo, Brazil. |
المصدر: | PLoS pathogens [PLoS Pathog] 2023 May 05; Vol. 19 (5), pp. e1011369. Date of Electronic Publication: 2023 May 05 (Print Publication: 2023). |
نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
اللغة: | English |
بيانات الدورية: | Publisher: Public Library of Science Country of Publication: United States NLM ID: 101238921 Publication Model: eCollection Cited Medium: Internet ISSN: 1553-7374 (Electronic) Linking ISSN: 15537366 NLM ISO Abbreviation: PLoS Pathog Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: San Francisco, CA : Public Library of Science, c2005- |
مواضيع طبية MeSH: | RNA, Long Noncoding*/genetics , Parasites*/genetics , Schistosomiasis mansoni*/parasitology, Male ; Female ; Animals ; Mice ; Schistosoma mansoni/genetics ; Fertility/genetics ; Reproduction ; Mammals |
مستخلص: | The trematode parasite Schistosoma mansoni causes schistosomiasis, which affects over 200 million people worldwide. Schistosomes are dioecious, with egg laying depending on the females' obligatory pairing with males. Long non-coding RNAs (lncRNAs) are transcripts longer than 200 nucleotides with low or no protein-coding potential that have been involved in other species with reproduction, stem cell maintenance, and drug resistance. In S. mansoni, we recently showed that the knockdown of one lncRNA affects the pairing status of these parasites. Here, we re-analyzed public RNA-Seq data from paired and unpaired adult male and female worms and their gonads, obtained from mixed-sex or single-sex cercariae infections, and found thousands of differentially expressed pairing-dependent lncRNAs among the 23 biological samples that were compared. The expression levels of selected lncRNAs were validated by RT-qPCR using an in vitro unpairing model. In addition, the in vitro silencing of three selected lncRNAs showed that knockdown of these pairing-dependent lncRNAs reduced cell proliferation in adult worms and their gonads, and are essential for female vitellaria maintenance, reproduction, and/or egg development. Remarkably, in vivo silencing of each of the three selected lncRNAs significantly reduced worm burden in infected mice by 26 to 35%. Whole mount in situ hybridization experiments showed that these pairing-dependent lncRNAs are expressed in reproductive tissues. These results show that lncRNAs are key components intervening in S. mansoni adult worm homeostasis, which affects pairing status and survival in the mammalian host, thus presenting great potential as new therapeutic target candidates. Competing Interests: The authors have declared that no competing interests exist. (Copyright: © 2023 Silveira et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.) |
References: | Expert Rev Vaccines. 2014 Apr;13(4):473-87. (PMID: 24606541) Bioinformatics. 2018 Sep 1;34(17):i884-i890. (PMID: 30423086) PLoS Biol. 2019 Feb 22;17(2):e3000154. (PMID: 30794532) Lancet. 2014 Jun 28;383(9936):2253-64. (PMID: 24698483) Lancet Infect Dis. 2017 Feb;17(2):e42-e48. (PMID: 27988094) Mem Inst Oswaldo Cruz. 2002 Oct;97(7):917-40. (PMID: 12471417) PLoS Negl Trop Dis. 2011 Mar 08;5(3):e1009. (PMID: 21408085) Mol Biochem Parasitol. 2017 Jul;215:2-10. (PMID: 27899279) Parasitology. 2001 Mar;122(Pt 3):329-38. (PMID: 11289069) Parasitology. 1997 Jul;115 ( Pt 1):29-32. (PMID: 9226954) Trends Genet. 2017 Oct;33(10):665-676. (PMID: 28870653) Biochem Biophys Res Commun. 