دورية أكاديمية
Multivisceral transplantation of pelvic organs in rats.
| العنوان: | Multivisceral transplantation of pelvic organs in rats. |
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| المؤلفون: | Galvao FHF; Laboratory of Medical Investigation 37, Department of Gastroenterology, Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil., Araki J; Division of Plastic and Reconstructive Surgery, Shizuoka Cancer Center Hospital, Shizuoka, Japan., Fonseca ABS; Laboratory of Medical Investigation 37, Department of Gastroenterology, Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil., Cruz RJ Jr; Department of Surgery, University of Pittsburgh, Pittsburgh, PA, United States., Lanchotte C; Laboratory of Medical Investigation 37, Department of Gastroenterology, Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil., Waisberg DR; Laboratory of Medical Investigation 37, Department of Gastroenterology, Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil., Chaib E; Laboratory of Medical Investigation 37, Department of Gastroenterology, Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil., Nacif LS; Laboratory of Medical Investigation 37, Department of Gastroenterology, Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil., Traldi MCC; Laboratory of Medical Investigation 37, Department of Gastroenterology, Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil., de Mello EB; Laboratory of Medical Investigation 37, Department of Gastroenterology, Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil., Andraus W; Laboratory of Medical Investigation 37, Department of Gastroenterology, Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil., Carneiro-D'Albuquerque L; Laboratory of Medical Investigation 37, Department of Gastroenterology, Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil. |
| المصدر: | Frontiers in surgery [Front Surg] 2023 Apr 20; Vol. 10, pp. 1086651. Date of Electronic Publication: 2023 Apr 20 (Print Publication: 2023). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Frontiers Media S.A Country of Publication: Switzerland NLM ID: 101645127 Publication Model: eCollection Cited Medium: Print ISSN: 2296-875X (Print) Linking ISSN: 2296875X NLM ISO Abbreviation: Front Surg Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Original Publication: Lausanne : Frontiers Media S.A., [2014]- |
| مستخلص: | Background: Multivisceral transplantation of pelvic organs would be a potential treatment for severe pelvic floor dysfunction with fecal and urinary incontinence, extensive perineal trauma, or congenital disorders. Here, we describe the microsurgical technique of multivisceral transplantation of pelvic organs, including the pelvic floor, in rats. Donor Operation: We performed a perineal (including the genitalia, anus, muscles, and ligaments) and abdominal incision. The dissection progressed near the pelvic ring, dividing ligaments, muscles, external iliac vessels, and pudendal nerves, allowing pelvic floor mobilization. The aorta and vena cava were isolated distally, preserving the internal iliac and gonadal vessels. The graft containing the skin, muscles, ligaments, bladder, ureter, rectum, anus and vagina, uterus and ovarian (female), or penile, testis and its ducts (male) was removed en bloc , flushed, and cold-stored. Recipient Operation: The infrarenal aorta and vena cava were isolated and donor/recipient aorta-aorta and cava-cava end-to-side microanastomoses were performed. After pelvic floor and viscera removal, we performed microanastomoses between the donor and the recipient ureter, and the rectum and pudenda nerves. The pelvic floor was repositioned in its original position (orthotopic model) or the abdominal wall (heterotopic model). We sacrificed the animals 2 h after surgery. Results: We performed seven orthotopic and four heterotopic transplantations. One animal from the orthotopic model and one from the heterotopic model died because of technical failure. Six orthotopic and three heterotopic recipients survived up to 2 h after transplantation. Conclusion: The microsurgical technique for pelvic floor transplantation in rats is feasible, achieving an early survival rate of 81.82%. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (© 2023 Galvao, Araki, Fonseca, Cruz, Lanchotte, Waisberg, Chaib, Nacif, Traldi, Mello, Andraus and Carneiro-D'Albuquerque.) |
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| فهرسة مساهمة: | Keywords: anal canal; dysphoria; fecal incontinence; gender reassignment; intestinal transplantation; pelvic floor disorders; tissue transplantation; urinary incontinence |
| تواريخ الأحداث: | Date Created: 20230508 Latest Revision: 20230509 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC10159176 |
| DOI: | 10.3389/fsurg.2023.1086651 |
| PMID: | 37151860 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2296-875X |
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| DOI: | 10.3389/fsurg.2023.1086651 |