دورية أكاديمية
Analyzing Activities of Lytic Polysaccharide Monooxygenases by Liquid Chromatography and Mass Spectrometry.
| العنوان: | Analyzing Activities of Lytic Polysaccharide Monooxygenases by Liquid Chromatography and Mass Spectrometry. |
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| المؤلفون: | Westereng B; Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, Aas, Norway. bjorge.westereng@nmbu.no., Arntzen MØ; Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, Aas, Norway., Østby H; Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, Aas, Norway., Agger JW; Center for BioProcess Engineering, Department of Chemical and Biochemical Engineering, Technical University of Denmark, Lyngby, Denmark., Vaaje-Kolstad G; Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, Aas, Norway., Eijsink VGH; Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, Aas, Norway. |
| المصدر: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2023; Vol. 2657, pp. 27-51. |
| نوع المنشور: | Review; Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Totowa, NJ : Humana Press Original Publication: Clifton, N.J. : Humana Press, |
| مواضيع طبية MeSH: | Mixed Function Oxygenases*/metabolism , Polysaccharides*/metabolism, Chromatography, Liquid ; Mass Spectrometry ; Oxidation-Reduction ; Cellulose/metabolism ; Glycoside Hydrolases/metabolism |
| مستخلص: | Lytic polysaccharide monooxygenases perform oxidative cleavage of glycosidic bonds in various polysaccharides. The majority of LMPOs studied so far possess activity on either cellulose or chitin and analysis of these activities is therefore the main focus of this review. Notably, however, the number of LPMOs that are active on other polysaccharides is increasing. The products generated by LPMOs from cellulose are either oxidized in the downstream end (at C1) or upstream end (at C4), or at both ends. These modifications only result in small structural changes, which makes both chromatographic separation and product identification by mass spectrometry challenging. The changes in physicochemical properties that are associated with oxidation need to be considered when choosing analytical approaches. C1 oxidation leads to a sugar that is no longer reducing but instead has an acidic functionality, whereas C4 oxidation leads to products that are inherently labile at high and low pH and that exist in a keto-gemdiol equilibrium that is strongly shifted towards the gemdiol in aqueous solutions. Partial degradation of C4-oxidized products leads to the formation of native products, which could explain why some authors claim to have observed glycoside hydrolase activity for LPMOs. Notably, apparent glycoside hydrolase activity may also be due to small amounts of contaminating glycoside hydrolases since these normally have much higher catalytic rates than LPMOs. The low catalytic turnover rates of LPMOs necessitate the use of sensitive product detection methods, which limits the analytical possibilities considerably. Modern liquid chromatography and mass spectrometry have become essential tools for evaluating LPMO activity and this chapter provides an overview of available methods together with a few novel tools. The methods described constitute a suite of techniques for analyzing oxidized carbohydrate products, which can be applied to LPMOs as well as other carbohydrate-active redox enzymes. (© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.) |
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| فهرسة مساهمة: | Keywords: Aldonic acid; Gemdiol; High-performance anion-exchange chromatography; Hydrophilic interaction liquid chromatography; Lytic polysaccharide monooxygenase; Porous graphitized carbon |
| المشرفين على المادة: | EC 1.- (Mixed Function Oxygenases) 0 (Polysaccharides) 9004-34-6 (Cellulose) EC 3.2.1.- (Glycoside Hydrolases) |
| تواريخ الأحداث: | Date Created: 20230506 Date Completed: 20230508 Latest Revision: 20230510 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1007/978-1-0716-3151-5_3 |
| PMID: | 37149521 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1940-6029 |
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| DOI: | 10.1007/978-1-0716-3151-5_3 |