2018 Sep 5;503(2):1004-1010. (PMID: 29935182) Cancer Res. 2004 Aug 1;64(15):5245-50. (PMID: 15289330) Clin Chem. 2009 Apr;55(4):611-22. (PMID: 19246619) Genome Biol. 2002 Jun 18;3(7):RESEARCH0034. (PMID: 12184808) Front Genet. 2019 Sep 06;10:796. (PMID: 31552097) Sci Rep. 2020 Dec 9;10(1):21565. (PMID: 33299037) PLoS Negl Trop Dis. 2012;6(2):e1509. (PMID: 22363825) Dev Biol. 2008 May 15;317(2):671-85. (PMID: 18358469) Pharmaceuticals (Basel). 2018 Feb 05;11(1):. (PMID: 29401734) Mol Biochem Parasitol. 2007 Jun;153(2):194-202. (PMID: 17420062) Nature. 2013 Feb 28;494(7438):476-9. (PMID: 23426263) PLoS Negl Trop Dis. 2018 Oct 26;12(10):e0006873. (PMID: 30365505) Parasit Vectors. 2015 Dec 08;8:624. (PMID: 26644133) Genome Biol. 2014;15(12):550. (PMID: 25516281) Am J Trop Med Hyg. 1994 Mar;50(3):281-95. (PMID: 8147487) Parasitology. 1965 Nov;55(4):695-700. (PMID: 4957633) Exp Parasitol. 2008 Apr;118(4):619-23. (PMID: 18237732) PLoS Negl Trop Dis. 2017 Sep 15;11(9):e0005891. (PMID: 28915270) Science. 2020 Sep 25;369(6511):1644-1649. (PMID: 32973030) PLoS Biol. 2019 May 8;17(5):e3000254. (PMID: 31067225) Nat Methods. 2012 Jun 28;9(7):676-82. (PMID: 22743772) Int J Parasitol. 1984 Apr;14(2):183-90. (PMID: 6735582) Front Genet. 2020 Mar 31;11:277. (PMID: 32296461) J Parasitol. 1983 Jun;69(3):559-66. (PMID: 6631627) PLoS Negl Trop Dis. 2010 Oct 19;4(10):e850. (PMID: 20976050) Trends Parasitol. 2018 Mar;34(3):246-260. (PMID: 29276074) Emerg Top Life Sci. 2017 Dec 22;1(6):641-650. (PMID: 33525844) BMC Bioinformatics. 2011 Aug 04;12:323. (PMID: 21816040) J Exp Zool. 1984 Oct;232(1):141-50. (PMID: 6502090) Trends Biotechnol. 2019 Jul;37(7):761-774. (PMID: 30654913) Proc Natl Acad Sci U S A. 1988 Feb;85(4):1033-7. (PMID: 3277187) Parasitol Res. 2022 Apr;121(4):1091-1115. (PMID: 34859292) Int J Parasitol. 2016 Jun;46(7):405-10. (PMID: 27056273) Science. 1921 Mar 11;53(1367):226-8. (PMID: 17734017) Sci Rep. 2021 Aug 19;11(1):16816. (PMID: 34413342) Bioinformatics. 2013 Jan 1;29(1):15-21. (PMID: 23104886) Sci Rep. 2016 Aug 08;6:31150. (PMID: 27499125) Rev Bras Pesqui Med Biol. 1979 Dec;12(6):411-9. (PMID: 542655) PLoS One. 2019 Nov 25;14(11):e0225425. (PMID: 31765429) Annu Rev Biochem. 2020 Jun 20;89:283-308. (PMID: 32569523) Cell. 2022 Apr 28;185(9):1506-1520.e17. (PMID: 35385687) Parasitology. 2020 Apr;147(5):507-515. (PMID: 31965953) Methods. 2001 Dec;25(4):402-8. (PMID: 11846609) Parasitol Today. 1991 Nov;7(11):303-8. (PMID: 15463396) Infect Dis Poverty. 2017 Mar 28;6(1):74. (PMID: 28351414) Front Genet. 2022 Sep 20;13:924877. (PMID: 36204320) Vet Parasitol. 2005 Apr 20;129(1-2):83-7. (PMID: 15817207) Nature. 2009 Jul 16;460(7253):352-8. (PMID: 19606141) Proc Natl Acad Sci U S A. 2019 Oct 29;116(44):22020-22029. (PMID: 31548386) Nat Rev Mol Cell Biol. 2018 Mar;19(3):143-157. (PMID: 29138516) PLoS Negl Trop Dis. 2009 Aug 18;3(8):e504. (PMID: 19688043) RNA Biol. 2019 Nov;16(11):1586-1591. (PMID: 31390943) Antimicrob Agents Chemother. 2017 Apr 24;61(5):. (PMID: 28264841) J Mol Biol. 1990 Oct 5;215(3):403-10. (PMID: 2231712) Front Genet. 2019 Sep 12;10:823. (PMID: 31572441) |
المشرفين على المادة: | 0 (RNA, Long Noncoding) |
تواريخ الأحداث: | Date Created: 20230505 Date Completed: 20230519 Latest Revision: 20230521 |
رمز التحديث: | 20231215 |
مُعرف محوري في PubMed: | PMC10191282 |
DOI: | 10.1371/journal.ppat.1011369 |
PMID: | 37146077 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1553-7374 |
---|---|
DOI: | 10.1371/journal.ppat.1011369 |